Research Article |
Corresponding author: Vítězslav Plášek ( vitezslav.plasek@osu.cz ) Academic editor: Matt von Konrat
© 2020 Łukasz Krajewski, Lubomír Adamec, Marta Saługa, Halina Bednarek-Ochyra, Vítězslav Plášek.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Krajewski Ł, Adamec L, Saługa M, Bednarek-Ochyra H, Plášek V (2020) Welcome to the Czech Republic again! Rare northern mosses Calliergon megalophyllum and Drepanocladus sordidus (Amblystegiaceae) in South Bohemia in light of their European distribution and habitat preferences. PhytoKeys 154: 111-136. https://doi.org/10.3897/phytokeys.154.51454
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Two aquatic moss species, Calliergon megalophyllum and Drepanocladus sordidus (Amblystegiaceae, Bryophyta), which had been considered extinct in the Czech Republic, were found in the Třeboň Basin, South Bohemia, in 2016–2017. They co-occurred in extensive reed- and sedge-dominated fen pools with humic water on the shore of an old fishpond and the former species was also discovered in a small humic pool in an old shallow sand-pit. The new Czech sites of these rare boreal species represent one of the southernmost known outposts within their entire European range. Previously, the two species were only known from single records in the Czech Republic from the late 19th and early 20th centuries. To confirm our morphological observations, we used phylogenetic analyses of DNA sequence variation in four chloroplast loci (atpB-rbcL, trnL-trnF, rpl16, trnG) and one nuclear region, the internal transcribed spacers of ribosomal DNA (ITS). We found (1) monophyly of all Calliergon megalophyllum specimens tested; (2) based on chloroplast DNA sequences, monophyly among all Drepanocladus sordidus specimens and representatives of Pseudocalliergon turgescens and P. lycopodioides moss species; (3) based on nuclear ITS sequences, monophyly of all original D. sordidus specimens. These results corroborate morphological studies and thus confirm the existence of natural sites for the studied moss species in the Třeboň Basin, South Bohemia, Czech Republic.
Aquatic mosses, Drepanocladus tenuinervis, glacial relicts, Hypnum moldavicum, southern distribution, threatening, Třeboň Basin
During a botanical excursion in August 2016 to the Ptačí blato fishpond in the Třeboň Basin (South Bohemia, Czech Republic), the lead author found two interesting aquatic moss species, Calliergon megalophyllum Mikut. and Drepanocladus sordidus (Müll. Hal.) Hedenäs. They have recently been considered extinct in the Czech Republic (category RE;
Calliergon megalophyllum was recorded for the first time in Central Bohemia and the specimen was described as a new species, Hypnum moldavicum Velen. (
Calliergon megalophyllum is a panholarctic, subarctic-boreal species having a strongly dissected geographical range (Fig.
In the Czech Republic, Calliergon megalophyllum was collected only once in 1896 in Štĕchovice near Prague on the right bank of the Vltava river (German: Moldau) and described as Hypnum moldavicum. It has never been confirmed there since as this site was probably flooded by the dam reservoir and, accordingly, the species has been considered extinct (
Drepanocladus sordidus occurs in slightly mineral-rich to eutrophic habitats. It was recorded growing as a submerged or amphibious species in lakes, backwater pools and oxbows, terrestrial wetland habitats or fens. It is a panholarctic species having a strongly discontinuous geographical range in boreal and temperate zones (Fig.
Although there are many known sites of the two aforementioned moss species, reliable data on biotic and abiotic habitat conditions are still scarce in the literature. In this paper, we characterise water chemistry and vegetation composition at both Czech sites. Also, we compare these characteristics with those at other European sites and discuss the phytogeographic particulars of both species in Europe. As the two species are rare and vanishing in the neighbouring countries (
To investigate whether genetic data support species designation of individuals of Calliergon megalophyllum and Drepanocladus sordidus collected from the Třeboň Basin, Czech Republic, we sequenced the following DNA regions: nuclear ITS (internal transcribed spacers ITS1-5.8S-ITS2 of ribosomal DNA), and selected plastid DNA regions (intergenic spacer atpB-rbcL, intron of the rpl16 gene, intron of the tRNAGly (UCC) gene trnG, and trnL gene plus the adjacent trnL-trnF (GAA) spacer together as a single amplicon). In the first step, our original data was compared to the available nucleotide sequences of reported moss species found in the GenBank database. Secondly, apart from collected individuals, additional herbarium specimens of C. megalophyllum and D. sordidus from Poland and Finland were used as a part of the molecular research. Finally, the taxonomic status of described species was resolved using two methods: maximum likelihood (ML) and Bayesian inference (BI) employed for the dataset consisting of our original sequences supplemented by the GenBank resources. Hence, we placed described species into the larger context of Amblystegiaceae taxonomy of their closely related taxa, e.g. Calliergon (Sull.) Kindb., Loeskypnum H. K. G. Paul, Straminergon Hedenäs, Warnstorfia Loeske in the case of C. megalophyllum, and Cratoneuropsis (Broth.) M. Fleisch., Drepanocladus (Müll. Hal.) G. Roth, Pseudocalliergon (Limpr.) Loeske concerning D. sordidus. Lastly, we checked the morphological identification of these species with their clustering in phylogenetic trees based on nuclear and plastid DNA.
