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Research Article
Two new freshwater species of the genus Achnanthidium (Bacillariophyta, Achnanthidiaceae) from Qingxi River, China
expand article infoPan Yu‡§, Qingmin You‡, Wanting Pang‡, Quanxi Wang‡
‡ Shanghai Normal University, Shanghai, China
§ Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China

Corresponding author: Quanxi Wang ( wangqx@shnu.edu.cn )

Academic editor: Wolf-Henning Kusber
© 2022 Pan Yu, Qingmin You, Wanting Pang, Quanxi Wang.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation: Yu P, You Q, Pang W, Wang Q (2022) Two new freshwater species of the genus Achnanthidium (Bacillariophyta, Achnanthidiaceae) from Qingxi River, China. PhytoKeys 192: 11-28. https://doi.org/10.3897/phytokeys.191.78489
Open Access

Abstract

We describe two new Achnanthidium species, A. anhuense sp. nov. and A. qingxiense sp. nov., from Qingxi River, Anhui Province, China, based on light and scanning electron microscopy. Both species belong to the “A. pyrenaicum complex” of the Achnanthidium genus, based on their possession of transapically-elongated areolae and deflected external distal raphe fissures. A. anhuense sp. nov. has a slightly irregular linear-lanceolate valve with rounded or weakly protracted apices and a transapically rectangular or bow tie central area on the raphe valve. A. qingxiense sp. nov. has a linear-lanceolate valve with rounded apices and the axial area distinct expanded apices on the rapheless valve. Both species differ sufficiently from other similar species, based on valve outline, shape of the axial and central areas and striae density. These new species were all collected from stone substratum.

Keywords

Diatoms, monoraphid, morphology, new species, taxonomy

Introduction

The diatom genus Achnanthidium Kützing (1844) was originally described by Kützing, as a subgenus of the Achnanthes Bory de Saint-Vincent (1822). The species Achnanthes microcephalum Kützing was the type species of the subgenus (Pérès et al. 2014; Yu et al. 2019a). In the 1990s, Round et al. (1990) resurrected Achnanthidium and elevated it to the genus level. Later, Round and Bukhtiyarova (1996) redefined the circumscription of this genus, with the main identification characteristics of Achnanthidium including small cells with a length and width of usually less than 30 µm and 5 µm, respectively, linear-lanceolate to lanceolate-elliptic valves and straight or curved external distal raphe ends (Round and Bukhtiyarova 1996; Yu et al. 2019 a, b). Species of Achnanthidium are widely distributed in various types of freshwater habitats in which they are common and abundant (Novais et al. 2011; Pinseel et al. 2015; Karthick et al. 2017; You et al. 2019; Yu et al. 2019a, b).

Owing to the small size (usually less than 30 µm in length and less than 5 µm in breadth) and identification characteristics of Achnanthidium, this genus has been subdivided into three major subgroups. The A. minutissimum complex includes species with straight external distal raphe ends and linear to linear-lanceolate valve shapes, increasing striae density toward the apices and round external areolar openings. The species of the A. pyrenaicum complex have external distal raphe ends that are deflected or hooked to one side of the valve and slit-like areolar openings. The members of the A. exiguum complex have external distal raphe ends curved in opposite directions (Compère and Van de Vijver 2011; Karthick et al. 2017; Yu et al. 2018, 2019a; Miao et al. 2020; Tseplik et al. 2021; You et al. 2021). A. exiguum and its relatives have been segregated into a new genus of Gogorevia Kulikovskiy, Glushchenko, Maltsev and Kociolek (Kulikovskiy et al. 2020).

Presently, the number of species in the genus Achnanthidium is greater than 200 (Marquardt et al. 2017; Kociolek et al. 2018; You et al. 2021). Before the year of 2000, 11 new Achnanthes species had been described from China (Hustedt 1922; Jao 1964; Jao et al. 1974; Qi and Xie 1984; Zhu and Chen 1994, 1996; Liu et al. 2021). Some of these species should be transferred to Achnanthidium, but owing to the loss of the type material, it is difficult to confirm their taxonomic position. It is, therefore, necessary to collect samples from the type locality, and re-evaluate their taxonomic position (Liu et al. 2021). From 2001 to 2021, 14 new Achnanthidium species have been described from China (Liu et al. 2016; Yu et al. 2018, 2019a, b, You et al. 2019, 2021; Liu et al. 2021). During an investigation of the freshwater diatoms from the Qingxi River, two unknown Achnanthidium species were discovered. The purpose of this present study was to document and formally describe those species with light microscopy (LM) and scanning electron microscopy (SEM) and compare the new species with morphologically similar taxa.

