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Research Article
Thylacopteris minuta (Polypodiaceae), a new fern species from Myanmar
expand article infoKiyotaka Hori, Phyo Kay Khine§, Tao Fujiwara§|, Thant Shin, Harald Schneider§
‡ The Kochi Prefectural Makino Botanical Garden, Kochi, Japan
§ Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Mengla, China
| Tokyo Metropolitan University, Tokyo, Japan
¶ Forest Research Institute, Yezin, Myanmar
Open Access

Abstract

The genus Thylacopteris is a small, phylogenetically isolated genus belonging to the fern family Polypodiaceae. This study describes a new species, Thylacopteris minuta, based on collections obtained during field surveys of Shan State, Myanmar. This new species is distinct from other species of Thylacopteris in its small size and presence of sclerenchyma strands in the rhizome. This species is also distinct from the only other species of Thylacopteris with molecular data available, T. papillosa, in a plastid rbcL phylogeny of Polypodiaceae. This new discovery of Thylacopteris from Myanmar suggests that this genus is still overlooked in Southeast Asia.

Keywords

Myanmar, new species, Polypodiaceae, Thylacopteris

Introduction

With about 1,652 species, Polypodiaceae is the second largest family of pteridophytes (ferns and lycophytes) (PPGI 2016). Species of this family share the presence of some characteristics such as yellowish or greenish spores, round or flat, yellowish-brown, exindusiate sori, and creeping rhizomes usually covered by scales (Zhang et al. 2013). Despite the progress in our understanding of the generic classification of these ferns as summarized in PPGI (2016), some issues are still unresolved. Based on recent molecular studies, Testo et al. (2019) segregated the genus Bosmania Testo, Dendroconche Copel. and Zealandia Testo from the paraphyletic genus Microsorum. Link, and Zhao et al. (2020) expanded the definition of the genus Lepisorus (J.Sm.) Ching. In addition, new species are still being discovered in Polypodiaceae in recent studies (Khine et al. 2016; Zhao et al. 2017) around Southeast Asia. To improve the pteridophyte flora of Southeast Asia, collections and molecular studies of Polypodiaceae are still needed in this area. In this study, we address the relationships of Thylacopteris specimens obtained in Shan State, Myanmar. The genus Thylacopteris includes currently only two species (Rödl-Linder 1994) of which one, Thylacopteris diaphana (Brause) Copel., is endemic to New Guinea. The other species, T. papillosa (Blume) Kunze ex J.Sm, is known to occur throughout Malesia but has not been found in the north of the Isthmus of Kra (Rödl-Linder 1994).

The genus Thylacopteris Kunze ex J.Sm. was established by Smith (1875) with T. papillosa as the type species. This species was originally described as Polypodium pillosum Blume based on an accession collected in Java (Blume 1828). As distinct characteristics of Thylacopteris from Polypodium sensu stricto, Blume (1828) mentioned the deeply sunken sori and the articulation of the lateral segments to the rachis. Subsequently, Copeland (1947) added a second species by introducing the combination T. diaphana (Brause) Copel. based on P. diaphanum Brause, which was based on an accession collected in New Guinea (Brause 1912). Some studies pointed out the close relationships of the genus Thylacopteris with either Goniophlebium or Polypodium (Christensen and Holttum 1934; Ching 1978; Tryon and Tryon 1982). Subsequently, Thylacopteris was treated as a group of Polypodium (Holttum 1966; Tryon and Lugardon 1991), or of uncertain systematic position in Polypodiaceae (Hennipman et al. 1990). Finally, utilizing DNA-based phylogenetics, Thylacopteris was found to be sister to a clade including Goniophlebium, Lepisorus, and Microsorum (Schneider et al. 2004). Considering the reported results of phylogenetic studies focusing on Polypodiacae, Thylacotperis was included in a broadly defined Microsoroideae in PPG I (2016). Reflecting its isolated phylogenetic position, Chen et al. (2019, 2021) introduced a tribe Thylacoptereae only including Thylacopteris.