The nomenclature of mosses follows
Calliergon megalophyllum and Drepanocladus sordidus were firstly found in the 1st fen pool (as counted from the north) on the eastern shore of Ptačí blato fishpond, ca. 4 km west of Lomnice nad Lužnicí, Třeboň Basin Biosphere Reserve, South Bohemia, Czech Republic, in August 2016 (for details of the site, see
On 12 October 2017, Calliergon megalophyllum was also found in a small shallow humic pool (area ca. 70 m2, depth 3–50 cm) in an old sand-pit complex near Branná, ca. 4 km SE from Třeboň (
In July 2017, Calliergon megalophyllum was searched for comparison in NE Poland, in three historically known sites in Suwałki Landscape Park (cf.
To specify the occurrence of both rare moss species at potential, humic mesotrophic sites within the Třeboň Basin, an extensive search for both species was conducted at 44 sites in this region in the 2017 and 2018 seasons (see the list in the Suppl. material
Calliergon megalophyllum is a stenotypic species which exhibits a narrow range of morphological variability and is easily distinguishable from other congeners. It is usually a very robust moss, usually growing submerged and often floating, in somewhat nutrient-rich lakes, oxbow lakes and other small water bodies. The plants are green, brownish or yellowish, with shoots 15–30 cm long (exceptionally to almost 1 m in Sweden, L. Hedenäs pers. com.), radially, slightly pinnately or irregularly branched. The stem leaves are erect-spreading, concave, broadly rounded or rounded-ovate to rounded ovate-cordate, 3.5–5.0(–6.0) mm long, 2.5–3.0(–4.5) mm wide, broadly rounded at the apex and abruptly narrowed into a short blunt point. The costa is single, unbranched, 40–80(–105) µm wide at the base, ceasing just below the leaf apex. The alar cells are large, hyaline and thin-walled, forming a large pellucid group sharply separated from the adjacent laminal cells and occupying nearly two third or less the leaf base. Calliergon megalophyllum is closely related to C. richardsonii, but the latter differs in its shorter costa, ending far below the apex which is branched or spurred below and usually forked at the apex. The costa of C. megalophyllum is clearly thinner than that of C. giganteum, in which it is strong or very strong, 90–280 μm near the base, but it is also unbranched and extends to the leaf apex. The alar cells of Calliergon cordifolium (Hedw.) Kindb. form also a large group, reaching almost the costa but it is diffusely delimited and they transform gradually into laminal cells. Finally, a good differentiating character between C. megalophyllum and C. richardsonii is found in the relative length of the distal cell of the axillary hairs (more elongate in C. richardsonii). Since such hairs are abundant and large in Calliergon s.str., this is a useful character, especially when the identity of specimens is doubtful (L. Hedenäs pers. com.).
