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Research Article
Goodyera medogensis (Orchidaceae), a new species from Tibet, China
expand article infoYi-Hua Tong§, Mei Sun|, Bing-Mou Wang, Huai-Zhen Tian|
‡ South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China
§ Core Botanical Gardens, Chinese Academy of Sciences, Guangzhou, China
| East China Normal University, Shanghai, China
¶ Panyu Central Hospital, Guangzhou, China
Open Access

Abstract

A new species of Goodyera (Orchidaceae) from Tibet, China, G. medogensis, is described and illustrated here. Molecular phylogenetic results based on one nuclear (ITS) and two plastid markers (matK and trnL-F) support the recognition of G. medogensis as a new species in Goodyera subsection Reticulum. Morphologically, the novelty is most similar to G. biflora, G. vittata and especially to G. hemsleyana, but differs by the thick grid lines of the reticulations with a diffused margin on the adaxial surface of the leaf blades, the inflorescence with more flowers, the obliquely obovate-rhombic petals, the yellow or yellowish labellum without a lamella on the blade, and the shorter trichomes on the floral bracts, sepals and ovary. Finally, a key to the species of Goodyera subsect. Reticulum in China is also provided.

Keywords

Cranichideae, Jewel orchid, morphology, new species, Orchidoideae, phylogeny

Introduction

The genus Goodyera R. Br. (Orchidaceae) belongs to the subtribe Goodyerinae (Pridgeon et al. 2003; Chase et al. 2015), which includes three major subdivisions: the Pachyplectron clade, the Goodyera clade and the Cheirosylis clade (Chen et al. 2019). Different phylogenetic studies have revealed that Goodyera is polyphyletic (Juswara 2010; Hu et al. 2016; Chen et al. 2019), which has led Pace (2020) to subsequently propose a new generic arrangement for the Goodyera clade including 11 genera: Goodyera s.s., Cionisaccus Breda, Aspidogyne Garay, Microchilus C. Presl, Kreodanthus Garay, Lepidogyne Blume, Hylophila Lindl., Platylepis A. Rich., Eucosia Blume, Erythrodes Blume, Salacistis Rchb. f. and Paorchis M. C. Pace. Since the available molecular phylogenetic results are based on few markers, and the quality and representativeness of samples are limited, in this study, we still accept a broad concept of Goodyera.

Goodyera s.l. consists of about 99 species distributed in Africa (Mozambique), Western Indian Ocean Islands, Asia, Southwest Pacific islands, Northeast Australia, Europe, Macaronesia (Madeira), North and Central America and the Caribbean (Chen et al. 2009; Zhou et al. 2020; Govaerts et al. 2021; Thiv et al. 2021). It is characterized by an elongate creeping rhizome, a cymbiform lip with a concave-saccate hypochile, sectile pollinia and one stigma.

There are 36 accepted species of Goodyera in China, of which 15 species are endemic (Chen et al. 2009; Guan et al. 2014; Jin and Yang 2015; Zhou et al. 2016; Liu et al. 2019; Wang et al. 2020; Zhou et al. 2020), and only G. repens (L.) R. Br. and G. brachystegia Hand.-Mazz. are distributed to the north of the Yangtze River. During a botanical survey in Medog County, Tibet Autonomous Region in January 2021, a few living plants with greenish reticulated leaves, resembling those of Macodes (Blume) Lindl., were collected and cultivated in Kunming Botanical Garden. Plants bloomed vigorously with many flowers in August. We also found flowering individuals in the field at the same time. The novelty is similar to G. hemsleyana King & Pantl. at first glance. However, it has greenish flowers with a yellow lip, while G. hemsleyana has pink flowers with a white lip. Besides, the reticulations on the leaf blades of the two species are also different.

Based on molecular phylogenetic evidence and a detailed examination of the morphological characters of our materials, the relevant types and a comprehensive array of additional herbarium specimens of similar species, we concluded that the plants discovered in Medog County in January 2021 represented a new species to science. It is described and illustrated here as G. medogensis H. Z. Tian, Y. H. Tong & B. M. Wang and included in a key to the species of Goodyera subsect. Reticulum in China.