In the recent years, some studies have reported new species (Khine et al. 2016) or new records of ferns (Nwe et al. 2016; Khine et al. 2017; Hori et al. 2019; Khine and Schneider 2020) from Myanmar. The discovery of new species and new records reflect the lack of comprehensive fern floristic studies of Myanmar (Khine et al. 2017; Khine and Schneider 2020), which limit the ability to report and manage the conservation of Myanmar’s unique biodiversity (Khine and Schneider 2020). Here, we describe a new species of the genus Thylacopteris from Myanmar and the first record of this genus in the country based on morphological characteristics and molecular phylogenetic analysis of Polypodiaceae.

Materials and methods

We collected three specimens in Shan State, Myanmar during the inventories conducted under the leadership of the Makino Botanical Garden team on 13th September 2015, 16th September 2015, and 30th September 2019 together with the team from the Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences. To identify the new species Thylacopteris minuta sp. nov., the following characteristics were studied carefully and compared to the description and specimens of previously described species: the size and shape of plants, morphology of leaves including the shape of segments, stipe, anatomy of rhizome, scales, and the morphology of reproductive organs including sori, sporangia, and spores. Voucher specimens were deposited at the herbarium of the Kochi Prefectural Makino Botanical Garden (MBK), Xishuangbanna Tropical Botanical Garden (HITBC), Queen Sirikit Botanic Garden (QBG) and the Forest Research Institute, Myanmar (RAF). Herbarium codes follow Thiers (2021).

The plastid rbcL gene was employed for phylogenetic analysis. Total DNA was extracted from silica-dried leaves using cetyltrimethylammonium bromide (CTAB) solution according to the method of Doyle and Doyle (1990). PCR amplification was performed using the primers af3 and cr3 (Hori et al. 2018) and PrimeSTAR Max DNA Polymerase (Takara, Kyoto, Japan). PCR involved an initial denaturation step at 95 °C for 10 min, followed by 35 cycles of denaturation, annealing, and elongation steps at 98 °C for 10 s, 55 °C for 5 s, and 72 °C for 8 s, respectively (Model 9700 Thermal Cycler, Applied Biosystems, Foster City, CA, USA). The PCR products were purified using Illustra ExoStar 1-Step (GE Healthcare, Wisconsin, USA) and used as templates for direct sequencing. Reaction mixtures for sequencing were prepared using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, USA). The reaction mixtures were analyzed using an ABI 3130 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA).

To estimate the phylogenetic position of the accession of interest, plastid rbcL sequences of Polypodiaceae were obtained from Genbank (https://www.ncbi.nlm.nih.gov/genbank/), covering all genera accepted in PPG I (2016) as far as data availability enabled. To reflect recent progress in our understanding of the natural classification of Polypodiaceae, the treatment of some taxa deviated from PPGI by adapting new concepts (Testo et al. 2019; Zhao et al. 2020; Chen et al. 2021). In the Genbank database, some accessions contained indels, which should not be present in rbcL since it is a protein-coding gene; we removed such low-quality accessions. The final data set included 94 accessions of Polypodiaceae, three samples of Thylacopteris minuta from Myanmar, and a set of outgroup taxa including Davallia, Oleandra, Nephrolepis and Tectaria (Table 1). The rbcL sequences were aligned using MUSCLE (Edgar 2004) and analyzed with Bayesian inference (BI) using MrBayes 3.2.6 (Ronquist et al. 2012) and maximum likelihood (ML) using MEGA X software (Kumar et al. 2018). Based on BIC values, GTR + G + I model was selected as the best-fit model of sequence evolution for BI analysis by jModelTest 2.1.10 (Darriba et al. 2012), and Tamura 3-parameter + G + I model was selected for the ML analysis by MEGA X software. Four chains of Markov chain Monte Carlo were run simultaneously and sampled every 100 generations for 1 million generations in total. Tracer 1.7.1 (Rambaut et al. 2018) was used to examine the posterior distribution of all parameters and their associated statistics, including estimated sample sizes. The first 2,500 sample trees from each run were discarded as burn-in. In ML analysis, initial trees for the heuristic search were obtained automatically by applying the Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using the Tamura 3-parameter model, and then selecting the topology with superior log likelihood value. A discrete Gamma distribution was used to model evolutionary rate differences among sites (5 categories (+G, parameter = 0.9922)). The rate variation model allowed for some sites to be evolutionarily invariable ([+I], 57.20% sites). The tree was drawn to scale, with branch lengths measured in the number of substitutions per site. The bootstrap method with 1,000 replications was employed in ML analysis.

