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 Carex malipoensis (Cyperaceae), a new species from southeast Yunnan, China
expand article infoYuan-Yuan Li§, Ya-Ping Chen, Li-Qiong Jiang|, En-De Liu, Yuan Luo§, Hua Peng
‡ Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China
§ University of Chinese Academy of Sciences, Beijing, China
| Yunnan University, Kunming, China
Open Access

Abstract

Carex malipoensis, a new species from southeast Yunnan, China, is here described and illustrated. It is morphologically similar to C. trichophylla in sect. Euprepes, but differs from it by its longer inflorescences and peduncles, pendulous spikes, hispidulous female glumes, densely hispidulous utricles, and longer nutlets.

Keywords

Carex sect. Euprepes, Carex trichophylla, morphology, taxonomy

Introduction

As one of the largest angiosperm genera, Carex Linnaeus (1753) (Cyperaceae) comprises an extremely high diversity of about 2000 species (Roalson et al. 2021; WCSP 2021). The genus has a Cosmopolitan distribution. It was placed within the tribe Cariceae and divided into four subgenera by Kükenthal (1909): subg. Psyllophora (Degland 1828) Petermann (1849), subg. Vigneastra (Tuckerman 1843) Kükenthal (1899), subg. Vignea (P. Beauv. ex Lestiboudois 1819) Petermann (1849) and subg. Carex. This classification of Kükenthal had been widely adopted by subsequent researchers for a long time. Recent molecular phylogenetic studies revealed that Cariceae is a natural group, and the previously recognized genera of this tribe, i.e., Cymophyllus Mack. ex Britton & A.Br., Kobresia Willdenow, Schoenoxiphium Nees von Esenbeck, and Uncinia Persoon, should be merged into Carex (Yen and Olmstead 2000; Global Carex Group 2015; Starr et al. 2015; Léveillé-Bourret et al. 2018; Martín‐Bravo et al. 2019; Villaverde et al. 2020; Larridon et al. 2021; Roalson et al. 2021). A framework of the combined giant genus was urgently needed to increase our understanding of Carex. In order to solve this problem, Villaverde et al. (2020) conducted a robust phylogeny of Carex based on molecular data (308 nuclear exon matrices, 543 nuclear intron matrices and 66 plastid exon matrices) and six clades were recognized. Accordingly, Villaverde et al. (2020) proposed an updated infrageneric classification of Carex and classified it into six subgenera: subg. Siderosticta M.J. Waterway, subg. Carex, subg. Euthyceras., subg. Psyllophorae, subg. Uncinia (Pers.) Peterm. and subg. Vignea. However, the classification within the subgenera still remained unresolved, so a more systematic and friendly infrageneric classification system was required. A framework infrageneric classification of Carex was proposed recently which divided Carex into 62 formally named Linnean sections and 49 informal groups based on the current phylogenetic knowledge of Carex (Roalson et al. 2021).

A total of 527 Carex were recorded in Flora of China (Dai et al. 2010), the Catalogue of Life China (Chen and Zhang 2018) recorded 593 species, which represents the most complete and update list of the genus in China. The number of species of Carex continues growing in China as more new species have been reported in recent years (Lu and Jin 2018; Zhang et al. 2018; Jin et al. 2020; Lu et al. 2020; Yang and Liu 2020).

During our field investigations between 2016 and 2018, we collected specimens of an unknown species of Carex in Malipo County, southeast Yunnan Province, China. After careful morphological studies, examination of herbarium specimens and relevant literature, we concluded that it can be assigned to Carex sect. Euprepes based on a combination of some morphological characters: cauline leaves well-developed; leaf blades and involucral bracts elliptic to linear-elliptic, with prominent transverse veins; complex branched inflorescence; spikes androgynous; and presence of utriculiform cladoprophylls (Jiménez-Mejíias et al. 2016) at the base of spikes. Molecular phylogenetic studies indicated that C. sect. Euprepes belongs to the core Carex clade (Starr et al. 2015; Villaverde et al. 2020), and the most recent infrageneric classification framework placed it with the Indica Clade together with species traditionally placed in sections Euprepes and Mapaniifoliae (Roalson et al. 2021). Consisting of seven species, sect. Euprepes are restricted to South and Southeast Asia (Nelmes 1955). Only one species of the section, C. zizaniifolia Raymond (1959), was previously reported from China, distributed in southeast Yunnan Province (Dai et al. 2010; Chen and Zhang 2018). However, after our research on this section, we conclude that our new collections are different from all known species and represent a species new to science. We describe and illustrate it here below.