Materials and methods

Based on our field observations of Goodyera in China in the past ten years, we identified three species that were closely related to the novelty, viz. G. hemleyana, G. biflora (Lindl.) Hook. f. and G. vittata Benth. ex Hook. f. (Fig. 1). Accordingly, we checked the types and additional material of these three taxa together with other pertinent specimens at HSNU, IBSC, K, KUN, LBG and PE. Photographs of specimens housed at AMES, AU, BR, CAL, CSFI, M and P were also examined. Among the target taxa, G. biflora is by far the most well-represented species in herbaria, and we did not find any additional specimens of the new species. Furthermore, we examined the distribution of multiple characters (e.g. leaf reticulation, flower number and indumentum, color and margin of the labellum) in all species of Goodyera subsect. Reticulum in China, and these observations were summarized in the form of an identification key.

Figure 1. 

Goodyera medogensis and three morphologically similar species in situ A G. medogensis (from Medog, Tibet) B G. hemsleyana (from Malipo, Yunnan) C G. biflora (from Medog, Tibet) D G. vittata (from Yingjiang, Yunnan). Photographs A, C, D by Huai-Zhen Tian B by Chao Hu.

Voucher specimens of G. medogensis were collected in Medog County and preserved at the herbarium of South China Botanical Garden, Chinese Academy of Sciences (IBSC) and East China Normal University (HSNU). Fresh leaves used for molecular analyses were preserved in silica gel. The morphological description of the novelty is based on living material. Measurements were performed with a ruler (0.5 mm accuracy), and small plant parts were observed and measured under a stereo microscope (Mshot-MZ101).

To study the phylogenetic position of the new species within the genus Goodyera, three DNA fragments, viz. internal transcribed spacer (ITS) and two plastid DNA regions (matK and trnL-F), were selected for building the phylogenetic tree based on previous studies (Hu et al. 2016; Zhou et al. 2020). In total, 33 species represented by 80 samples of Goodyera were analyzed with one sample of Zeuxine flava (Wall. ex Lindl.) Benth. ex Hook. f. as the outgroup. All sequences were downloaded from GenBank except those of the new species. Species names and GenBank accession numbers are provided in the supplementary materials (Suppl. material 1: Table S1).

Total genomic DNA of the new species was extracted from silica gel-dried leaves using a modified CTAB method (Doyle and Doyle 1987). Polymerase Chain Reaction (PCR) amplification was carried out on TAKARA TP600 thermocycler (TAKARA BIO INC, Japan) using 25 μl reactions containing 12.5 μl 2× Taq PCR Master Mix (HuaGene, China), 8.5 μl ddH2O, 1.5 μl of each primer (10 μM) and 1 μl target DNA template. Detailed information of primers of relevant DNA fragments used in PCR amplification and sequencing, as well as the procedures of PCR, can be found in Suppl. material 1: Table S2. The resulting amplicons were visualized by horizontal agarose gel electrophoresis (1%), colored with GoldView I (Solarbio, China). Clearly distinguishable bands were recorded, and then the corresponding PCR products were sequenced by Shanghai HuaGene Biotech Co., Ltd (Shanghai, China).

Sequences were firstly assembled and edited with Seqman (DNA STAR package, Madison, WI, F USA) and then adjusted manually. Phylogenetic analysis was conducted using PhyloSuite ver. 1.2.2 (Zhang et al. 2020). Sequences were aligned with MAFFT (Katoh and Standley 2013). Ambiguously aligned fragments were removed using Gblocks (Talavera and Castresana 2007) with all parameters at their default settings. Next, matK and trnL-F were concatenated as well as ITS, matK and trnL-F respectively to two datasets. Thus, three datasets were constructed in total: the cpDNA dataset (matK and trnL-F), the nrDNA dataset (ITS) and the nr+cpDNA dataset (ITS, matK and trnL-F). The three datasets were analyzed by using Bayesian inference (BI) and maximum likelihood (ML) methods respectively. Best-fit evolutionary models for Mrbayes and IQ-TREE were selected under the Bayesian Information Criterion (BIC) using ModelFinder (Kalyaanamoorthy et al. 2017). The best-fit models for the Maximum likelihood (ML) analysis are K80+R2 (ITS) and K3Pu+F+R2 (cpDNA, nr+cpDNA), and for Bayesian inference (BI) they are K2P+G4 (ITS) and GTR+F+G4 (cpDNA, nr+cpDNA).

