Research Article |
Corresponding author: Sandro Bogdanović ( sbogdanovic@agr.hr ) Academic editor: Lorenzo Peruzzi
© 2021 Sandro Bogdanović, Salvatore Brullo, Cristina Salmeri.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Bogdanović S, Brullo S, Salmeri C (2021) Loncomelos koprulense (Asparagaceae), a new species from southern Turkey. PhytoKeys 175: 33-44. https://doi.org/10.3897/phytokeys.175.62037
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A new species, Loncomelos koprulense (Asparagaceae), is described and illustrated from southern Turkey. It is a very rare endemic species growing on small semi-rocky escarpments within the Köprülü Kanyon in the province of Antalya. Morphologically for its hairy leaves, L. koprulense shows some relationships with L. malatyanum and L. tardum, species localized in Anatolia too. The chromosome number of the new species is 2n = 2x = 22. Geographical distribution map for L. koprulense, L. malatyanum and L. tardum is provided.
Distribution, karyology, Mediterranean, Ornithogalum s.l., Ornithogaleae, taxonomy
The genus Ornithogalum L., on account of its remarkable morphological and karyological variability, has been the object of various taxonomical treatments, which led to the recognition of several subgenera, sections and series or its splitting into different genera (
Currently, Loncomelos is represented by ca. 32 taxa, formerly mostly attributed to Ornithogalum, which are characterized by a very variable chromosome complement differing among the species, from diploid to polyploid and even aneuploid assets with 2n = 14, 16, 18, 20, 22, 24, 26, 28, 32, 36, 42, 44, 46, 52, 54, 88 (
The morphological study on the new species was carried out on living material collected from the locus classicus and cultivated in the Botanical Garden of Catania (Italy). Voucher specimens are deposited in the herbarium CAT (abbreviation follows
Main morphological differences among Loncomelos koprulense, L. tardum and L. malatyanum.
Characters | L. koprulense | L. tardum | L. malatyanum |
---|---|---|---|
Plant tall (cm) | up to 95 | up to 80 | up to 73 |
Bulb shape | subglobose | ovoid | ovoid-globose |
Bulb size (cm) | 2.5–3 × 3–3.6 | 2.5–4 × 2.5 | 2.5–2.7 × 1.5–2.5 |
Bulb tunic colour | whitish | grey-brown | whitish |
Scape height (cm) | 55–60 | 40–65 | 34–53 |
Leaf number | 4 | 3–4 | 5–7 |
Leaf length (cm) | 18–30 | up to 35 | (25) 28–40 |
Leaf width (mm) | 3.5–8 | 4–5 | 3–10 (11) |
Inflorescence length (cm) | 32–40 | 23–28 | 12–20 |
Number of flowers | 50–55 | 40 | 18–30 (55) |
Flower pedicel length (mm) | 12–25 | 14–25 | 5–15 |
Bract shape | ovate-lanceolate | subulate | subulate |
Bract length (mm) | 6–13(19) | 10–16 | 7–18 |
Bract margin | smooth | smooth | 0–1 (2) toothed |
Bract / pedicel ratio | shorter to subequal | about a half | equal or longer |
Tepal size (mm) | 10–11 × 2.4–2.6 | 9.5–11.3 × 1.7–2.6 | 9–12 × 2.2–4 |
Tepal shape | linear-oblong | linear | lanceolate to elliptical |
Tepal colour | green, with white margin | greenish, with white margin | whitish, green in the centre |
Tepal margin | undulate | slightly rolled, flat | flat |
Staminal filament shape | oblong, narrowed and apiculate at the apex | oblong, apiculate at the apex | lanceolate, acuminate at the apex |
Staminal filament size (mm) | 5–5.5 × 1.6–2 | 6 × 1.7–1.9 | 5.5–6 × 1.6 |
Anther length (mm) | 2.5–2.7 | 2.8 | 2.2–3.2 |
Anther colour | pale–green | greenish | yellowish-light green |
Ovary shape | ovoid | ovoid | cylindrical |
Ovary size (mm) | 3 × 2.3 | 3–3.5 × 2.2–2.5 | 2–3.3 × 2.2–2.8 |
Style length (mm) | 2.2–2.3 | 3–3.8 | 4–5.3 |
Capsule shape | ovoid | ellipsoid | ovoid to globose |
Capsule size (mm) | 6.5–7 × 5 | 8–9 × 6 | (5) 7–11 × (4) 5–7 |
Chromosome number (2n) | 22 | 20 | 24 |
Leaf anatomy was studied on cross-sections from cultivated plants, using fresh blades of minimum sized and maximum sized leaves in their optimal vegetative phase.