Table 1.

Accessions of rbcL sequences in this study.

Accessions of rbcL sequences Species
AF468205 Adenophorus montanus
AY529147 Aglaomorpha acuminata
AF470349 Aglaomorpha coronans
AY529150 Aglaomorpha heraclea
MW138159 Alansmia smithii
MT215977 Archigrammitis marquesensis
JQ685380 Arthromeris lehmannii
MG948938 Ascogrammitis anfractuosa
EU482962 Bosmania membranacea
MT215995 Calymmodon cucullatus
MF318061 Campyloneurum brevifolium
MF317971 Campyloneurum lorentzii
MF318013 Campyloneurum rigidum
MW138183 Ceradenia kalbreyeri
KM218797 Chrysogrammitis musgraveana
EF178615 Cochlidium serrulatum
MT657584 Ctenopterella blechnoides
KM218775 Dasygrammitis brevivenosa
MZ957125 Davallia pulchra
KM114198 Davallia solida var. fejeensis
MN018180 Dendroconche annabellae
MN018176 Dendroconche sayeri
DQ227292 Dictymia brownii
DQ164441 Dictymia mckeei
MW138254 Enterosora trifurcata
KM218794 Galactodenia parrisiae
MN017598 Goniophlebium amoenum
AB043100 Goniophlebium formosanum
DQ078627 Goniophlebium microrhizoma
AB043098 Goniophlebium niponicum
MT657640 Goniophlebium percussum
AB043099 Goniophlebium persicifolium
MT657642 Goniophlebium subauriculatum
MT216033 Grammitis cincta
AB232409 Gymnogrammitis dareiformis
AF470322 Lecanopteris carnosa
AF470329 Lecanopteris crustacea
AF470325 Lecanopteris luzonensis
GU387043 Lellingeria dissimulans
MT169815 Lepisorus carnosus
MN623364 Lepisorus hederaceus
MT169813 Lepisorus longifolius
MT169824 Lepisorus normalis
AY362564 Lepisorus nudus
EU482971 Lepisorus superficialis
GQ256304 Lepisorus thunbergianus
GQ256310 Lepisorus uchiyamae
MH768462 Leptochilus decurrens
MH768470 Leptochilus digitatus
MH768471 Leptochilus saxicola
GU376488 Leucotrichum mitchellae
KF992501 Loxogramme lanceolata
DQ227294 Loxogramme salicifolia
GU476898 Melpomene anazalea
MW138194 Microgramma lycopodioides
AY362579 Microgramma squamulosa
MF317960 Microgramma vacciniifolia
AY362344 Micropolypodium hyalinum
LC496693 Microsorum cuspidatum
KY099830 Microsorum membranifolium
DQ179633 Microsorum scolopendria
MW620392 Moranopteris taenifolia
MT216066 Nephrolepis cordifolia
MT216068 Nephrolepis hirsutula
EF463254 Niphidium crassifolium
MF317999 Niphidium longifolium
JQ904094 Notogrammitis billardierei
EF463242 Oleandra articulata
AB232405 Oleandra pistillaris
MT657589 Oreogrammitis forbesiana
EF463255 Pecluma eurybasis
AY362588 Pecluma ptilodon
KT780748 Pecluma sicca
MW138202 Phlebodium pseudoaureum
MN623367 Platycerium bifurcatum
AY362591 Pleopeltis fructuosa
EF463258 Pleopeltis sanctae-rosae
KF909057 Pleurosoriopsis makinoi
KF909059 Polypodium scouleri
KF186527 Polypodium virginianum
AB044899 Polypodium vulgare
MT657600 Prosaptia alata
EF463259 Pyrrosia polydactyla
AY362558 Pyrrosia rupestris
EF463260 Pyrrosia serpens
KM218771 Radiogrammitis holttumii
AY096199 Selliguea feei
AF470347 Selliguea hastata
AY529171 Selliguea laciniata
MW138195 Stenogrammitis limula
DQ168808 Synammia intermedia
KF667652 Tectaria griffithii
EF463274 Tectaria trifoliata
KM218802 Terpsichore aspleniifolia
KM218758 Themelium decrescens
LC685475 Thylacopteris minuta sp.nov., Baba et al. 103191
LC685476 Thylacopteris minuta sp.nov., Baba et al. 103361
LC685054 Thylacopteris minuta sp.nov., Hori et al. 108601
AY459175 Thylacopteris papillosa
MH665089 Thylacopteris papillosa
KM218780 Tomophyllum macrum
MG452028 Zealandia powellii
DQ401117 Zealadia pustulatum
DQ179635 Zealandia vieillardii
KM218793 Zygophlebia devoluta