Materials and methods

Diatom samples were collected from the Qingxi River located in Chizhou City, Anhui Province, China, in January 2018. In the field, several water chemistry parameters were recorded, including pH, temperature, dissolved oxygen, salinity, total dissolved solids (TDS) and conductivity, using a YSIPro Plus multiparameter meter (YSI, Ohio, USA). Diatom samples were collected from stones using clean toothbrushes and the samples were placed in a bottle and preserved with formalin (4% final concentration). Total phosphorus (TP) was measured by alkaline potassium persulphate digestion ultraviolet spectrophotometry, total nitrogen (TN) was measured by potassium persulphate digestion ammonium molybdate spectrophotometry and chemical oxygen demand (COD) was measured with the potassium permanganate index method (CSEPB 2002).

In the laboratory, diatom samples were cleaned with concentrated nitric acid using the Microwave Accelerated Reaction System (Model MARS, CEM Corporation, Charlotte, USA) (Parr et al. 2004) with a pre-programmed digestion scheme (temperature, 180 °C) (Yu et al. 2017, 2019a, b). Next, samples were alternately centrifuged for 5 min at 3500 rpm (TDZ5-WS, Luyi Corporation, Shanghai, China) and washed six times using distilled water until the pH of the sample was close to neutral. Finally, the cleaned samples were kept in 95% ethanol. Cleaned diatom frustules were mounted in Naphrax for LM or air-dried on to cover slips and mounted on to alloy stubs for observation by SEM. The LM observations were made with an Olympus BX-53 microscope (Tokyo, Japan) fitted with DIC optics and a 100× oil immersion objective (1.4 numerical aperture) and an Olympus DP-71 digital camera. The SEM examination was conducted using a Hitachi SU8010 (1–2 kV, WD 8 mm) (Tokyo, Japan). Images were compiled with Adobe Photoshop CS6. Morphological terminology followed Round et al. (1990). All of the diatom samples and permanent slides are housed in the Lab of Algae and Environment, College of Life Sciences, Shanghai Normal University (SHTU), Shanghai, China.

Results

Achnanthidium anhuense P. Yu, Q. M. You & Q. X. Wang, sp. nov.

Figs 1A–AD, 2–5

Description

LM (Fig. 1A–AD), valves are slightly irregularly linear-lanceolate in shape, with rounded or weakly protracted apices. Valve length 13–35.7 µm, breadth 3.5–4.5 µm (n = 200). Raphe valve is concave, with narrow, linear-lanceolate axial area, with a central area that is transapically rectangular or bow tie-shaped, usually slightly asymmetric. Striae radiate at the middle portion and nearly parallel towards apices, the number of striae is 18–20 in 10 µm at the middle portion, 26–32 in 10 µm near the apices. Rapheless valve is convex, axial area narrow linear-lanceolate and weakly expanded at the middle portion of the valve. Striae are nearly parallel, 16–26 in 10 µm in the centre and 22–30 in 10 µm near the apices.

Figures 1. 

A–AD LM valve views of Achnanthidium anhuense sp. nov. AE–AS LM valve views of Achnanthidium qingxiense sp. nov. Scale bar: 10 µm.

In the SEM, both valves have a narrow hyaline area at the valve face and mantle junction (Figs 2A, B, 4A, B). Raphe valve: Externally, the raphe is filiform and straight (Fig. 2A, B), distal raphe ends are deflected to the same side (Fig. 2A–C, E), and proximal raphe ends straight and teardrop-shaped (Fig. 2A, B, D, F). Striae uniseriate, containing 3–6 round, oval or transapically-orientated areolae in the middle portion of the valve and 1–3 round, oval or transapically-orientated areolae at the apices (Fig. 2A–C, E). Valve mantle with a single row of linear areolae extend along the valve, but with a slight interruption in the apices (Fig. 2B, C, E). Internally, raphe terminates in raised helictoglossae close to the apices (Fig. 3A–D), proximal raphe ends form small hooks and are distinctly deflected in opposite directions (Fig. 3A, B, E). Areolae transapically elongated in the central portion of the valve, becoming larger and oblong at the apices (Fig. 3C–E). Areolae are occluded by hymenes perforated by delicate slits and each hymene joins with the adjacent hymene (Fig. 3F).

Figures 2. 

A–F Achnanthidium anhuense sp. nov., SEM external views of raphe valve A, B external view of an entire raphe valve C, E apices of the valve, showing the distal raphe ends D, F central area of the valve, showing the proximal raphe ends, D seems detail of A and F of B. Scale bars: 5 µm (A, B); 1 µm (C–F).

Figures 3. 

A–F Achnanthidium anhuense sp. nov., SEM internal views of raphe valve A, B internal view of an entire raphe valve C, D apices of the valve, showing the distal raphe ends E central area of the valve, showing the proximal raphe ends F internal areola openings with fine hymenate structures. Scale bars: 5 µm (A, B); 1 µm (E); 0.5 µm (C, D); 0.3 µm (F).