Drepanocladus sordidus belongs to the D. sendtneri-group species characterised by the presence of rounded-triangular groups of alar cells reaching from the leaf margin one third to two thirds of the distance to the costa. This character immediately distinguishes it from the D. aduncus group in which the alar cells form much larger, triangular groups reaching the costa. Drepanocladus sordidus is closely related to D. sendtneri, but it has a relatively weak costa, 30–75 µm wide near the base which vanishes far below the leaf apex and thin-walled alar cells. In contrast, D. sendtneri has a strong costa, (50-)70–100 µm wide near the base, ending in the leaf acumen near the apex and the alar cells have mostly incrassate walls. According to
To keep living material of the two exceedingly rare moss species available for any study purpose, a simple outdoor culture of them originating from 2–3 specimens collected from Ptačí blato (first pool) has been established in the collection of aquatic and wetland plants in the Institute of Botany CAS at Třeboň (CZ 0 HBT 2017.03851; CZ 0 HBT 2017.03802). The cultivation mimicked the natural conditions of both species in humic water and was the same as that used for growing aquatic temperate Utricularia species (e.g.,
One sample of Calliergon megalophyllum and Drepanocladus sordidus was collected in the field (Třeboň Basin, Czech Republic), with voucher specimens deposited in the Bryophyte Herbarium at the W. Szafer Institute of Botany of the Polish Academy of Sciences (
Gametophyte fragments of weight equal to 12 mg of both herbarium and fresh samples were used for DNA extraction. Total genomic DNA was isolated from six individuals with the Isolate II Plant DNA Kit (Bioline, Meridian Life Science, Memphis, USA) following the manufacturer’s guidelines. We amplified and sequenced five DNA regions that were previously used to analyse the phylogenetic relationships in these genera. Accordingly, the internal transcribed spacers (ITS1-5.8S-ITS2) of nuclear DNA, and the following plastid regions: atpB-rbcL, trnL-trnF, trnG, and rpl16 were used on three individuals of C. megalophyllum. The internal transcribed spacers (ITS1-5.8S-ITS2) of nuclear DNA, and plastid atpB-rbcL, trnL-trnF regions were tested on three individuals of D. sordidus. The analysed DNA fragments were amplified with primers according to PCR conditions described in
To evaluate the genetic designation of described species we combined the sequences newly obtained in this study with the previously published sequences collected from BLAST searches of the GenBank database. In the first step, our original sequences were compared to molecular data of Calliergon megalophyllum (Sweden, MAC B88612), and Drepanocladus sordidus (USA, S B39576) specimens. Afterwards, selected molecular data published by
In the BI analyses, each target plastid region was treated as a separate partition during the analyses. Here, two independent runs starting from random trees were applied, each using four Markov chains. All analyses were run for 10,000,000 generations with sampling trees every 100 generations. In the final analysis 25 per cent, burn-in trees were discarded and the remaining trees and their associated parameter values were saved. The convergence of the chains was determined by examining the plot of all parameters values and the -lnL against generation time using the programme Tracer v.1.5, as recommended by
A list of (micro)sites where the mosses were recently recorded.
Calliergon megalophyllum
Czech Republic:
1a) 3.5 km WNW of Lomnice nad Lužnicí town, Ptačí blato fishpond, NE part, loc. Zátoka 1, 49°5.45'N, 14°40.183'E, and nearby fen meadow, 49°5.4184'N, 14°40.2793'E, 434 m a.s.l., leg. Ł. Krajewski, 20 Aug 2016 (
1b) 3.5 km WNW of Lomnice nad Lužnicí town, Ptačí blato fishpond, NE part and nearby fen meadow, loc. Zátoka 2, 49°5.4106'N, 14°40.2562'E, 434 m a.s.l., leg. R. Ochyra with V. Plášek, H. Bednarek-Ochyra, Ł. Krajewski and L. Adamec, 28 June 2017 (
1c) 3.5 km WNW of Lomnice nad Lužnicí town, Ptačí blato fishpond, NE part and nearby fen meadow, loc. Zátoka 4, 49°5.3873'N, 14°40.231'E, 434 m a.s.l., leg. R. Ochyra with V. Plášek, H. Bednarek-Ochyra, Ł. Krajewski and L. Adamec, 28 June 2017 (
2) 4 km SE of Třeboň town, sand-pit near Branná village, small humic pool in the complex on the margin of a forest, 48°58.428'N, 14°47.813'E, 440 m a.s.l., leg. V. Plášek, 12 Oct 2017 (OSTR B-7253,
Poland:
3) 1 km NE of Błaskowizna, mire E of Boczniel Lake (E of Hańcza Lake), hollow in peat, 54°15.458'N, 22°49.433'E, 230 m a.s.l., leg. Ł. Krajewski, 17 Jul 2017 (
Drepanocladus sordidus
Czech Republic:
1a) 3.5 km WNW of Lomnice nad Lužnicí town, Ptačí blato fishpond, NE part, loc. Zátoka 1, 49°5.45'N, 14°40.183'E, 434 m a.s.l., leg. Ł. Krajewski, 20 Aug 2016 (
1b) 3.5 km WNW of Lomnice nad Lužnicí town, Ptačí blato fishpond, loc. Zátoka 3, 49°5.3993'N, 14°40.183'E, 434 m a.s.l., leg. R. Ochyra with V. Plášek, H. Bednarek-Ochyra, Ł. Krajewski and L. Adamec, 28 June 2017 (
Specimens of Calliergon megalophyllum recorded in Ptačí blato fen pools in 2016 were relatively small (shoots 10–20 cm long, leaves up to 4 mm long and 3.5 mm wide) overgrown with periphyton, dark green-brown, with leaves protruding at right angles and distant, just like C. megalophyllum var. natans Karczm. (
Specimens of Drepanocladus sordidus from Ptačí blato pools in 2016 were strongly overgrown with periphyton, usually dark and dying in the opaque, humic water. The alar cells were typical of the species, the habit turned out (10–20 cm), the leaves strongly bent. Thus, the co-occurred form of D. aduncus (Hedw.) Warnst. growing in the pools is macroscopically distinguishable in much less curved leaves.