Materials and methods

The new species was compared morphologically with specimens of other taxa of Carex sect. Euprepes from the following public herbaria A, BM, E, GH, HNU, IBSC, K, KUN, HUH, MO, MT, NY, P, PE, and US [acronyms follow Theirs (continuously updated)] as well as our new collections across China (especially with material collected from southeast Yunnan and neighboring area; herbarium specimens kept in KUN). Meanwhile, protologues and other related taxonomic literature were collated and reviewed. The characters’ data come from specimens measurements and the prologue (C. atrivaginata Nelmes and C. tricophylla Nelmes (1955), C. euprepes Nelmes and C. laosensis Nelmes (1939), C. tavoyensis Nelmes (1948), C. zizaniifolia Raymond and C. poilanei Raymond (1959)). The terminology used by Kükenthal (1909) for the morphological description of Carex species was adopted here. The distribution of the new species was compiled from the herbarium specimen records and our own collections, and shown on the distribution map.

Results

A detailed morphological comparison of the potential new species and the seven species of C. sect. Euprepres is summarized in Table 1. Morphologically, the new species is most similar to C. tricophylla but can be distinguished by the characteristics of culms (70–105 cm long, 2–4 mm thick, sides ribbed in the new species vs. culms 45–65 cm long, 1–1.5 mm thick, sides concave in C. tricophylla); leaf sheaths (1.5–7 cm long vs. 1.2–2 cm long); inflorescences (20–45 cm long, peduncles up to 9 cm long vs. 3–15 cm long, more or less exserted), spikes (15–33 mm long, pendulous vs. 7–10 mm long, erect), female glumes (hispidulous vs. glabrous), utricles (densely hispidulous vs. glabrous below, adpressed-hispid above), and nutlets (4–4.5 mm long vs. 2.25–2.5 mm long). The new taxon can be distinguished from all the other seven species in this section by their culms (length, cross-sectional shape, sides and indumentum); leaves (shape, transverse veins), sheath (length, indumentum and the appendage of mouth); inflorescence bigger (20–45 × 3–5 cm in the new taxa vs. 3–25 × 1–5 cm in the other species) or with different shape (oblong in the new taxon vs. oblong, narrowly oblong or triangular-ovate in other species); peduncles longer (up to 9 cm long in the new taxon vs. scarcely or slightly exserted in other species; only C. atrivaginata has relative long peduncles not exceeding 5 cm); and the female glumes, utricles and nutlets (differences in shape, size and indumentum).

Table 1.

Morphological comparisons between Carex malipoensis and species in C. sect. Euprepes.

Characters C. malipoensis C. atrivaginata C. euprepes C. laosensis C. poilanei C. tavoyensis C. tricophylla C. zizaniifolia
Culm
Length 70–105 cm 65 cm 70 cm unknown 70–105 cm unknown 45–65 cm 60–70 cm
Thick 2–4 mm 3 mm 3 mm 2 mm 2–3 mm 2–2.5 mm 1–1.5 mm 2 mm
Transverse angle obtuse acute obtuse obtuse acute acute, obtuse to subacute obtuse obtuse
Sides ribbed concave concave concave concave ribbed, slightly twisted concave concave
Indumentum hispidulous glabrous glabrous glabrous glabrous glabrous hispidulous upward glabrous
Leaf
Number 3–5 7 4–6 1–4 6–8 unknown 6 or more 8
Shape elliptic narrowly linear-lanceolate elliptic narrowly linear-lanceolate narrowly linear-lanceolate Linear-elliptic elliptic, apex attenuated narrowly elliptic or lanceolate
Length 12–28 cm 13–16 cm 17–24 cm 20–30 cm 18–30 cm 25–28 cm 12–18 cm 15–25 cm
Width 2–3 cm 1–1.3 cm 3–4.5 cm 8–15 mm 1–2 cm 2.3–3 cm 1.2–2 cm 2–2.5 cm
Indumentum glabrous, sparsely hispidulous on undersurface midrib glabrous, apex hispidulous scabrid along veins undersurface glabrous glabrous scabrid along veins undersurface scabrid along veins undersurface scabrid along veins
Leaf sheath
Length 1.5–7 cm 1.5–5 cm 1–2 cm 1.5–2 cm 2–5 cm unknown 1.5–2 cm 1.4–2 cm
Indumentum hispidulous glabrous glabrous below, hispidulous above hispidulous glabrous glabrous hispidulous glabrous
Appendage of sheath mouth not developed not developed prominent small small small prominent small
Inflorescence
Shape oblong oblong narrowly oblong narrowly oblong triangular-ovate narrowly oblong narrowly oblong narrowly oblong
Length 20–45 cm 8–10 cm 10–22 cm 3.5–13.5 cm 12–20 cm 15–25 cm 3–15 cm long 5–6 cm
Width 3–5 cm 2–5 cm 3–4 cm 1–2.5 cm 3–6 cm 1–2 cm 1–2 cm 1–1.5 cm
Peduncles exserted exserted scarcely or slightly exserted scarcely exserted exserted scarcely exserted exserted scarcely exserted
Spikes
Length 15–30 (45) mm 10–25 mm 8–10 mm 5 mm 8–18 mm 5–9 mm 7–10 mm 5–6 mm
Male part vs female part much longer much longer equal longer much longer slightly longer longer slightly longer
Utricles
Shape ovate-elliptic ovate-elliptic elliptic broadly ellipsoid or ellipsoid-obovoid ovate or rhomboid-ovate ellipsoid or obovoid-ellipsoid obovoid or ellipsoid-obovoid ovate-elliptic
Length 6–7.5 mm 6–7.5 mm 4–4.5 mm 4–4.5 mm 4–5 mm 4–5 mm 6–6.5 mm ca. 3 mm
Indumentum densely hispidulous densely hispidulous glabrous, margins hispidulous glabrous unknown glabrous below, adpressed-hispid above glabrous below, adpressed-hispid above densely hispidulous
nutlets ca. 4 mm ca. 4 mm 2.25–2.5 mm 2.25–2.8 mm unknown ca. 2.3 mm 2.25–2.5 mm immature

