Research Article |
Corresponding author: Yen-Hsueh Tseng ( tseng2005@nchu.edu.tw ) Academic editor: Pieter Pelser
© 2021 Chih-Yi Chang, Hsy-Yu Tzeng, Yen-Hsueh Tseng.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Chang C-Y, Tzeng H-Y, Tseng Y-H (2021) Cirsium taiwanense (Compositae, Cirsium sect. Onotrophe, subsect. Australicirsium), a new species from Taiwan. PhytoKeys 183: 35-53. https://doi.org/10.3897/phytokeys.183.70119
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A new species of Cirsium, C. taiwanense Y.H.Tseng & Chih Y.Chang from central-northern Taiwan is reported in this article. This species is similar to C. hosokawae Kitam. in having a densely cobwebby abaxial leaf surface, but differs in its yellow (vs. vivid purplish red) corolla and the angle between the midrib and the lateral veins of the leaf, which is acute as opposed to nearly at a right angle in C. hosokawae. Cirsium taiwanense has 2n = 32 chromosomes, which is different from the other species in the Taiwanese subsect. Australicirsium Kitam. (2n = 34). An identification key to the Cirsium taxa of Taiwan is presented.
Australicirsium Kitam., central-northern Taiwan, Cirsium, karyotype analysis, pollen morphology, subsect
The genus Cirsium Mill. (Compositae) contains ca. 250 extant species, with its center of diversity in southern Europe and Caucasia (
The island of Taiwan is located about 150 km off the southeast coast of China, between 21°45'N and 25°37'N, and 119°18'E and 122°06'E. Its climate ranges from tropical to subtropical. Taiwan is at the border between the paleotropical floristic kingdom and the Holarctic floristic kingdom (
According to the infrageneric classification of East Asian Cirsium (
Recently, we discovered an unknown Cirsium belonging to subsect. Australicirsium (sect. Onotrophe) growing in the high mountain areas of central-northern Taiwan. This taxon appeared to be similar to C. hosokawae, with abaxial leaf surfaces covered with dense cobwebby hairs and by having nodding mature capitula. However, it can readily be distinguished from the latter by its yellow flowers, and the smaller angle between the midrib and the lateral veins. The aim of the present study was to elucidate the taxonomic status of this taxon using morphological, palynological and cytological approaches. After detailed examinations, we concluded that the taxon represents a new species and it is here described as Cirsium taiwanense.
We compared the two Taiwanese taxa of subsect. Australicirsium with the unknown taxon. Morphological measurements were made using both fresh and dried specimens. For the morphological description, the terminology used by
Herbarium acronyms follow Index Herbariorum (
Pollen grains were collected from fresh materials, and directly mounted on a stub. After air drying for 24 h at room temperature, the samples were sputter-coated with gold at 10–15 mA for 100 s (Quorum SC7620), and observed with a scanning electron microscope (Hitachi S-3400N). The shape, size and exine ornamentation were recorded using the methods of
Voucher material for Cirsium Mill. subsect. Australicirsium Kitam. pollen morphology and karyotype analysis.