Rapheless valve: Externally, the axial area is linear, being weakly expanded in the central area (Fig. 4A, B). Striae are uniseriate, comprise of 4–6 round or transapically elongated areolae in the central area (Fig. 4A, B, D) and 1–4 round or irregular oblong areolae at the apices (Fig. 4A–C). A row of slit-like areolae is present on the mantle (Fig. 4C, D). Internally, axial area slightly raised, with a shallow cutting line at the middle portion (Fig. 5A, B, E) and have a shallow depression at the ends of the axial area (Fig. 5A–D). Areolae are transapically oval in the centre of the valve (Fig. 5A, B, E) and large, irregular and oblong at the ends (Fig. 5A–D). Areolae are occluded by hymenes perforated by delicate slits and each hymene joins with the adjacent hymene (Fig. 5F).

Figures 4. 

A–D Achnanthidium anhuense sp. nov., SEM external views of rapheless valve A, B external view of an entire rapheless valve C apices of the valve D central area of the valve. Scale bars: 5 µm (A, B); 1 µm (C, D).

Figures 5. 

A–F Achnanthidium anhuense sp. nov., SEM internal views of rapheless valve A, B internal view of an entire rapheless valve C, D apices of the valve E central area of the valve F internal areola openings with fine hymenate structures. Scale bars: 5 µm (A, B); 1 µm (E); 0.5 µm (C, D, F).

Holotype

(designated here). SHTU! Slide QXH201801-Z7 in Lab of Algae and Environment, College of Life Sciences, Shanghai Normal University, Shanghai, China. Holotype illustrated in Fig. 1H, W.

Registration

http://phycobank.org/103057

Type locality

China. Qingxi River, Anhui Province, 30°14'39"N, 117°49'58"E, leg. Q.X. Wang and P. Yu, 23th January 2018.

Etymology

The species is named for the place where it was found, namely Anhui Province.

Ecology

Collected in one sample (QXH201801-Z7) on stone. Water temperature – 8.1 °C, pH – 7.8, Salinity – 0.05‰, TDS – 95.55 mg·l-1, EC – 99.3 μS·cm–1, TN – 0.5 mg·l-1, TP – 0.03 mg·l-1, COD – 0.1 mg·l-1.

Distribution

So far only known from the type locality.

Achnanthidium qingxiense Q. M. You, P. Yu & Q. X. Wang, sp. nov.

Figs 1AE–AS, 6–9

Description

LM (Fig. 1AE–AS). Valves linear-lanceolate in shape, with rounded or weakly protracted apices. The valve length is 22.5–28 µm and breadth of 3.8–4.6 µm (n = 30). Raphe valve with a narrow, linear-lanceolate axial area is weakly expanded at the middle portion of the valve. Striae slightly radiate at the centre area, becoming denser towards the apices, 21–25 in 10 µm at the centre, 42–44 in 10 µm near the apices. Rapheless valve with narrow, linear axial area is weakly expanded at the middle portion of the valve. Striae are nearly parallel, becoming denser towards the apices, 20–24 in 10 µm at the centre, up to 32–34 in 10 µm at the apices.

In the SEM, on both valves, the valve mantle has a single row of slit-like areolae (Figs 6B, C, 8A, B). Externally, the raphe is filiform, slightly undulate and has distal raphe ends deflected to the same side (Fig. 6A). On the side of the deflection, there is a depression near the distal raphe ends (Fig. 6A, B). The proximal raphe ends are straight and teardrop-shaped (Fig. 6A, C). Areolae are small, round to transapically orientated, the uniseriate striae are composed of 5–8 areolae in the middle portion of the valve (Fig. 6A, C) and 1–5 areolae at the apex (Fig. 6A, B). Internally, distal raphe ends terminate in raised helictoglossae (Fig. 7A–C), while the proximal raphe ends are weakly deflected in opposite directions (Fig. 7A, B, E). Areolae transapically elongated in the central portion of the valve and becoming larger and oblong at the apices (Fig. 7A–C, E).

Figures 6. 

A–C Achnanthidium qingxiense sp. nov., SEM external views of raphe valve A external view of an entire raphe valve B apices of the valve, showing the distal raphe ends C central area of the valve, showing the proximal raphe ends. Scale bars: 5 µm (A); 1 µm (B, C).

Figures 7. 

A–E Achnanthidium qingxiense sp. nov., SEM internal views of raphe valve A, B internal view of an entire raphe valve C apices of the valve, showing the distal raphe ends E central area of the valve, showing the proximal raphe ends D internal areola openings with fine hymenate structures. Scale bars 5 µm (A, B); 1 µm (C, E); 0.3 µm (D).