Results and discussions

The aligned matrix included 1209 bp of rbcL, of which 329 bp (27%) were parsimony-informative. The ML (the highest log likelihood = −11294.22) tree showed that the three accessions of Thylacopteris minuta sp.nov (Baba et al. 103191, 103361, Hori et al. 108601) comprised a clade with two accessions of T. papillosa (Fig. 1). The rbcL sequence of Myanmar accessions of Thylacopteris had 25 substitutions relative to T. papillosa. The phylogenetic placement of T. diaphana is unresolved because DNA sequences were not available in this study. However, at least, morphological characteristics of the new species T. minuta can be differentiated from those of T. diaphana and T. papillosa as described below.

Figure 1. 

Phylogenetic hypothesis selected by applying maximum likelihood (the highest log-likelihood = −11294.22) to rbcL sequences. Posterior probabilities (> 0.90) and bootstrap percentages (> 70%) of Bayesian inference/maximum likelihood analyses are depicted at each node.

Taxonomic treatment

Thylacopteris minuta K.Hori & Khine, sp. nov.

Diagnosis

Thylacopteris minuta is similar to T. papillosa with 20–40 sclerenchyma strands per rhizome in cross-section. However, T. minuta is distinct from T. papillosa with sori shallowly sunken vs. T. papillosa sori deeply sunken. In addition, the lamina of T. minuta has a maximal length of 15 cm vs. a maximal length of 59 cm in T. papillosa. Thylacopteris minuta is distinct from the New Guinea endemic T. diaphana, which lacks sclerenchyma strands in the rhizome, has superficial sori, and lamina with a maximal length of 45 cm.

Type

Myanmar: Shan State; Ah Lel Chaung reserve forest, Ywangan Township. 20°59'44.8"N, 96°34'26.81"E, ca.1325 m, 30 Sep. 2019, K. Hori, P.K. Khine [“Kine”], T. Fujiwara, M. Nagashima, P.P. Shwe & A.K. Moe 108601 (holotype: MBK 0328421 (herbarium barcode), Figs 25 isotype: HITBC, RAF).

Figure 2. 

Thylacopteris minuta K. Hori & P.K.Khine (holotype, Hori et al. 108601 = MBK0328421, illustration by K.Hori) A habit B abaxial view of a middle segment C adaxial view of a middle segment D cross-section of rhizome E spore F rhizome scales G sporangium. Scale bars: 5 cm (A1–A3); 1 cm (B–C); 0.5 mm (D); 10 µm (E); 20 µm (F); 200 µm (G).