Taxa | Location | Altitude | Coordinate (WGS84) | Collecting date | Voucher number | Pollen morphology | Karyotype analysis |
---|---|---|---|---|---|---|---|
C. arisanense | Taiwan. Hualien County, Xiulin Township, Hsiaofengkou | 2,996 m | 24.16245°N, 121.28716°E | 26 June 2015 | C. Y. Chang 602 (TNM) | ● | ● |
Taiwan. Taichung City, Heping District, Mt. Syue trail 8.9 km | 3,399 m | 24.39229°N, 121.24166°E | 3 Sept. 2015 | C. Y. Chang 756 (TCF) | ● | ||
Taiwan. Nantou County, Ren’ai Township, Rueiyan river pipes road 2 km | 2,215 m | 24.11398°N, 121.20746°E | 27 May 2016 | C. Y. Chang 1275 (TCF) | ● | ||
Taiwan. Nantou County, Sinyi Township, Tataka | 2,609 m | 23.47692°N, 120.89841°E | 9 Feb. 2020 | C. Y. Chang 2733 (TCF) | ● | ||
C. hosokawae | Taiwan. Taichung City Heping District, Mt. Syue trail, Kupo to Mt. Syue East Peak | 3,168 m | 24.38882°N, 121.27348°E | 8 Nov. 2015 | C. Y. Chang 870 (TNM) | ● | |
Taiwan. Hualien County, Xiulin Township, Shangyuankuti, Mt. Nanhutashan | 3,586 m | 24.36042°N, 121.43713°E | 5 Sept. 2016 | C. Y. Chang 1432 (TCF) | ● | ||
Taiwan. Ilan County, Datong Township, Mt. Nanhutashan trail, near Mt. Tochiatun | 2,761 m | 24.36801°N, 121.37971°E | 11 Aug. 2018 | C. Y. Chang 2023 (TCF) | ● | ||
Taiwan. Taichung City, Heping District, Mt. Nanhutashan trail, Sungfengling | 2,648 m | 24.36973°N, 121.37167°E | 27 July 2019 | C. Y. Chang 2477 (TCF) | ● | ||
Taiwan. Taichung City, Heping District, Mt. Nanhutashan trail 8.5 km | 2,707 m | 24.36850°N, 121.37350°E | 31 July 2019 | C. Y. Chang 2499 (TCF) | ● | ||
C. taiwanense | Taiwan. Taichung City, Heping District, Mt. Syue trail 0.9 km | 2,398 m | 24.38520°N, 121.29254°E | 3 July 2015 | C. Y. Chang 620 (TCF) | ● | |
Taiwan. Nantou County, Ren’ai Township, Provincial Rd. No. 14A 21.5 km | 2,657 m | 24.11381°N, 121.22401°E | 2 Oct. 2015 | C. Y. Chang 772 (TNM) | ● | ||
Taiwan. Taichung City, Heping District, Mt. Syue trail 0–1 km | 2,353 m | 24.38486°N, 121.29519°E | 24 Apr. 2016 | C. Y. Chang 1230 (TCF) | ● | ||
Taiwan. Taichung City, Heping District, Mt. Tao | 2,648 m | 24.41766°N, 121.30693°E | 22 July 2018 | C. Y. Chang 1926, 2133 (TCF) | ● |
Karyotype analysis was performed using the procedures of
A distribution map was generated using QGIS ver. 3.4 from the package developed by
The values of the quantitative morphological and palynological traits were determined and their means and standard deviations were calculated (Table
Summary of diagnostic characters of Cirsium Mill. subsect. Australicirsium Kitam. in Taiwan.
Characters | C. arisanense | C. hosokawae | C. taiwanense | |
---|---|---|---|---|
Rosette leaves | Size (cm) | 31.32 ± 13.71a × 5.55 ± 1.27a | 19.69 ± 3.79b × 4.42 ± 1.02a | 26.05 ± 6.18ab × 5.55 ± 1.27a |
Shape | narrowly elliptic | narrowly elliptic to oblanceolate | narrowly elliptic to oblanceolate | |
Angle between midrib and lateral vein (°) | 68.78 ± 5.12b | 83.05 ± 9.88a | 63.04 ± 12.00b | |
Cobwebby hairs on abaxial leaf surface | Absent | present | present | |
Cauline leaves | Size (cm) | 12.70 ± 5.84b × 4.10 ± 2.95a | 17.88 ± 0.78a × 5.26 ± 0.74a | 16.17 ± 5.01ab × 4.85 ± 1.56a |
Angle between midrib and lateral vein (°) | 65.31 ± 9.06b | 83.73 ± 9.53a | 62.30 ± 10.71b | |