Rapheless valve: Externally, the axial area is linear, being weakly expanded at the central area and distinctly expanded at the apices (Fig. 8A–E). Striae are uniseriate, comprise of 5–10 round or transapically orientated areolae in the central area (Fig. 8A, B, E) and 1–4 round, oblong or slit-like areolae at the apices (Fig. 8A–D). Internally, the axial area is slightly raised, with a subtriangular area at the ends of the axial area (Fig. 9A–D). Areolae are transapically oval in the centre of the valve (Fig. 9A, B, E) and large, irregular and oblong at the ends (Fig. 9A–D). On both interiors of both valves, areolae are occluded by hymenes perforated by delicate slits and each hymene joins with the adjacent hymene (Figs 7D, 9F).

Figures 8. 

A–E Achnanthidium qingxiense sp. nov., SEM external views of rapheless valve A, B external view of an entire rapheless valve C, D apices of the valve E central area of the valve. Scale bars: 5 µm (A, B); 1 µm (E); 0.5 µm (C, D).

Figures 9. 

A–F Achnanthidium qingxiense sp. nov., SEM internal views of rapheless valve A, B internal view of an entire rapheless valve C, D apices of the valve E central area of the valve F internal areola openings with fine hymenate structures. Scale bars: 5 µm (A, B); 1 µm (E); 0.5 µm (C, D); 0.3 µm (F).

Holotype

(designated here). SHTU! Slide QXH201801-Z7 in Lab of Algae and Environment, College of Life Sciences, Shanghai Normal University, Shanghai, China. Holotype illustrated in Fig. 1AJ, AO.

Registration

http://phycobank.org/103058

Type locality

China. Qingxi River, Anhui Province, 30°14'39"N, 117°49'58"E, leg. Q.X. Wang and P. Yu, 23th January 2018.

Etymology

The species is named after Qingxi River, where it was discovered.

Ecology

Collected in one sample (QXH201801-Z7) on stone. The environmental conditions are exactly the same as for the A. dubium sp. nov.

Distribution

The new species is known only from the type locality.

Discussion

Based on the generic description of Achnanthidium by Round et al. (1990) and Round and Bukhtiyarova (1996), A. anhuense sp. nov. and A. qingxiense sp. nov. clearly belong to this genus. The two new species possess characters that support their assignment to the A. pyrenaicum complex, deflected external distal raphe fissures (Yu et al. 2018, 2019 b, You et al. 2019, 2021).

Achnanthidium anhuense sp. nov. is similar to a few species, including A. pyrenaicum (Hustedt) Kobayasi (Kobayasi 1997), A. pseudoconspicuum (Foged) Jüttner & Cox (Jüttner, Cox 2011), A. rostropyrenaicum Jüttner & Cox (Jüttner et al. 2011) and A. initium Karthick, Taylor and Hamilton (Karthick et al. 2017). To facilitate a comparison between A. anhuense sp. nov. and these similar species, their morphological characteristics are summarised in Table 1. There is a difference in the valve outline between A. anhuense sp. nov. and these other species, with the valves of A. anhuense being slightly irregularly linear-lanceolate, whereas the values of A. pseudoconspicuum are linear-elliptical and those of A. initium are linear-lanceolate to lanceolate. On the raphe valve, A. anhuense sp. nov. has a transapically rectangular or bow-tie central area, whereas the central area of A. pseudoconspicuum and A. pyrenaicum is small and hardly differentiated. A. pseudoconspicuum has narrow transverse fascia and A. initium has an asymmetrical transverse fascia central area. In addition, the axial area of A. anhuense sp. nov. is linear-lanceolate, but narrow linear in other similar species. The valves of A. anhuense sp. nov. are longer (13–35.7 µm) than in other similar species and wider (3.5–4.5 µm) than in A. initium (3.1–3.6 µm). Moreover, on the raphe valve, the striae density at the middle and apices of A. anhuense sp. nov. is less than in other similar species and, on the rapheless valve, the striae density at the middle of A. anhuense sp. nov. is less (16–26/10 µm) than in A. initium (32–35/10 µm). The striae density at the apices of A. anhuense sp. nov. is less (22–30/10 µm) than in A. initium (32–35/10 µm) and A. pyrenaicum (32–38/10 µm), but higher than in A. pseudoconspicuum (20–24/10 µm).

Table 1.
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Comparison of morphological characteristics of Achnanthidium anhuensis sp. nov. and closely related taxa.