In the first (northernmost) pool, both Calliergon megalophyllum and Drepanocladus sordidus were found in a scattered stand on an area of >200 m2. The shallow water in the pool is strongly coloured with humic acids, which is partly caused by the adjacent fen meadow. Yet the water chemistry in the pool is also influenced by the nutrient-rich, hard water from the fishpond body, the penetration of which depends on water level (
Only isolated shoots of Calliergon megalophyllum were found interspersed on the eastern fen margin of the first pool adjacent to an afforested fen meadow within Carici elatae – Calamagrostietum canescentis Jílek, 1958 (variant with Comarum palustre L.). They grew in the vegetation dominated by Calamagrostis canescens (Weber) Roth, Agrostis canina L., Juncus effusus, Peucedanum palustre (L.) Moench, Lycopus europaeus L., Viola palustris L., Hydrocotyle vulgaris L. and Sphagnum spp.
Another site of Calliergon megalophyllum was also found in the Třeboň Basin - in a small humic pool in the sand-pit complex near Branná. The very dense and dominant stand occupies an area of >30 m2, which amounts to ca. half of the surface area of the pool. Although the total water column in the moss stand is 20–40 cm deep, the water is completely filled by plant dominants (Comarum palustre L., Calliergon megalophyllum, Hydrocharis morsus-ranae L.) and the moss grows only near the water surface and an apical part of the shoots often emerges. At this site, the moss also grew scarcely along the adjacent shoreline of a large shallow sand-pit pool in a ca. 20 m long reach. It was evident that these plants were dispersed from the small pool during the events of high-water level or transferred by large animals.
In summary, both moss species grew in relatively soft waters (conductivity 8.8–19.8 mS/m) and within a narrow pH range of 5.94–7.04 in the Třeboň Basin and NE Poland (Table
Summary of vegetation and abiotic factors found at microsites of Calliergon megalophyllum (CM) and Drepanocladus sordidus (DS) at CZ: Ptačí blato and Branná sites, Třeboň Basin Biosphere Reserve, South Bohemia, Czech Republic, in 2017 and PL: Błaskowizna, Suwałki Landscape Park, NE Poland. The pools at Ptačí blato are counted from the north.
No. of the pool | Water depth (cm) | Plant dominants | Rare mosses | Electr. conduct. (mS/m) | pH | Comments on rare moss abundance |
---|---|---|---|---|---|---|
a) CZ: Fen pools at Ptačí blato fishpond | ||||||
1st | 0–5 | Phragmites australis, Carex spp., Typha angustifolia, Juncus effusus, Utricularia australis, Lythrum salicaria | CM, DS | 13.5–19.5 | 6.13–6.42 | both species common |
4th | 0–4 | Carex spp., Sparganium erectum, Utricularia ochroleuca, Agrostis canina | CM | 13.8–15.8 | 6.24–6.36 | CM scarcely |
9th | 0 | Calamagrostis canescens, Juncus effusus, Galium uliginosum, Carex rostrata, Lysimachia vulgaris | DS | – | – | DS very scarcely |
b) CZ: Fen meadow E of 1st bay of Ptačí blato fishpond | ||||||
0–5 | Calamagrostis canescens, Juncus effusus, Hydrocotyle vulgaris, Viola palustris, Sphagnum spp. | CM | 15.8 | 6.36 | CM very rare | |
c) CZ: Humic pool in Branná sand-pit | ||||||
Open stand | 20–40 | Comarum palustre, Hydrocharis morsus-ranae | CM | 11.2 | 6.04–6.08 | dominant |
Shaded stand | 20–30 | Comarum palustre, Carex acuta, Lysimachia vulgaris | CM | 8.8 | 5.94–6.10 | dominant |
d) PL: Peat hollow in mire E of Boczniel Lake | ||||||
70–150 (floating) | Comarum palustre, Myriophyllum verticillatum, Hydrocharis morsus-ranae, Sparganium natans, Stratiotes aloides, Calliergon giganteum, Utricularia spp. | CM | 16.9–19.8 | 6.95–7.04 | CM rare |
Sequence alignments of selected target DNA regions support the recognition of two individuals of aquatic moss species in the study area: Calliergon megalophyllum and Drepanocladus sordidus. Our original sequences obtained from the examined specimens are homogeneous in all DNA regions tested. The only minor detected genetic differences, if present, concern original versus GenBank DNA sequences.