Cobwebby hairs on abaxial leaf surface | Absent | present | present | |
Capitula | Mature capitula | erect or sometimes nodding | nodding | nodding |
Size (cm) | 3.05 ± 0.22a × 1.42 ± 0.25ab | 2.60 ± 0.66a × 1.18 ± 0.32b | 3.03 ± 0.22a × 1.61 ± 0.17a | |
Phyllaries | Length ratio (inner vs. outer) | 2.05 ± 0.75ab | 1.46 ± 0.33b | 2.91 ± 0.73a |
Length of the reflexed part of the phyllaries (mm) | 4.88 ± 1.23a | 2.33 ± 0.80b | 2.30 ± 0.18b | |
Number | 81.3 ± 11.5b | 86.3 ± 12.0b | 111.7 ± 13.3a | |
Florets | Length (cm) | 2.54 ± 0.15ab | 2.46 ± 0.21b | 2.63 ± 0.21a |
Inflated part of corolla tube length (mm) | 4.39 ± 0.63b | 4.41 ± 0.46b | 5.11 ± 0.70a | |
Corolla color | Yellow | vivid purplish red | yellow | |
Corolla lobes | Revolute | erect | erect | |
Anther length (mm) | 6.02 ± 0.78b | 6.10 ± 0.51b | 6.86 ± 0.80a | |
Number | 102.7 ± 24.1ab | 84.6 ± 19.5b | 129.7 ± 30.1b | |
Achene | Size (mm) | 3.77 ± 0.16a × 1.49 ± 0.06ab | 3.95 ± 0.13a × 1.37 ± 0.05b | 3.97 ± 0.18a × 1.65 ± 0.07a |
Pappus length (cm) | 1.66 ± 0.04a | 1.29 ± 0.04b | 1.60 ± 0.05a | |
Pollen | Pollen size (P/E, μm) | 47.61 ± 0.80a / 46.37 ± 1.00a | 41.40 ± 0.60b / 41.15 ± 0.75b | 48.50 ± 0.80a / 47.00 ± 1.00a |
Pollen spine length (μm) | 4.25 ± 0.18a | 3.91 ± 0.14ab | 3.20 ± 0.18bc | |
Pollen spine base width (μm) | 4.44 ± 0.26a | 5.33 ± 0.19a | 4.74 ± 0.26a | |
Chromosome number | 2n = 34 | 2n = 34 | 2n = 32 | |
Distribution | Endemic to Taiwan; widely distributed in open mountain areas at 1500–3800 m a.s.l. ( |
Endemic to Taiwan; open areas at 1400–3600 m a.s.l. in central-northern Taiwan. | Endemic to Taiwan; open areas at 1400–3400 m a.s.l. in central-northern Taiwan. |
The abaxial leaf surface of the members of subsect. Australicirsium in Taiwan displays two types of indumentum. Both C. hosokawae and C. taiwanense are densely covered with cobwebby hairs, whereas C. arisanense is without cobwebby indumentum. The angle between the midrib and the lateral veins of the leaves of C. hosokawae is often almost 90°, (60–)82–90°, which differs significantly (p ≤ 0.05) from that of C. arisanense, (49–)57–78° and C. taiwanense (44–)52–73° (Fig.
Comparison of the morphological characters of Cirsium Mill. subsect. Australicirsium Kitam. A C. arisanense Kitam B C. hosokawae Kitam C C. taiwanense Y.H.Tseng & Chih Y.Chang 1 cauline leaf, adaxial view 1' cauline leaf, abaxial view 2 capitulum 3 inner phyllary 3' middle phyllary 3" outer phyllary 4 floret 4' floret (pappus removed) 5 synantherous stamens 6 style branches 7 achene 7' achene with pappus.
The pollen grains of Taiwanese species of subsect. Australicirsium are tricolporate, spheroidal and of medium size. The pollen grains of C. taiwanense (43.5–51.8 μm) and C. arisanense (42.9–53.0 μm) have a significantly (p ≤ 0.05) larger diameter than those of C. hosokawae (36–46 μm) (Fig.
The most common chromosome number of Cirsium species is 2n = 34 (
Karyotype of Cirsium Mill. subsect. Australicirsium Kitam A C. arisanense Kitam., 2n = 34 B C. hosokawae Kitam., 2n = 34 C C. taiwanense Y.H.Tseng & Chih Y.Chang, 2n = 32 1 cell 2 karyogram. *relatively clear satellites; arrow indicates chromosomes with secondary constriction. Scale bar: 5 μm.