Species/Feature A. anhuensis sp. nov. A. initium Karthick, Taylor & Hamilton A. rostropyrenaicum Jüttner & Cox A. pseudoconspicuum (Foged) Jüttner & Cox A. pyrenaicum (Hustedt) Kobayasi
Valve length (μm) 13–35.7 11–25.2 18–24.5 12.9–21 10–16
Valve width (μm) 3.5–4.5 3.1–3.6 4.3–4.5 2.9–4.8 2.5–4
Valve outline Linear-lanceolate Linear-lanceolate to lanceolate Linear-lanceolate Linear-elliptical Linear-lanceolate
Valve apices Rounded or weakly protracted Rounded to weakly rostrate rounded Rostrate Rounded or slightly cuneate Slightly drawn–out ends
Raphe valve
Axial area Narrow, linear-lanceolate Narrow linear Narrow linear Narrow linear Narrow linear
Central area Rectangular or bow tie Asymmet-rical transverse fascia Small and hardly differentiated Narrow transverse fascia Small and hardly differentiated
Raphe Distal fissures deflected to the same side, internally proximal raphe fissures distinct deflected in opposite direction Distal fissures deflected to the opposite side at an angle of 80–90°, internally proximal ends curved in opposite directions Raphe distal raphe ends curved to the same side, internally proximal raphe endings curved towards opposite side Distal fissures deflected to the opposite side at an angle of 60–80°, internally central raphe ends curve to opposite sides Raphe distal raphe ends curved to the same side, internally proximal raphe endings curved towards opposite side
Density of striae (10 μm) 18–20 (middle), 26–31 (apices) 29–34 20–22 (middle), 32 (apices) 22–24 (middle), 32 (apices) 20–25 (middle), 34–40 (apices)
Number of areolae per stria 3–6 (middle), 1–3 (apices) 2–5 (middle), 1–3 (apices) 2–6 (middle), 1–3 (apices) 3–5 (middle), 1–3 (apices) No data
Rapheless valve
Axial area Narrow linear-lanceolate Narrow linear Narrow linear Narrow linear Narrow linear
Central area Absent Weakly elliptical to almost absent Absent Absent Absent
Density of striae (10 μm) 16–26 (middle), 22–30 (apices) 32–35 22 (middle), 28 (apices) 20–24 20–28 (middle), 32–38 (apices)
Number of areolae per stria 4–6 (middle), 1–2 (apices) 3–5 (middle), 1–3 (apices) 4–6 (middle), 1–2 (apices) 4–6 (middle), 2–3 (apices) No data
References Current study Karthick et al. (2017) Jüttner et al. (2011) Jüttner and Cox (2011) Kobayashi (1997)

Species similar to A. qingxiense sp. nov. include A. gracillimum (Meister) Mayama (Kobayasi et al. 2006), A. chitrakootense Wojtal (Wojtal et al. 2010), A. sinense Liu & Blanco (Liu et al. 2016), A. linannulumm Karthick, Taylor & Hamilton (Karthick et al. 2017) and A. sublanceolatum Yu, You & Wang (Yu et al. 2019a). This group of species is compared in Table 2. Externally, on the raphe valve, A. qingxiense sp. nov. has a linear-lanceolate valve with rounded apices, while A. linannulumm possesses linear elliptical to lanceolate valves and rounded or slightly protracted apices. The species of A. sinense are not protracted, but are acute and round, A. gracillimum has an elliptical to lanceolate valve and narrowly rostrate to subcapitate apices and A. chitrakootense possesses linear to linear-elliptical valve and subcapitate to rounded apices. In addition, A. qingxiense sp. nov. has a narrow linear-lanceolate axial area, whereas the axial area of A. gracillimum is linear and that of A. linannulumm is lanceolate. A. qingxiense sp. nov. has absent central area, while A. sinense possesses a rhombic-lanceolate central area and A. gracillimum has an asymmetrical central area. Moreover, on the rapheless valve, A. qingxiense sp. nov. can easily be separated from other similar species, based on its being distinctly expanded at the apices on the axial area (Figs 8, 9). Conversely, on the raphe valve, the striae density at the middle of A. qingxiense sp. nov. is less (21–25/10 µm) than in A. chitrakootense (26–30/10 µm), but higher than in A. gracillimum (22/10 µm) and the striae density at the apices of A. qingxiense sp. nov. is higher than other similar species. On the rapheless valve, the striae density at the middle of A. qingxiense sp. nov. is less (20–24/10 µm) than in A. linannulumm (24–26/10 µm) and A. chitrakootense (26–30/10 µm), but higher than in A. gracillimum (22/10 µm) and the striae density at the apices of A. qingxiense sp. nov. is higher than other similar species.

Table 2.
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Comparison of morphological characteristics of Achnanthidium qingxiensis sp. nov. and closely related taxa.