Based on these models, the Maximum Likelihood (ML) analysis was performed with IQ-TREE (Nguyen et al. 2015) for 10000 ultrafast (Minh et al. 2013) bootstraps, and Bayesian Inference (BI) phylogenies were inferred using MrBayes 3.2.6 (Ronquist et al. 2012). BI analysis consisted of two simultaneous runs and four simultaneous Markov Chain Monte Carlo (MCMC) chains, and ran for 3000000 generations with chain sampling every 1000 generations. The average deviation of split frequencies fell below 0.01, and initial 25% of sampled data were discarded as burn-in. The phylogenetic trees were visualized and modified in FigTree version 1.4.3 (Rambaut 2016).

Results

Our ML and BI phylogenetic trees constructed from the three datasets showed that our four samples of G. medogensis cluster into one separate subclade (Figs 24) which is nested in the clade of Goodyera subsection Reticulum S. W. Chung & C. H. Ou (Hu et al. 2016) consisting of eight other species, viz. G. biflora, G. hachijoensis Yatabe, G. hemsleyana, G. hispida Lindl., G. malipoensis Q. X. Guan & S. P. Chen, G. pusilla Bl., G. vittata and G. yamiana Fukuy.. Thus, the results of phylogenetic analyses support the recognition of G. medogensis as a new species belonging to the subsection Reticulum, and has close relationship with G. biflora, G. hemsleyana and G. vittata (Fig. 1).

Figure 2. 

Phylogenetic tree of Goodyera species inferred by Bayesian and maximum likelihood analyses based on the nrDNA (ITS) dataset. Posterior probability (PP) ≥ 0.50 in BI analysis and bootstrap (BS) % values ≥ 50 in ML analysis are indicated above the branches. Dashes mean the nodes are not supported, i.e. the BS value < 50% in the ML analysis or PP < 0.50 in the BI analysis. The samples of the new species are highlighted in orange, and other species of sect. Reticulum are highlighted in bold. The clade of Goodyera sect. Reticulum is highlighted with the green rectangle.

Figure 3. 

Phylogenetic tree of Goodyera species inferred by Bayesian and maximum likelihood analyses based on the cpDNA (matK + trnL-F) datasets. Posterior probability (PP) ≥ 0.50 in BI analysis and bootstrap (BS) % values ≥ 50 in ML analysis are indicated above the branches respectively. Dashes mean the nodes are not supported, i.e. the BS value < 50% in the ML analysis or PP < 0.50 in the BI analysis. The samples of the new species are highlighted in orange, and other species of sect. Reticulum are highlighted in bold. The clade of Goodyera sect. Reticulum is highlighted with the green rectangle.

Figure 4. 

Phylogenetic tree of Goodyera species inferred by Bayesian and maximum likelihood analyses based on the nr + cpDNA (including ITS, matK and trnL-F) datasets. Posterior probability (PP) ≥ 0.50 in BI analysis and bootstrap (BS) % values ≥ 50 in ML analysis are indicated above the branches respectively. Dashes mean the nodes are not supported, i.e. the BS value < 50% in the ML analysis or PP < 0.50 in the BI analysis. The samples of the new species are highlighted in red, and other species of sect. Reticulum are highlighted in bold. The clade of Goodyera sect. Reticulum is highlighted with the green rectangle.

Taxonomic treatment

Goodyera medogensis H. Z. Tian, Y. H. Tong & B. M. Wang, sp. nov.

Figs 5, 6

Type

China. Tibet Autonomous Region: Medog County, Renqingbeng Temple, under evergreen broad-leaved forest, cultivated at Kunming Botanical Garden, 3 August 2021 (fl.), B. M. Wang TYH-2523 (holotype: IBSC, isotype: HSNU).