Karyological analyses were performed on root tip cells of cultivated bulbs, pre-treated with a 0.3% (w/v) colchicine water solution for 3 h at room temperature, fixed in Farmer’s fixative (3:1 v/v, absolute ethanol: glacial acetic acid) for 12 h and stored in 70% ethanol water solution. Then, root tips were hydrolysed in 1 N HCl for 7 min at 60 °C and stained according to the Feulgen technique. Microphotographs of at least 10 good metaphase plates from different individuals were taken using a Zeiss PrimoStar microscope equipped with a Canon PowerShot G9 digital camera. Metaphase chromosomes were measured by the Zeiss Axiovision 4.8 image analysis software, while karyotyping was performed by CROMOLAB 1.1 software (
Karyomorphometric parameters and karyotype symmetry indices of Loncomelos koprulense. Values come from 10 good metaphase plates from individuals of the type localities.
Chromosome | TAL (µm) | TRL% | AR | CI | Type | ||||
---|---|---|---|---|---|---|---|---|---|
pairs | Mean ± SD | Max | Min | Mean ± SD | Max | Min | |||
I | 10.8 ± 1.3 | 12.3 | 9.2 | 7.0 ± 0.7 | 7.9 | 6.1 | 1.06 | 48.6 | m |
II | 10.0 ± 1.2 | 11.8 | 8.9 | 6.5 ± 0.3 | 6.6 | 6.4 | 1.13 | 46.9 | m |
III | 8.8 ± 1.0 | 10.1 | 7.4 | 5.7 ± 0.2 | 6.8 | 6.0 | 1.22 | 45.1 | m |
IV | 8.8 ± 1.1 | 10.0 | 7.4 | 5.7 ± 0.4 | 6.4 | 5.2 | 1.49 | 40.2 | msm |
V | 6.9 ± 1.3 | 9.0 | 5.1 | 4.5 ± 0.4 | 5.1 | 3.8 | 2.49 | 28.6 | sm |
VI | 6.5 ± 1.3 | 7.8 | 4.9 | 4.2 ± 0.6 | 5.1 | 3.6 | 1.39 | 41.8 | msm |
VII | 5.9 ± 0.6 | 6.7 | 5.3 | 3.9 ± 0.3 | 4.4 | 3.5 | 2.69 | 27.1 | sm |
VIII | 5.4 ± 0.8 | 6.7 | 4.5 | 3.5 ± 0.1 | 3.8 | 3.3 | 2.17 | 31.5 | sm |
IX | 4.9 ± 0.6 | 5.7 | 4.2 | 3.2 ± 0.1 | 3.4 | 3.1 | 2.38 | 29.6 | sm |
X | 4.5 ± 0.3 | 4.9 | 4.0 | 2.9 ± 0.2 | 3.3 | 2.6 | 1.80 | 35.7 | sm |
XI | 4.5 ± 0.9 | 5.7 | 3.4 | 2.9 ± 0.5 | 3.4 | 2.2 | 1.38 | 42.0 | msm |
TCL: 153.9 ± 17.1 µm; MCL: 7.0 ± 2.2 µm; d-value: 32.5; DRL%: 4.5; S%: 38.3; MAR: 1.54; MCI: 38.7; CvCL: 32.2; CvCI: 21.2; MCA: 24.2; Stebbins’ category: 2B |
Turkey. Antalya: District of Manavgat, Köprülü Kanyon National Park, Bozyaka road, cultivated specimen, 15 June 2010, Brullo s.n. (Holotype: CAT!).
