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Research Article
Achnanthidium bratanense sp. nov. (Bacillariophyceae, Achnanthidiaceae), a new diatom from the Lake Bratan (Bali, Indonesia)
expand article infoDmitry A. Kapustin, Anton M. Glushchenko, Maxim S. Kulikovskiy
‡ Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia
Open Access

Abstract

A new species, Achnanthidium bratanense, is described from Lake Bratan, located on the island of Bali (Indonesia). The morphology of this species was analyzed with light (LM) and scanning electron microscopy (SEM). A. bratanense is characterized by linear-elliptic to nearly elliptic valves with convex margins and rounded, broadly subcapitate apices. The striae of this species are hardly discernable under LM; they are weakly radiate throughout the valve and composed of one to four large transapically elongated areolae of different length and shape. The most similar taxon to A. bratanense is A. macrocephalum, a species described from Sumatra, another Indonesian island. The differences of A. bratanense from similar taxa are discussed.

Keywords

Indonesia, monoraphid diatoms, morphology, new species

Introduction

The genus Achnanthidium Kützing, 1844 is one of the largest genus among monoraphid diatoms. Although it had been described as a separate genus it was considered as a subgenus of Achnanthes Bory, from 1822 until the 90s (Round et al. 1990; Round and Bukhtiyarova 1996). Currently, Achnanthidium includes, according to different estimates, between 139 (Guiry and Guiry 2017) and nearly 200 species (Kociolek et al. 2021a). Revision of the genus continues up to now. Recently, two genera, namely Gogorevia (Kulikovskiy et al. 2020b) and Gomphothidium (Kociolek et al. 2021b) have been segregated from Achnanthidium.

The Achnanthidium taxa are common in different climatic zones all over the world (e.g., Ponader and Potapova 2007; Wojtal et al. 2011; Novais et al. 2015; Karthick et al. 2017; Marquardt et al. 2017; Krahn et al. 2018; Yu et al. 2019). However, their identification is challenging because of the small size of these diatoms, often requiring examination using electron microscopy, and significant variability of diagnostic features (Ponader and Potapova 2007; Hlúbiková et al. 2011).

The number of publications dealing with freshwater diatoms from Indonesia is still rather low. The most comprehensive treatment was made by Hustedt (1937, 1942). Some of his new taxa have been re-examined (e.g., Hamsher et al. 2014; Kapustin et al. 2017, 2020; Kapustin and Kulikovskiy 2018; Wetzel et al. 2019; Kulikovskiy et al. 2020a). Also, a lot of new diatom species were described from Indonesian freshwaters over the last two decades (Bramburger et al. 2006; Kociolek et al. 2018; Kapustin et al. 2019, 2021; Kulikovskiy et al. 2019; Rybak et al. 2019), including two new Achnanthidium species (Tseplik et al. 2021a,b). The aim of this paper is to describe a new monoraphid species, Achnanthidium bratanense sp. nov., from Lake Bratan located on the island of Bali, Indonesia.

Materials and methods

A benthic sample containing Achnanthidium was collected from a volcanic Lake Bratan on 14 November 2010 (08°16.579'S, 115°09.985'E). For general characteristics of this lake see Green et al. (1978). Environmental variables were measured with a Hanna multiparameter probe meter (HANNA HI98128).

The sample was boiled in concentrated hydrogen peroxide (~37%) to dissolve the organic matter. It was then washed with deionized water four times at 12 h intervals. After decanting and filling with deionized water up to 100 ml, the suspension was spread on to coverslips and left to dry at room temperature. Permanent diatom slides were mounted in Naphrax. Light microscopic (LM) observations were performed with a Zeiss Scope A1 microscope equipped with an oil immersion objective (100×/n.a.1.4, differential interference contrast [DIC]) and Zeiss Axio-Cam ERc 5s camera. Valve ultrastructure was examined with a JSM-6510LV scanning electron microscope (Papanin Institute for Biology of Inland Waters RAS, Borok, Russia), operated at 10 kV and 11 mm distance. For scanning electron microscopy (SEM), parts of the suspensions were fixed on aluminum stubs after air-drying. The stubs were sputter coated with 50 nm of gold.