Epilithic

Rhizome long-creeping, weakly branched, 1.0–2.0 mm in diam. (without scales), light brown, densely clothed with scales, phyllopodia sometimes prominent, these 1.0–2.0 mm high, 5.0–10.0 mm apart; 20–40 black sclerenchyma strands in rhizome, longitudinal, scattered in the ground tissue. Rhizome scales evenly inserted, dull brown, fragile, adpressed or apically spreading, quite densely set, deciduous, deltoid or ovate, 1.0–1.5 mm long, 0.5–1.0 mm wide, gradually narrowed from base to apex, sometimes with wavy margins, apex acute or rounded. Cell walls of rhizome scales dark brown, jigsaw-puzzle-shaped and wavy at basal and central part of scales, thickened, densely warty, in a single layer or double layers in basal scales. Fronds monomorphic, articulate to rhizome, petiolate. Stipes glabrous, 3.0–5.0 cm long, 0.7–1.0 mm diam, yellowish green. Blades membranous, lanceolate, 7.0–15.0 cm long, 2.0–4.0 cm wide, equally wide all along or rather wider above the basal part, pinnatisect, yellowish green. Segments glabrous, 20–30 pairs, lanceolate, ascending at an angle of 90°, 0.8–2.3 cm long, 0.3–0.5 cm wide, apically obtuse, entire at basal margin, crenate at apical margin, lower segments sometimes reduced, apical segments continuously reduced, terminal segments adnate or caudate. Veins free, once-forked, excurrent with terminal hydathodes. Sori exindusiate, uniserial on each side of costa, placed medially between costa and margin, shallowly sunken, 0.5–1.0 mm in diam., depth of papillae 0.2–0.5 mm, paraphyses absent. Sporangium globe-shaped, long stalked, 200–250 µm in diam., annulus vertical, indurated cells 10–13. Spores bilateral, oblong, light yellow, 40–60 µm long, 25–35 µm wide in lateral view, laesura 20–25 µm long, exospore smooth, perispore thin, surface shallowly wrinkled, globules absent.

Distribution

Myanmar.

Habitat

Epilithic, growing on shady surfaces of limestones (1–3 m high) in semi-evergreen or evergreen forest; altitude 940–1450 m.

Etymology

The name refers to the relatively small size of this species compared to other species of Thylacopteris.

Additional specimens examined

Myanmar: Shan State; Phaya Taung, Lein Le village, Paunglang Reserve Forest, Pinlaung Township; 19°59'41.0"N, 96°39'3.0"E, ca.947 m alt., 13 Sep. 2015, Y. Baba, K. Kertsawang, C. Kilgour, C. Puglisi, M. Rodda, P. Srisan­ga, T. Shin & P.P. Hnin 103191 (MBK0306471, duplicates on RAF, QBG). ibid., road between Nyaung Phyu village and Pinglaung village, Paunglang Reserve Forest, Pinlaung Township; 20°02'56.1"N, 96°46'00.1"E, ca.1448 m alt., 16 Sep. 2015, ibid., 103361 (MBK0313746, duplicates in RAF, QBG).

Notes

The genus Thylacopteris is sometimes confused with Goniophlebium and Polypodium (Rödl-Linder 1994; Fraser-Jenkins 2020). Warty cell walls of rhizome scales (Fig. 3) can be used to conclusively identify the genus Thylacopteris (Rödl-Linder 1994).

Figure 3. 

Warty, thickened cell walls of rhizome scales (MBK 0328421; photo taken using DP20 microscope camera, OLYMPUS, Japan). Scale bar: 2.5 µm.

Figure 4. 

The distribution of Thylacopteris minuta sp.nov.

Figure 5. 

Thylacopteris minuta in its natural habitat (MBK 0328421). Scale bar: 5 cm. (Photo by P.K. Khine).

Key to species of the genus Thylacopteris

1 Blades 7–15 cm long T. minuta
Blades 30–60 cm long 2
2 Sori deeply sunken; sclerenchyma present in cross-section of rhizome T. papillosa
Sori not sunken sclerenchyma; absent in cross-section of rhizome T. diaphana

Acknowledgements

This study was conducted under a Memorandum of Understanding between the Forest Department, Ministry of Natural Resources and Environmental Conservation, Myanmar and the Kochi Prefectural Makino Botanical Garden, Japan. We express our cordial thanks to Dr. Nyi Nyi Kyaw (Director General (retired) of the Forest Department), Dr. Thaung Naing Oo (Deputy Director General of the Forest Department), Dr. Naing Zaw Htun (Director (retired) of the Nature and Wildlife Conservation Division), Myanmar Forest Department and Ministry of Natural Resources and Environmental Conservation, for their help in coordinating the expeditions. We are grateful to the Myanmar-Japanese Cooperative Program for allowing us to study the specimens collected in Shan State. Field work was financially supported through the JICA grassroots program, the Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, and the Post-doctoral orientation training in Yunnan Province in 2018 (Y7YN021B14). This research received partial financial support through the Kochi Prefectural Government. We are grateful to Dr. Thais Almeida, Dr. Weston Testo for reviewing and Dr. Joel Nitta for management of this article.

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