Although the chromosomes of the three taxa were too short to determine their karyotypic formula, satellites and secondary constrictions could be observed in longer chromosomes. Satellites were observed in C. arisanense for the 3rd, 6th and 7th pairs (Fig.
Cirsium arisanense is the most common Cirsium species in high altitude regions in Taiwan (see distribution map in
Distribution map of Cirsium hosokawae Kitam. (▲) and C. taiwanense Y.H.Tseng & Chih Y.Chang (○) in Taiwan. The red interrupted lines indicate major geographical climate regions as per
Cirsium taiwanense has a unique combination of morphological characteristics: its corolla lobes are erect and yellow, and the abaxial surface of the leaves is densely covered with cobwebby hairs (Fig.
1 | Biennial herb; leaves villose; involucre cylindrical or urceolate | 2 |
2 | Stem without wings; mature capitula nodding; involucre cylindrical | C. ferum |
2* | Stem with spiny wings; mature capitula erect; involucre urceolate |
C. vulgare
|
1* | Perennial herb; leaves glabrous, pubescent or densely cobwebby; involucre urceolate or cupuliform | 3 |
3 | All leaves cauline, basal rosette leaves absent | 4 |
4 | Leaves densely cobwebby on abaxial surface; mature capitula erect, involucre urceolate; apical parts of inner phyllaries inflated, obtuse; outer phyllaries lanceolate, apex acute without a spine; corolla lobes obviously longer than the inflated part of corolla tube | C. lineare |
4* | Leaves glabrous on both surfaces; mature capitula nodding, involucre cupuliform; apical parts of inner phyllaries acute or acuminate; outer phyllaries elliptical with a long spine at the apex; corolla lobes as long as the inflated part of corolla tube | 5 |
5 | Corollas white; leaves pinnatisect or bipinnatisect, lobes > 1.5 cm wide | C. kawakamii |
5* | Corollas purple; leaves mainly pinnatisect, lobes < 1.2 cm wide | C. tatakaense |
3* | Leaves in both a basal rosette and cauline | 6 |
6 | Reflexed part of the phyllaries blade-like; corolla lobes as long as the inflated part of corolla tube | 7 |
7 | Corollas white or light purple | 8 |
8 | Corollas white; inner and outer phyllaries similar in length; stems cauline, without rhizome | C. brevicaule |
8* | Corollas light purple; inner and outer phyllaries distinctly different in length; stems both cauline and rhizomatous | C. morii |
7* | Corollas yellow or vivid purplish red | 9 |
9 | Abaxial leaf surface without cobwebby indumentum; mature capitula erect or nodding; corolla lobes revolute | C. arisanense |
9* | Abaxial leaf surface densely cobwebby; mature capitula nodding; corolla lobes erect | 10 |
10 | Corolla vivid purplish red; angle between midvein and lateral veins of leaf (60–)82–90° | C. hosokawae |
10* | Corolla yellow; angle between midvein and lateral veins of leaf (44–)52–73° | C. taiwanense |
6* | Reflexed part of the phyllaries spine-like; corolla lobes shorter than the inflated part of corolla tube | 11 |
11 | Abaxial leaf surface densely cobwebby; mature capitula nodding | C. suzukii |
11* | Leaf surfaces pubescent, but not cobwebby; mature capitula erect | 12 |
12 | Corolla white; leaves glabrescent | C. japonicum var. takaoense |
12* | Corolla purple; leaves villose, not glabrescent | 13 |
13 | Apical spines of phyllaries shorter than 2 mm; leaves pinnatifid to pinnatisect | C. japonicum var. australe |
13* | Apical spines of phyllaries longer than 3 mm; leaves pinnatipartite to pinnatisect | C. japonicum var. fukienense |
Differs from C. hosokawae in having a yellow corolla (vs. vivid purplish red corolla), a narrower angle between the midrib and lateral veins of the cauline leaves ((44–)52–73° vs. 82–90°), and usually more florets (101–135(–194) vs. 54–111) and phyllaries (90–127 vs. 68–109) per capitulum. Differs from C. arisanense by its nodding mature capitula (vs. erect), erect corolla lobes (vs. revolute), and a densely cobwebby abaxial leaf surface (vs. without cobwebby indumentum).