The original sample preserved with Lugol’s solution, as well as cleaned material preserved with 96% ethanol, are housed at the Laboratory of Molecular Systematics of Aquatic Plants, K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences (Moscow, Russia).

Results

Achnanthidium bratanense Kapustin, Glushchenko & Kulikovskiy, sp. nov.

Figures 1, 2

Description

LM (Fig. 1A-T). Valves linear-elliptic to nearly elliptic with convex margins and rounded, broadly subcapitate apices. Frustules rectangular in girdle view and not bent (Fig. 1T). Length 5.0–8.7 µm, breadth 2.7–3.2 µm (n=32). In raphe valves axial area narrow, linear, slightly widening at center. Central area very small in raphe valves, outlined by shortened striae; central area in rapheless valves rhomboid (Fig. 1J). Raphe straight, filiform. In rapheless valves axial area expanded widening towards rhombic central area (Fig. 1E). Striae hardly discernable in LM, weakly radiate (Fig. 1A, H, K, M). Areolae indistinct in LM.

Figure 1. 

A–T Achnanthidium bratanense sp. nov. (LM). A–S size diminution series showing variation in valve outline A holotype specimen A-D, F-I, K, M, N, R raphe valves E, J, L, O-Q, S rapheless valves T frustule in girdle view. Scale bar: 10 μm.

SEM (Fig. 2). Externally, raphe straight, filiform with drop-shaped proximal and distal raphe endings (Fig. 2A, B). Internally, proximal raphe endings deflected in opposite directions, distal raphe endings terminating in helictoglossae (Fig. 2E, F). Striae weakly radiate throughout the valve, 41–44 in 10 µm, and composed of one to four large transapically elongated areolae of different length and shape (from slit-like to irregularly rectangular). Areolae absent along valve margins; mantle with a single row of slit-like to almost rectangular areolae. Internally, areolae occluded by a hymen (Fig. 2E, F).

Figure 2. 

A–G Achnanthidium bratanense sp. nov. (SEM). A, B raphe valve, external view C, D rapheless valve, external view E, F raphe valve, internal view G rapheless valve, internal view. Scale bar: 1 μm.

Holotype (here designated): permanent slide No. MHA 01125, deposited at the Main Botanical Garden, Russian Academy of Sciences (MHA). Fig. 1A illustrates the holotype.

Isotype (here designated): permanent slide No. 01125a, deposited in collection of Maxim Kulikovskiy, Timiryazev Institute of Plant Physiology, Russian Academy of Sciences.

Type locality

Indonesia, Island of Bali, Lake Bratan, 08°16.579'S, 115°09.985'E, leg. I.I. Ivanov on 14 November 2010.

Etymology

The specific epithet refers to the type locality, Lake Bratan.

Ecology

Achnanthidium bratanense together with Gogorevia rinatii were the most abundant species in the sample. Rarely single frustules of Planothidium sp., Stauroneis sp., Cymbella sp. and other diatoms were encountered. During sampling the temperature was recorded as 25.7 °C, pH as 7.82, and conductivity as 22 μS∙cm−1.

Distribution

So far, this species is known from its type locality only.

Discussion

Our new species is closely related to Achnanthidium macrocephalum (Hustedt) Round & Bukhtiyarova, 1996. This taxon was described by Hustedt (1937) as Achnanthes minutissima var. macrocephala from Sumatra. Recently, Wetzel et al. (2019) have re-examined Hustedt’s type material using both LM and SEM. Although the length and breadth of both species overlapped, A. macrocephalum is generally larger than A. bratanense (Table 1). However, the larger valves of A. macrocephalum have distinctly capitate apices. Despite the high abundance of A. bratanense we could not found such valves with capitate apices. Additionally, both taxa differ in striae density: A. macrocephalum has ca. 38 striae in 10 µm (Wetzel et al. 2019), whereas A. bratanense has 41–44 striae in 10 µm (see Table 1). In contrast to A. macrocephalum, the striae of A. bratanense is composed of 1–4 transapically elongated areolae. In A. macrocephalum the striae composed of 1 (smaller valves) or two (rarely 3) areolae (Wetzel et al. 2019). Also A. bratanense has weakly radiate striae throughout the valve whereas in A. macrocephalum the striae become parallel towards the valve ends.