Loncomelo malatyano affine, sed distinctum statura majore, bulbo subgloboso, latiore, 4–foliato, inflorescentia longiore, 50–55 floribus, bracteis ovato-lanceolatis, non dentatis, tepalis lineari-oblongis, viridibus, albis et undulatis margine, filamentis staminorum oblongis, latioribus, ovario ovoideo, stylo breviore, capsula minore.
Plant up to 95 cm tall. Bulbs subglobose, 2.5–3 × 3–3.6 cm, outer tunics whitish, papery, without bulblets. Scape 55–60 cm long, glabrous, green-glaucous. Leaves 4, often withered at the anthesis, shorter than scape, linear, canaliculate, 18–30 × 0.35–0.8 cm, without white median line, abaxial face densely hairy, margins hairy, hairs 0.5–1.2 mm long, adaxial one glabrous. Raceme cylindrical, 32–40 cm long, 50–55 flowered. Pedicels 12–25 mm long, curved-divaricated, glabrous. Bracts membranous, ovate-lanceolate, 6–13(19) mm long, broadened at the base, long acuminate toward the apex, 5–8 nerved, shorter than pedicel or subequal, smooth at the margin, glabrous. Perigon stellate, 20 mm in diameter, tepals linear-oblong, 10–11 × 2.4–2.6 mm, glabrous, papillate-glandulose at the apex, markedly undulate at the margin, green with white margin. Staminal filaments white, oblong, abruptly narrowed and apiculate at the apex, 5–5.5 × 1.6–2 mm, thickened at the margin with central greenish midrib, anthers pale-green, 2.5–2.7 mm, dorsifixed. Ovary ovoid, green, 3 × 2.3 mm, with blunt lobes; each lobe with a distinct central nerve and two smaller, lateral ones. Style 2.2–2.3 mm long, stigma papillose. Capsule ovoid, 6.5–7 × 5 mm, erect. Seeds not seen. Chromosome number 2n = 2x = 22.
Loncomelos koprulense Bogdanović, Brullo & Salmeri, sp. nov. A tepals and stamens B flower with bract C bud with bract D leaf abaxial face E Leaf adaxial face F leaf cross sections. Drawing by S. Brullo from cultivated material coming from the type locality. Darker strips in tepal edges mark the undulations.
Flowering in June and fruiting in June-July.
The species epithet is derived from the name of the Köprülü Kanyon, locality where this geophyte was collected.
All investigated samples of Loncomelos koprulense from the type locality revealed a somatic chromosome complement with 2n = 22 (Fig.
The known Loncomelos species are usually differentiated by their canaliculate leaves, uniformly colored with dorsi-ventral arrangement, presenting differences in size in the same individual. In particular, the leaf outline is smooth in adaxial faces and more or less ribbed in the abaxial one, with epidermal cells covered by a thickened cuticle; the pallisade tissues is one-layered and distributed along the whole perimeter, while the inner part is occupied by the spongy tissue (
Loncomelos koprulense seems to be a very rare species currently know only for a single locality of southern Turkey. One small and well circumscribed population was surveyed along the Bozyaka road within the Köprülü Kanyon at about 150 m of elevation (Fig.
Turkey. Antalya: District of Manavgat, Köprülü Kanyon National Park, Bozyaka road, cultivated specimen, 24 June 2013, Brullo s.n. (paratype: CAT!).
From the literature data (
A very peculiar and significant morphological character occurring in Loncomelos koprulense is the densely hairy leaves (Fig.
Loncomelos malatyanum (Mutlu) Bogdanović, Brullo & Salmeri comb. nov. ≡ Ornithogalum malatyanum Mutlu in Mutlu and Karakuş Turkish Journal of Botany 36: 126 (2012), basionym.