Taiwan. Nantou County, Ren’ai Township, Provincial Rd. No. 14A 21 km, 2605 m alt., 24.11438°N, 121.21821°E, 15 July 2020. C. Y. Chang 2976 (holotype: TCF; isotype: TNM, PPI).
Perennial herbs, stems 0.5–1.0 m tall, internodes terete. Leaves pinnatipartite or pinnatisect, space between pinnae V-shaped, adaxial surface puberulent or cobwebby, abaxial surface densely cobwebby, margin spinose; rosette leaves narrowly elliptic to oblanceolate, base cuneate to attenuate, apex narrowly acute, 19.5–34.1 × 4.1–7.4 cm, angle between the midrib and the lateral veins (40–)55–76°; pinnae 7–11 pairs, 0.9–2.6 ×1.2–2.1 mm, space between pinnae 0.4–1.2 cm, petiole 1.5–4.0 cm; cauline leaves narrowly elliptic to narrowly triangular, base cordate, apex narrowly acute, 9.0–25.5 × 2.2–6.9 cm, angle between the midrib and the lateral veins (44–)52–73°; pinnae 5–8 pairs, 1.3–2.1 × 0.8–1.9 cm, space between pinnae 0.6–1.5 cm, sessile. Capitula solitary or 2–6 arranged into racemes or panicles, mature capitula nodding. Involucre urceolate, more or less cobwebby, 2.8–3.4 × 1.4–1.9 cm; phyllaries 90–127, in 5–7 series, apex acute, midrib distinct, (0.3–)0.5–1.4 × 1.5–2.1 mm, length ratio of inner and outer phyllaries 2.2–3.6, the reflexed part of the phyllaries 1.5–3.1 mm long. Receptacle flat, densely bristly. Florets 101–135(–194), 2.2–3.1 cm long, with yellow corolla, corolla lobes 5, linear, erect, 3.0–5.1 × 0.4–0.7 mm, corolla tube fistulose with 2 sections, the inflated section of corolla tube 4.1–5.8 mm long; synantherous stamens 5, anthers brown, 5.9–8.3 mm long, base with caudate extensions, filaments 3.0–4.5 mm long with irregular protuberances; stigmas bifid, styles 2.0–2.5 cm long, style arm 2.6–2.7 mm long, ovaries (1.6–)3.2–4.1 mm long. Achenes oblong, base acute, apex truncate, beige, 3.5–4.5 × 1.5–1.8 mm, compressed, 4-angled, ribbed, beak heart-shaped; pappus copious, plumose, bristles in many series, 1.55–1.65 cm long, forming basal ring, easily shed.
Flowering between June and October and fruiting between July and November.
Endemic species of Taiwan. Cirsium taiwanense grows in open areas between Querus forest and Abies forest at 1400–3400 m a.s.l. in central-northern Taiwan. Cirsium taiwanense usually grows at sunny sites. Common companion species are Artemisia morrisonensis Hayata (Compositae), Lilium formosanum Wallace (Liliaceae), Salix fulvopubescens Hayata (Salicaceae) and Rubus pectinellus Maxim. (Rosaceae).
Tai-wan-ji (臺灣薊).
2n = 32 (Fig.
Pollen grains are tricolporate, spheroidal, micro-reticulate and 46.6–51.8 × 43.5–50.7 μm (P/E ratio: 0.9–1.1). The surface is densely covered with spines that are 2.8–3.6 μm long and 3.9–6.0 μm wide at the base. The distance between spines is 8.2–11.8 μm (Fig.
Cirsium taiwanense is common in north-central Taiwan (Fig.