Species/Feature A. qingxiensis sp. nov. A. sublanceolatum Yu, You & Wang A. linannulumm Karthick, Taylor & Hamilton A. sinense Liu & Blanco A. gracillimum (Meister) Mayama A. chitrakootense Wojtal
Valve length (μm) 22.5–28 18–35 15.5–32.5 17.5–31.7 19–31.5 13–42
Valve width (μm) 3.8–4.6 4–4.5 2.5–4.5 4.1–6.0 3–4 3.4–4.2
Valve outline Linear-lanceolate Linear-lanceolate Linear elliptical to lanceolate Narrow lanceolate Elliptical to lanceolate Linear to linear-elliptical
Valve apices Rounded Rounded or weakly protracted Rounded or slightly protacted Not protracted, acute round Narrowly rostrate to subcapitate Subcapitate to rounded
Raphe valve
Axial area Narrow, linear-lanceolate Narrow, linear-lanceolate Lanceolate Narrow lanceolate Linear linear-lanceolate
Central area Absent Absent Indistinct to weakly expanded Rhombic-lanceolate Asymmetrical Indistinct to weakly expanded
Raphe Slightly undulate, distal raphe ends deflected towards the same side, internally proximal raphe endings weakly deflected in opposite directions Distal raphe fissures deflected to the same side, internally proximal raphe fissures weakly deflected in opposite direction Distal fissures deflected to the same side at an ange of 80–90°, internally proximal ends curved in opposite directions Raphe distal raphe ends curved to the same side, internally proximal raphe endings curved towards opposite side Distal raphe fissures are sharply bent, internally central raphe ends curve to opposite sides Distal raphe ends strongly curved to the same side, proximal raphe ends with slightly defl ected to opposite sides
Density of striae (10 μm) 21–25 (middle), 42–44 (apices) 20–23 (middle), 34–42 (apices) 24–27 (middle), 32–34 (apices) 21–28 (middle), 40 (apices) 22 (middle), 36 (apices) 26–30
Number of areolae per stria 5–8 (middle), 1–3 (apices) 3–4 (middle), 1–2 (apices) 2–3 5–6 (middle), 1–3 (apices) 4–5 (middle), 1–2 (apices) 2–4 (middle), 1–3 (apices)
Rapheless valve
Axial area Linear Narrow linear-lanceolate Narrow lanceolate Linear Linear Linear
Central area Absent Absent Weakly expanded to absent Absent Weakly expanded to absent Absent
Density of striae (10 μm) 20–24 (middle), 32–34 (apices) 21–24 (middle), 30–36 (apices) 24–26 (middle), 28–30 (apices) 21–27 (middle), 34 (apices) 22 (middle), 36 (apices) 26–30
Number of areolae per stria 5–10 (middle), 1–4 (apices) 2–5 (middle), 1–2 (apices) 2–4 6–7 (middle), 1–3 (apices) 4–5 (middle), 1–3 (apices) 4–5 (middle), 2–4 (apices)
References Current study Yu et al. (2019a) Karthick et al. (2017) Liu et al. (2016) Kobayasi et al. (2006) Wojtal et al. (2010)

A. anhuense sp. nov. and A. qingxiense sp. nov. were collected from stones in Qingxi River, which is fast-flowing. Achnanthidium species can occur across a broad range of trophic conditions, from oligotrophic to eutrophic waters (Karthick et al. 2017; Miao et al. 2020). The A. pyrenaicum complex is abundant in clear and fast- flowing streams and they can be good indicators of specific environmental conditions in freshwater ecosystems (Cantonati and Spitale 2009; Jüttner et al. 2011), which is supported by the results of this study. The two new species occur in low TN (0.5 mg·l-1), TP (0.03 mg·l-1) and COD (0.1 mg·l-1) environments. Moreover, in the type locality, some other monoraphid species co-occur with these new species. The co-occurring monoraphid taxa include A. latecephalum Kobayasi (Kobayasi 1997), A. pyrenaicum (Hustedt) Kobayasi (Kobayasi 1997), Planothidium lanceolatum (Brébisson ex Kützing) Lange-Bertalot (Lange-Bertalot 1999), A. rivulare Potapova & Ponader (Potapova and Ponader 2004) and A. subhudsonis var. kraeuselii (Cholnoky) Cantonati & Lange-Bertalot (Kusber et al. 2017). In further studies, we will continue to study the relationship between diatom diversity and ecology from this region.

Acknowledgements

This research was funded and supported by Biodiversity Survey and Assessment Project of the Ministry of Ecology and Environment, China (No. 2019HJ2096001006), National Natural Science Foundation of China (No. 32100165, 32170205, 31770222) and the Natural Science Foundation of Shanghai (21ZR1447300). We would like to thank Dr. Wanting Pang, Yue Cao and Lixuan Zhang for help in the field and in the preparation of samples.