Diagnosis

Similar to G. hemsleyana, but distinguished by the greenish thick reticulations on the adaxial surface of leaf blades with diffused margin (vs. white thin reticulations with clear margin), inflorescence with more flowers ((6–)12–15 vs. 4–10), petals obliquely obovate-rhombic (vs. obliquely ovate-oblong), labellum yellow or yellowish (vs. white, with light greenish to pinkish tinge at apex) without lamella on blade (vs. with a low bi-lamellate callus), and floral bracts, sepals as well as ovary with shorter trichomes.

Figure 5. 

Goodyera medogensis A habit B inflorescence C flower, lateral (left) and front (right) view, with the arrow showing close-up of trichomes on abaxial surface of sepals D sepals E petals F column with labellum, anther and ovary G labellum H pollinarium I column with part of the ovary. Photographs by Yi-Hua Tong.

Description

Terrestrial herb, 12–25 cm tall. Rhizome 4–6 cm long, 1.5–3 mm in diam., greenish, rooting at nodes. Roots fleshy, 0.7–7 cm long, yellowish brown, with minute root hairs. Stem erect, terete, 4–9 cm long, 2.7–4 mm in diam., pale green, glabrous, with few sheaths at base formed by withered bases of petioles. Leaves 3–7; petiole 1.1–1.6 cm long, sheathing at base; lamina ovate, 1.9–4.2 × 1.5–2.6 cm, obtuse at base, acute at apex, adaxially green to bluish green with greenish-white reticulations, grid lines thick, margin diffused, transverse ones 5–7, abaxially pale green, 5–7-veined. Inflorescence a terminal raceme, laxly (6–)12–15-flowered, spirally arranged, pubescent; peduncle 4–6.5 cm long, pubescent, with 2–3 sheathing bracts; sheathing bracts 1.5–1.9 × 0.6–0.8 cm, oblong-lanceolate, acute at apex, pale green, clasping, more or less pubescent, especially on the margin, 3–5-veined; rachis 3.5–7 cm long, pubescent. Floral bracts ovate-lanceolate, 1.4–1.7 × 0.5–0.6 cm, acuminate to acute at apex, pale green, longer than ovary, sometimes shortly ciliate at margins, pubescent abaxially, trichomes multicellular, up to 0.8 mm long, glabrous adaxially, 3-veined. Flowers resupinate, opening weakly, 8–10 mm long. Sepals 1-veined, acute at apex, olive greenish, with reddish or brownish tinge when old, with dense clavate trichomes outside, trichomes up to 0.5 mm long; dorsal sepal ovate-lanceolate, 8–9 × 2–3 mm, forming a hood with the petals; lateral sepals ovate-lanceolate, 8–9 × 3.5–4 mm. Petals obliquely obovate-rhombic, 8–8.5 × 3–3.5 mm, acuminate to acute at apex, white, with reddish or brownish tinge at central part, glabrous, 1-veined. Labellum oblong-ovate, 6–7 mm long, yellow or yellowish; hypochile with a sac ca. 1.5–2.5 × 2.5–3.5 mm, with glandular hairs inside; epichile oblong to oblong-lanceolate, entire, 5–6.5 × 2–3 mm, margins slightly undulate, obtuse to subacute at apex. Column cylindrical, ca. 1 mm long; rostellum 3.5–4 mm long, bifid, acuminate at apex. Stigma suborbicular, ca. 1 × 1 mm, entire. Anther yellowish brown, ovate, ca. 2.5 × 1.5 mm. Pollinarium 3–3.5 mm long; pollinia 2, oblong-obclavate, 0.8–1 mm long, dull yellowish white, sectile, bifid; caudicles 1.5–1.7 mm long; viscidium narrowly ovate-oblong, 2–3× ca. 0.5 mm, acute at apex, membranous. Ovary plus pedicel terete, 6–7.5× ca. 2 mm, pale green, twisted, pubescent, trichomes blunt, multicellular, consisting of 5–7 cells. Fruit not seen.

Figure 6. 