Taxonomy

Carex malipoensis Yuan Y. Li & H. Peng, sp. nov.

Figures 1, 2

Type

China. Yunnan Province, Malipo County, Mengdong Village, Xiangchunping, 22°54'36.77"N, 104°38'54.09"E, alt. 1850 m, 5 December 2016, E.D. Liu et al. LiuED6425 (holotype: KUN! Barcode 1433368; isotype: KUN! Barcode 1347669).

Figure 1. 

Morphology of Carex malipoensis A habitat B plant C rhizome D inflorescence E spike F leaf blade G sheath H the transverse veins I leaf margin J male glumes K female glume L utricles M achene. Scale bars: 2 mm. Photographed by X.X. Zhu (A, D, E LiuED6425, LiuED5912 KUN), Y.P. Chen (B, C, F, I Y.P. Chen & L.Q. Jiang MLP01 KUN), and Y. Y. Li (G, H, J–K Y.P. Chen & L.Q. Jiang MLP01 KUN; L, M LiuED6425 KUN).

Diagnosis

The new species is most similar to C. trichophylla Nelmes (1955), but differs in inflorescences 20–45 cm long (vs. shorter than 15 cm in C. trichophylla), peduncles up to 9 cm long (vs. more or less exserted in C. trichophylla), spikes pendulous (vs. erect in C. trichophylla), female glumes hispidulous (vs. glabrous in C. trichophylla), utricles densely hispidulous (vs. glabrous below, adpressed-hispid above in C. trichophylla), and nutlets 4–4.5 mm long (vs. 2.25–2.5 mm long in C. trichophylla).

Figure 2. 

Line drawing of Carex malipoensis A habit B male glume C female glume D utricles E achene F part of blade, show the transverse veins. Scale bars: 6 cm (A); 2 mm (B–E). Drawn by Yuan Luo from the type specimen.

Description

Rhizomes elongate. Culms tufted, 70–105 cm long, 2–4 mm in diam, obtusely trigonous, hispidulous, basal sheaths dark brown. Leaves 3–5, basal and cauline, loosely arranged; leaf blade elliptic, 12–28 × 2–3 cm, transverse veins prominent, margin hispidulous-villous, base round to cuneate, apex acute, greyish green when dried; sheaths 1.5–7 cm long, hispidulous, mouth hairy, not developed into prominent appendage. Involucral bracts leaf-like, longer than inflorescence, sheathing; panicles 20–45 × 3–5 cm, with 12–16 branches, single or binate; peduncles up to 9 cm long, reduced toward apex, tenuous, glabrous or slightly pubescent; inflorescence axes sharply trigonous, hairy on edges; bractlets glumiform, ca. 5 mm long, apex awned, awns 4–5 mm long; cladoprophylls utriculiform, 3–4 mm long; spikes bisexual, androgynous, 15–30 (–45) mm long; male part of spike much longer than female part; male part densely many flowered, ca. 2.5 mm wide; female part fewer flowered, 3–4.5 mm wide; male glumes oblong-lanceolate, 6–9 × 1.5–2 (–3.2) mm, awned; female glumes oblong-lanceolate, 3.8–4.2 mm long, pale brown, green at middle, apex acute, midrib extending into a scabrid awn. Utricles ovate-elliptic, 6–7.5 mm long, green to brown, veined, densely hispidulous, apex attenuating into a long beak, ca. 3 mm long, orifice oblique. Nutlets ca. 4 × 1.8–2 mm, dark brown, obovate-elliptic, trigonous. Stigmas 3.