Table 1.

Comparison of morphological characteristics of Achnanthidium bratanense sp. nov. and closely related taxa.

A. bratanense A. macrocephalum A. rosenstockii var. rosenstockii A. rosenstockii var. inareolatum Kolbesia sichuanenis
Valve length, µm 5.0–8.7 7–12 6–14 9.6–15.1 10.8–14.1
Valve width, µm 2.7–3.2 2.5–3.2 3–4 4.2–5.1 3.2–3.7
Valve outline linear-elliptic linear-elliptic with convex margins linear-lanceolate linear-lanceolate linear-lanceolate
Valve apices subcapitate rounded, broadly capitate subcapitate subcapitate broadly capitate
Striae density 41–44 38 27–32 20 22–26
Number of areolae per stria 1–4 1–2(3) 2–4 1 1
Reference This study Wetzel et al. 2019 Krammer and Lange-Bertalot 2004 Krammer and Lange-Bertalot 2004; Yu et al. 2019 Yu et al. 2019

It should be noted that Hustedt (1942) reported Achnanthes minutissima var. macrocephala from Lake Bratan on Bali and suggested that it might be widely distributed in the Indo-Malayan region. Unfortunately, he gave neither description nor images to support the written statement. It is very likely he actually observed A. bratanense instead of A. macrocephalum. Wetzel et al. (2019) supposed that Hustedt (1937) included in his description of A. macrocephalum two morphotypes.

Also A. bratanense is similar to several other taxa including A. rosenstockii (Lange-Bertalot) Lange-Bertalot var. rosenstockii, 2004, A. rosenstockii var. inareolatum Lange-Bertalot, 2004, and Kolbesia sichuanenis P. Yu, Q-M. You & Q-X Wang, 2019 (Table 1). A. rosenstockii var. rosenstockii is slightly wider than A. bratanense and the stria density is lesser (27–32 in 10 µm vs. 41–44 in 10 µm). A. rosenstockii var. inareolatum differs from the type variety in having striae composed of a single macroareola. Probably, this taxon will be better to place in the genus Karayevia Round & Bukhtiyarova emend. Bukhtiyarova, 2006. From both A. rosenstockii var. inareolatum and Kolbesia sichuanenis, A. bratanense differs in stria structure (number of areolae per stria) and stria density. Also, these taxa are significantly larger than A. bratanense (see Table 1).

Traditionally, three morphological groups are recognized within Achnanthidium (e.g. Novais et al. 2015; Karthick et al. 2017; Krahn et al. 2018; Yu et al. 2019): 1) A. minutissimum complex which is characterized by having straight external distal raphe ends, and striae density that increase towards the apex; 2) A. pyrenaicum complex which is characterized by having external distal raphe ends that deflect or hook to one side of the valve, and 3) A. exiguum complex have external distal raphe ends curved in opposite directions. Recently, the latter complex has been segregated into a new genus, Gogorevia (Kulikovskiy et al. 2020a). Although, A. bratanense, A. macrocephalum and A. rosenstockii can be placed in A. minutissimum complex based on the raphe structure, they have completely different striae structure and represent a separate morphological group. Interestingly, Pinseel et al. (2017) revealed 12 distinct lineages within A. minutissimum complex and one of them was described as the new species, A. digitatum Pinseel et al., 2017. Recently, Tseplik et al. (2021b) described from the ancient Lake Matano (island of Sulawesi, Indonesia) the new species, A. gladius Tseplik et al., 2021b, which was closely related to the latter taxon. Thus, further detailed study of the pore apparatus ultrastructure as well as molecular studies of A. bratanense and allied taxa will help to better understand the taxonomic status and phylogenetic placement of this morphological group.

Acknowledgements

Authors are grateful to the staff of the Interlaboratory Centre of Electron Microscopy of the Papanin Institute for Biology of Inland Waters, RAS, for technical assistance. Also, we are grateful to the Subject Editor, Dr. Kalina Manoylov, and two reviewers for their valuable suggestions and corrections. Publication is based on research carried out with financial support by Russian Science Foundation (project No. 19-14-00320) for LM and SEM and by framework of the state assignment (theme No. 121041200194-7) for finishing the manuscript.

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