Goodyera medogensis A habit B leaf C bract D flower, front view E flower, lateral view F sepals G petals H column with labellum, anther and ovary I labellum J pollinarium K column with part of the ovary. Scale bars: 3 cm (A); 2 cm (B); 5 mm (C–I); 4 mm (K); 2 mm (J). Drawn by Jun Cai.

Etymology

The species epithet refers to the type locality, Medog County.

Vernacular name

墨脱斑叶兰 (Chinese pinyin: mò tuō bān yè lán).

Distribution and habitat

This species is currently known only from Medog County, Tibet, China. It grows under evergreen broad-leaved forests at elevations of 1600–2300 m.

Conservation status

During our three surveys in January, June and August 2021, Goodyera medogensis was found in the forests of Medog Town and Beibeng Township of Medog County, where we counted a minimum of 200 individuals. However, since the population assessment of this species in the whole Medog County has not been made, conservation status of this new species is best classified as ‘Data Deficient’ (DD) (IUCN Standards and Petitions Committee 2019). It occurs within a conservation area, and no threats from logging, tourism or poaching have been recorded until now.

Phenology

Flowering in July-August.

Additional specimens examined

Goodyera medogensis (paratypes): China: Tibet Autonomous Region, Medog County, Bari village, elev. 1750 m, 23 June 2021, cultivated in East China Normal University, 10 August 2021 (fl.), J. Huang & M. Sun 21062310 (HSNU); ibid., Medog village, 19 August 2021, H. Z. Tian et al. 21081914 (HSNU); ibid., Gelin village, 21 August 2021, H. Z. Tian et al. 21082102 (HSNU).

Goodyera hemsleyana: India: Senchal, 2100 m, July 1892, Pantling 215 (lectoype: CAL0000000625 (photo); isolectotypes: CAL0000000624 (photo), K00387611, BR0000006573508 (photo), M0226196 (photo), AMES00090573 (photo), P00333538 (photo)).

Goodyera biflora: Nepal: sin. loc., Wallich, Cat. no. 7379 (holotype: K000364600; isotype: K001127259). India: sin. loc., 1900, J. F. Duthie 24164 (K); Mussorie, July 1901, P. W. Mackinnon 25408 (AMES02091486, photo); Himachal Pradesh, Shimla, 1524 m, 30 June 1886, H. Collett. 325 (K); Himalaya, 1981 m, 1844, M. P. Edgeworth 58 (K). China: Tibet, Gyirong Valley, Tsangpo Valley, 2743 m, 6 August 1935, F. Kingdom-Ward 12159 (K); Tibet, Bome, 2 July 1952, P. C. Tsoong 6699 (PE00339319, PE00339320); Anhui, Jinzhai, 1600 m, 6 August 1986, Plant Resource Team D0062 (PE); Guangdong, Fengkai, 16 June 1974, Yue Seven Four 5295 (IBSC0627265); Guangdong, Xinyi, 15 April 1931, S. P. Ko 51307 (IBSC0627264, IBSC0627266, IBSC0627267, PE00339323, PE00339324); Guangdong, Ruyuan, 27 November 1957, L. Teng 5866 (IBSC0627260); Guizhou, Fanjing Mt., 10 September 1987, China-USA Scientific Research Team s.n. (PE00339322); Guizhou, Rongjiang, 10 July 1974, Anonymous 74-913 (IBSC0627261); Henan, Xin County, 10 August 2013, C. S. Zhu, S. J. Li, X. L. Hou, S. X. Zhu, J. M. Li 130808106 (AU066898, photo); Henan, Shangcheng, 19 June 1984, Plant Resource Research Team D0546 (PE00850731); Hubei, Huanggang, 2 October 2018, X. X. Zhu, L. L. Shi, S. S. Duan, M. J. Hu, Q. Lü ZXX18494 (KUN1444572, photo); Hubei, Shiyan, 8 July 2013, S. L. Li GanQL486 (KUN1458343, photo); Hunan, Chengbu, 22 May 2020, L. Wu, W. J. Liu, C. F. Deng 10403 (CSFI071975, photo); Hunan, Sangzhi, 600 m, 22 June 1991, Q. Lin 714 (IBSC0627263); Hunan, Sangzhi, 11 June 2019, X. Li, C. F. Deng, J. L. Li 190611132 (CSFI072175, photo); Hunan, Cili, 2 October 1984, G. X. Xing & Q. Xia 05571 (PE00339318); Sichuan, Hechuan to Mabian, 1934, S. L. Sun 5590 (PE00339322); Sichuan, s. d., P. C. Tsoong 3981 (PE01849749); Yunnan, Wenshan, 25 September 1958, H. T. Tsai 58-8126 (KUN0022202); Zhejiang, West Tianmu, 2 July 1925, D. X. Zhang 266 (LBG00108146).