Taiwan. Hsingchu County, Jianshi Township, Mt. Itsashan, 7 Sept. 1993. C. L. Huang 78 (HAST!). Miaoli County, Taian Township, en route from 99 lodge to Mt. Tapachienshan, 11 Aug. 1985. C. I Peng 8492, 8542, 8543, 8544, 8545, 8546 (HAST!); same loc., 2 Nov. 1996. C. M. Wang 2384 (TNM!); Taian, 13 Sept. 1996. C. H. Chen 1870 (TAIE!); 99 Lodge, 2780 m alt., 2 Nov. 1996. C. M. Wang 2384 (HAST!); Mt. Hsishihshan, 27 Oct. 1976. B. P. Yang 81 (TAIF!). Taichung City, Heping District, Mt. Nanhutashan, 21 Sept. 1969. T. Yamazaki 281 (TI!); Yunleng cabin to Mt. Duojiatunshan, 26 June 1994. C. M. Wang 1026 (TNM!); en route from entrance to Yunleng cabin, 27 July 2019. C. Y. Chang 2478 (TCF); Derji, 3 July 1974. C. I Peng 15 (TAI!); en route from entrance to Chika Lodge, 1 June 2003. C. M. Wang 6908 (TNM!); same loc., 19 June 2009. C. M. Wang 13025 (TNM!); same loc., 20 Jun. 2011. C. I Huang 5273 (HAST!); same loc., 16 July 2009. Y. H. Tseng 4697 (TCF); same loc., 9 Feb. 2021. C. Y. Chang 3269 (TCF); en route from Chika lodge to Mt. Syue east peak, 7 Aug. 1986. C. I Peng 9660 (HAST!); same loc., 11 Sept. 2002. C. I Huang 1238 (HAST!; TNM!); same loc., 21 June 2011. C. I Huang 5290 (HAST!); en route from Chika lodge to 369 Lodge, 15 June 1985. C. I Peng 7887 (HAST!); behind 369 Lodge, margin of Abies forest, 9 Sept. 2009. C. T. Chao 922 (TCF!); en route from Wuling lodge to Mt. Tao, 24 Aug. 1988. C. I Peng 12012, 12020, 12100 (HAST!); same loc., 22 July 2018. C. Y. Chang 2133 (TCF!); en route from Wuling lodge to Taoshan waterfall, 24 Aug. 1988. C. I Peng 12097 (HAST!); Huanshan, 6 July 2006. Z. H. Chen 186 (TAIF!). Nantou County, Ren’ai Township, Guandaoxi, 22 Oct. 1932. S. Sasao s. n. (CHIA!); Hsinjenkang, Provincial Rd. No. 14A 21 km, 8 Sept. 1997. S. H. Wu 384 (HAST!); Provincial Rd. No. 14A 22 km, 3 Aug. 2011. T. W. Hsu 17059 (TAIE!); Mt. Hohuanshan near Yuanfeng, 13 July 1985. C. I Peng 8327 (HAST!); Nenggao Cross-ridge Historic Trail, 28 Feb. 2006. M. J. Chung x22805 (TAIF!); same loc., 31 Jan. 2018. C. Y. Chang 1609 (TNM); Provincial Rd. No. 14A 21.5 km, 2 Oct. 2015. C. Y. Chang 772 (TNM); same loc., 27 Oct. 2015. C. Y. Chang 839 (TNM).
We thank Dr. Chien-Ti Chao (趙建棣) for providing useful suggestions and Ms. Pei-Hua Li (李佩樺), Mr. Chia-Hao Liu (留嘉豪), Sheng-Hua Tseng (曾勝華), Chih-Chi Chang (張之齊) and Ms. Pi-Ling Wang (王碧玲) for field assistance. The first author commemorates Mr. Tai-Yuan Chang (張台員) with this species. This manuscript was edited by Dr. Gary Bentley, an editor with Peerwith. This study was supported by the grant of Ministry of Science and Technology no. MOST110-2313-B-005-033-MY3 to Yen-Hsieh Tseng (曾彥學).