References

  • Bory de Saint-Vincent JBGN (1822) Achnanthe. Achnanthes. Dictionnaire Classique d’Histoire Naturelle 1: 79–80.
  • Cantonati M, Spitale D (2009) The role of environmental variables in structuring epiphytic and epilithic diatom assemblages in spring and streams of the Dolomiti Bellunesi National Park (south-eastern Alps). Fundamental and Applied Limnology 174(2): 117–133. https://doi.org/10.1127/1863-9135/2009/0174-0117
  • CSEPB [Chinese State Environment Protection Bureau] (2002) Water and wastewater monitoring analysis methods, 4nd edn. Chinese Environment Science Press, Beijing, China.
  • Compère P, Van de Vijver B (2011) Achnanthidium ennediense (Compère) Compère et Van de Vijver comb. nov. (Bacillariophyceae), the true identity of Navicula ennediensis compère from the Ennedi Mountains (Republic of Chad). Algological Studies 136/137: 5–17. https://doi.org/10.1127/1864-1318/2011/0136-0005
  • Hustedt F (1922) Bacillariales aus Innerasien. Gesammelt von Dr. Sven Hedin. In: Hedin S (Ed.) Southern Tibet, discoveries in former times compared with my own researches in 1906–1908. Lithographic Institute of the General Staff of the Swedish Army, Stockholm 6(3), 107–152. https://doi.org/10.5962/bhl.title.64226
  • Jao C (1964) Some fresh-water algae from southern Tibet. Oceanologia et Limnologia Sinica 6(2): 169–192.
  • Jao C, Zhu H, Lee Y (1974) The fresh-water algae from Mount Qomolangma District (in Tibet). Report of the Scientific Survey of Mount Qomolangma District 1966–1968: 92–126.
  • Jüttner I, Cox EJ (2011) Achnanthidium pseudoconspicum comb. nov.: Morphology and ecology of the species and a comparison with related taxa. Diatom Research 26(1): 21–28. https://doi.org/10.1080/0269249X.2011.573707
  • Jüttner I, Chimonides J, Cox J (2011) Morphology, ecology and biogeography of diatom species related to Achnanthidium pyrenaicum (Hustedt) Kobayasi (Bacillariophyceae) in streams of the Indian and Nepalese Himalaya. Algological Studies 136/137: 45–76. https://doi.org/10.1127/1864-1318/2011/0136-0045
  • Karthick B, Taylor JC, Hamilton PB (2017) Two new species of Achnanthidium Kützing (Bacillariophyceae) from Kolli Hills, Eastern Ghats, India. Fottea 17(1): 65–77. https://doi.org/10.5507/fot.2016.020
  • Kobayasi H, Idei M, Mayama S, Nagumo T, Osada K (2006) H. Kobayasi’s Atlas of Japanese Diatoms based on electron microscopy. Uchida Rokakuho Publishing Co. , Ltd, Tokyo, 531 pp.
  • Kociolek JP, Balasubramanian K, Blanco S, Coste M, Ector L, Liu Y, Kulikovskiy M, Lundholm N, Ludwig T, Potapova M, Rimet F, Sabbe K, Sala S, Sar E, Taylor J, Van de Vijver B, Wetzel CE, Williams DM, Witkowski A, Witkowski J (2018) In DiatomBase. http://www.diatombase.org [accessed on 2018-03-15]
  • Kulikovskiy MS, Maltsev YI, Glushchenko AM, Kuznetsova IV, Kapustin DA, Lange-Bertalot H, Genkal SI, Kociolek JP (2020) Gogorevia, a new monoraphid diatom genus for Achnanthes exigua and allied taxa (Achnanthidiaceae) described on the basis of an integrated molecular and morphological approach. Journal of Phycology 56(6): 1601–1613. https://doi.org/10.1111/jpy.13064
  • Kusber WH, Cantonati M, Lange-Bertalot H (2017) Validation of five diatom novelties published in “Freshwater Benthic Diatoms of Central Europe” and taxonomic treatment of the neglected species Tryblionella hantzschiana. Phytotaxa 328(1): 90–94. https://doi.org/10.11646/phytotaxa.328.1.6
  • Lange-Bertalot H (1999) Neue Kombinationen von Taxa aus Achnanthes Bory (sensu lato). Iconographia Diatomologica 6: 270–283.
  • Liu B, Blanco S, Long H, Jingjing XU, Jiang X (2016) Achnanthidium sinense sp. nov. (Bacillariophyta) from the Wuling Mountains Area, China. Phytotaxa 284(3): 194–202. https://doi.org/10.11646/phytotaxa.284.3.4
  • Liu Y, Tan X, Kociolek JP, Kulikovskiy M, Lu XX, Fan YW (2021) One new species of Achnanthidium Kützing (Bacillariophyta, Achnanthidiaceae) from the upper Han River, China. Phytotaxa 516(2): 187–194. https://doi.org/10.11646/phytotaxa.516.2.6