Goodyera vittata: India: Sikkim Himalaya, J. D. Hooker 336 (holotype: K000364605); Singalelah Range, 2438 m, July 1896, R. Pantling 410 (AMES02091540, photo). China: Tibet, Zayu, 25 July 1980, 2100 m, Z. C. Ni, Y. Z. Wang, D. Ci et al. 0757B (PE00339514); Yunnan, Yingjiang, 14 August 2012, H. Z. Tian & C. Hu 706 (HSNU).

Key to species of Goodyera subsect. Reticulum in China

1 Inflorescence with more than 20 flowers; dorsal sepal less than 6 mm long 2
Inflorescence with less than 20 flowers; dorsal sepal more than 6 mm long 6
2 Leaves green, without white or pale green venation on adaxial surface G. yamiana
Leaves with white or pale green venation on adaxial surface 3
3 Margins of epichile and petals irregularly denticulate G. pusilla
Margins of labellum and petals entire 4
4 Flowers glabrous G. hachijoensis
Flowers pubescent 5
5 Flower diameter 6–7 mm G. malipoensis
Flower diameter 3–4 mm G. hispida
6 Inflorescence mostly with 2 flowers, sometimes up to 6; dorsal sepal 20–25 mm long G. biflora
Inflorescence mostly with 4–15 flowers; dorsal sepal 3–14 mm long 7
7 Leaves adaxially with a white band along midvein G. vittata
Leaves adaxially with white or greenish reticulate venation 8
8 Leaves adaxially with thin and white reticulations with clear margins; labellum white, with light greenish to pinkish tinge at apex G. hemsleyana
Leaves adaxially with thick and greenish reticulations with diffused margins; labellum yellow or yellowish G. medogensis

Discussion

The new species has the typical features of Goodyera sect. Reticulum, i.e., the leaves have reticulations on adaxial surface, and lateral sepals are not reflexed backwards. According to Hu et al. (2016), sect. Reticulum is one of the four sections of Goodyera, and can be further divided into two subsections, viz., G. subsect. Reticulum S. W. Chung & C. H. Ou and G. subsect. Foliosum S. W. Chung & C. H. Ou. Based on morphology, G. medogensis is probably most closely related to G. hemsleyana. However, considering the conflicting results shown by nrDNA and cpDNA, the position of the new species within subsect. Reticulum remains elusive.

Acknowledgements

We are grateful to Mr. Zhen Liu (Medog Forestry Bureau) and Mr. Xi-Xian Wang (China Railway 12th Bureau Group) for their help during field trips. Mr. Jun Cai is thanked for preparing the beautiful line-drawing. We also thank the curators and staff of the herbaria mentioned above for facilitating the checking of specimens and Dr. Pankaj Kumar (Kadoorie Farm & Botanic Garden) for his valuable comments. This study was funded by the National Natural Science Foundation of China (grant no. 31870180), Biological Resources Program, Chinese Academy of Sciences (KFJ-BRP-017-33) and Biodiversity Survey, Observation and Assessment Program of Ministry of Ecology and Environment of China.

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Supplementary material

Supplementary material 1 

Table S1, S2. Supplementary file of Goodyera medogensis (Orchidaceae), a new species from Tibet, China

Yi-Hua Tong, Mei Sun, Bing-Mou Wang, Huai-Zhen Tian

Data type: phylogenetic

Explanation note: Details of materials for phylogenetic study and primers for sequence amplication and sequencing.

This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited.
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