Phenology

Flowering from November to December, and fruiting in May.

Distribution

The new species is currently known from Malipo County in southeast Yunnan at the Sino-Vietnamese border (Fig. 3).

Figure 3. 

Distribution map of Carex malipoensis (▲) and C. trichophylla (•).

Habitat

The new species usually grows in evergreen broad-leaved forests at altitudes of 1100–1850 m.

Etymology

The specific epithet refers to Malipo County of Yunnan Province, China, from where the type specimens were collected.

Common name (assigned here)

Ma Li Po Tai Cao (麻栗坡薹草; Chinese name).

Additional specimens examined (paratypes)

China. Yunnan: Maguan County, Ching-kou Loa-chün-shan, 7 December 1947, K.M. Feng 13677 (KUN0368409; KUN1263725); Malipo County, Mengdong Village, 4 December 2016, E.D. Liu et al. LiuED 6336 (KUN1433717), LiuED6403 (KUN1433131); Malipo County, Laojunshan, Bailingyan, 13 May 2017, E.D. Liu et al. LiuED5912 (KUN1340680); Malipo County, Tianbao Town, Bajiaoping Village,1 December 2018, Y.P. Chen & L.Q. Jiang MLP01 (KUN).

Specimens examined of other species

Carex atrivaginata : Vietnam. Chapa: Pételot, 3179 (P00277787, P00277788); E. Poilane, 27084 (MT00072452, MT00072458); C. euprepes: LAOS. Tawieng, Chiengkwang: 2 April, 1932, Kerr, 20927 (BM001172101, K000291207, K000291208, K000291210, NY04059693, P00282617); C. laosensis: Laos. Pak Munung, Wieng chan: 22 April, 1932, Kerr, 21202 (K000291209, K000291210, P00284722); C. poilanei: LAOS. Phong Saly: 6 September, 1941, E. Poilane, 25984 (MT00117677); E. Poilane, 32994 (MT00072475); C. tavoyensis: Myanmar. Padachaung, Tavoy. 3 April, 1921, P.T. Russell, 1935 (K000999214); C. trichophylla: Vietnam. Chapa (Fig. 3): 1 July, 1930, Pételot, 5325 (GH00027549, P00302178, P00302179, P00302180, US00087306); C. zizaniifolia: China. Yunnan, Pingbian: 1934, H. T. Tsai 62809 (A00027543, IBSC0653006, KUN0368701, PE00030290).

Conservation status

The new species is currently known from Maguan and Malipo Counties in Yunnan, China. Only six collections have been recorded since 1947. It may be classified as Endangered (EN) or Vulnerable (VU) according to the IUCN Red List criteria(IUCN 2012). However, collections of Carex are often deficient, and a solid suggestion is needed based on a comprehensive investigation about the new species. Therefore, we suggest to characterize the conservation status of C. malipoensis as Data Deficient (DD) at present.

Discussion

In comparison with other species of C. sect. Euprepes, C. malipoensis is morphologically most similar to C. trichophylla. Both species have obtusely trigonous culms and elliptic and subpetioleate leaf blades, inflorescences big and loose, and utricles longer than 6 mm, all characters that differ from all the remaining species of C. sect. Euprepes. Despite their shared similarities, C. malipoensis can be distinguished from C. trichophylla in the length of inflorescences and peduncles, and indumentum of sheath and utricles. Specifically, C. malipoensis has much longer culms and spikes, and larger leaves, panicles, and achenes compared with that of C. trichophylla. Moreover, the female glumes and utricles are hispidulous in C. malipoensis, but glabrous in C. trichophylla.

Acknowledgements

We would like to thank Dr. Chun-Lei Xiang and two anonymous reviewers for their constructive suggestions that greatly improved this paper. We are grateful to the staff of herbaria IBSC, KUN and PE for their kind assistance with research facilities. We also thank Dr. Xin-Xin Zhu for providing the photographs of the new species. This work was supported by the National Natural Science Foundation of China (Grant No. 31872649).

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