  • Marquardt GC, Costa LF, Bicudo DC, Bicudo CEDM, Blanco S, Wetzel CE, Ector L (2017) Type analysis of Achnanthidium minutissimum and A. catenatum and description of A. tropicocatenatum sp. nov. (Bacillariophyta), a common species in Brazilian reservoirs. Plant Ecology and Evolution 150(3): 313–330. https://doi.org/10.5091/plecevo.2017.1325
  • Miao M, Li Z, Hwang EA, Kim HK, Lee H, Kim BH (2020) Two new benthic diatoms of the genus Achnanthidium (Bacillariophyceae) from the Hangang River, Korea. Diversity (Basel) 12(7): e285. https://doi.org/10.3390/d12070285
  • Novais MH, Hlúbiková D, Morais M, Hoffmann L, Ector L (2011) Morphology and ecology of Achnanthidium caravelense (Bacillariophyceae), a new species from Portuguese rivers. Algological Studies 136: 131–150. https://doi.org/10.1127/1864-1318/2011/0136-0131
  • Pérès F, Cohu RL, Delmont D (2014) Achnanthidium barbei sp. nov. and Achnanthidium costei sp. nov., two new diatom species from French rivers. Diatom Research 29(4): 387–397. https://doi.org/10.1080/0269249X.2014.890956
  • Pinseel E, Van de Vijver B, Kopalova K (2015) Achnanthidium petuniabuktianum sp. nov. (Achnanthidiaceae, Bacillariophyta), a new representative of the A. pyrenaicum group from Spitsbergen (Svalbard Archipelago, High Arctic). Phytotaxa 226(1): 63–74. https://doi.org/10.11646/phytotaxa.226.1.6
  • Potapova MG, Ponader KC (2004) Two common North American diatoms, Achnanthidium rivulare sp. nov. and A. deflexum (Reimer) Kingston: Morphology, ecology and comparison with related species. Diatom Research 19(1): 33–57. https://doi.org/10.1080/0269249X.2004.9705606
  • Qi YZ, Xie SQ (1984) The diatom in moss swamp from Hubei Shennongjia. Jinan Yili Xuebao 1984(3): 86–92.
  • Round FE, Crawford RM, Mann DG (1990) The Diatoms. Biology and morphology of the genera. Cambridge University Press, Cambridge, 747 pp.
  • Tseplik ND, Maltsev YI, Glushchenko AM, Kuznetsova IV, Genkal SI, Kociolek JP, Kulikovskiy MS (2021) Achnanthidium tinea sp. nov.-a new monoraphid diatom (Bacillariophyceae) species, described on the basis of molecular and morphological approaches. PhytoKeys 174: 147–163. https://doi.org/10.3897/phytokeys.174.60337
  • Wojtal AZ, Lange-Bertalot H, Nautiyal R, Verma J, Nautiyal P (2010) Achnanthidium chitrakootense spec. nov. from rivers of northern and central India. Polish Botanical Journal 55: 55–64.
  • You QM, Cao Y, Yu P, Kociolek JP, Zhang LX, Wu B, Lowe R, Wang QX (2019) Three new subaerial Achnanthidium (Bacillariophyta) species from a karst landform in the Guizhou Province, China. Fottea 19(2): 138–150. https://doi.org/10.5507/fot.2019.005
  • You QM, Zhao K, Wang YL, Yu P, Kociolek JP, Pang WT, Wang QX (2021) Four new species of monoraphid diatoms from Western Sichuan Plateau in China. Phytotaxa 479(3): 257–274. https://doi.org/10.11646/phytotaxa.479.3.3
  • Yu P, You QM, Kociolek JP, Lowe R, Wang QX (2017) Nupela major sp. nov. a new diatom species from Maolan Nature Reserve, central-south of China. Phytotaxa 311(3): 245–254. https://doi.org/10.11646/phytotaxa.311.3.4
  • Yu P, Kociolek JP, You QM, Wang QX (2018) Achnanthidium longissima sp. nov. (Bacillariophyta), a new diatom species from Jiuzhai Valley, southwestern China. Diatom Research 33(3): 339–348. https://doi.org/10.1080/0269249X.2018.1545704
  • Yu P, You Q, Kociolek JP, Wang Q (2019a) Three new freshwater species of the genus Achnanthidium (Bacillariophyta, Achnanthidiaceae) from Taiping Lake, China. Fottea 19(1): 33–49. https://doi.org/10.5507/fot.2018.015
  • Yu P, You QM, Pang WT, Cao Y, Wang QX (2019b) Five new Achnanthidiaceae species (Bacillariophyta) from Jiuzhai Valley, Sichuan Province, southwestern China. Phytotaxa 405(3): 147–170. https://doi.org/10.11646/phytotaxa.405.3.5
  • Zhu HZ, Chen JY (1994) Study on the diatoms of the Wuling Mountain Region. Compilation of reports on the survey of algal resources, 405 pp.
  • Zhu HZ, Chen JY (1996) New taxa of diatom (Bacillariophyta) from Xizang (Tibet). (II). Zhiwu Fenlei Xuebao 34(1): 102–104.
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