Genetic analysis of Calliergon megalophyllum specimens resulted in the uniform plastid and nuclear data within the testing group (Czech Republic,
Similar results can be found by comparing plastid DNA sequences within the group of Drepanocladus sordidus specimens (Czech Republic,
Species recognition of the specimens of Calliergon megalophyllum and Drepanocladus sordidus collected from the Třeboň Basin was well supported by all BI and ML analyses (see Figs
The plastid and nuclear ITS analyses of Calliergon, Loeskypnum, Straminergon, and Warnstorfia produced very similar tree topologies, and both have resulted in a monophyletic, well-supported clade consisting strictly of C. megalophyllum specimens represented by the following individuals: Czech Republic,
In the nuclear ITS trees our original Drepanocladus sordidus specimens (Czech Republic,
Ptačí blato fishpond (about 40 ha) was built at the end of the 16th century and represents one of the ca. 500 fishponds in this basin (
Presumably, Calliergon megalophyllum and Drepanocladus sordidus previously occurred in the area of the recent Ptačí blato fishpond (before the fishpond was built) in shallow, temporary inundations in Caricion nigrae fens, developed on peats (later mostly exploited and inundated), similar to e.g. recent C. megalophyllum sites in The Netherlands (
Also, the other Czech site of Calliergon megalophyllum, a sand-pit near Branná, is a typical man-made wetland in which sand extraction ceased perhaps in the 1950s. The small humic pool inhabited by the moss species is partly surrounded by a mixed forest so that falling leaves and branches permanently increase the trophic status to recent meso-eutrophy, and render the water strongly humic (
The Třeboň Basin in the Czech Republic is a refuge for many glacial relics both of vascular plants and bryophytes, e.g. Eriophorum gracile (
The historic north-west Czech site of Drepanocladus sordidus is also a fishpond adjacent to a peat bog, where one of the largest national populations of Natura´2000 moss, Hamatocaulis vernicosus (Mitt.) Hedenäs, occurs (
Both rediscovered moss species should be classed with a hazard category CR – critically endangered in the Czech Republic, according to the criteria for C. megalophyllum B2ab (III) D2 and D. sordidus A2c A4a B2ab (III) D2.
In our study, accurate and robust morphological species determination is confirmed by genetic homogeneity detected within all analysed
In this study, the sequence variation, mainly related to Pseudocalliergon turgescens, P. lycopodioides, and Drepanocladus sordidus specimens, showed poor taxonomic structuring. The most likely explanation for this, regarding plastid data, could be an interspecific hybridisation, a hypothesis often reported in bryophytes (
In conclusion, our findings, based on morphological and molecular analyses, show that the bryophyte species Drepanocladus sordidus and Calliergon megalophyllum are present in the Třeboň Basin, Czech Republic, where the latter species form there the southernmost known populations in Europe.
This work was financially supported by EU structural funding Operational Programme Research and Development for Innovation, project No. CZ.1.05/2.1.00/19.0388 and by the Ministry of Education, Youth and Sports of the Czech Republic in the “National Feasibility Program I”, project LO1208 “TEWEP”, and project SGS12/PřF/2020. The work of Marta Saługa and Halina Bednarek-Ochyra has been financed through the statutory fund of the W. Szafer Institute of Botany of the Polish Academy of Sciences. Sincere thanks are due to Prof. Ryszard Ochyra, Kraków, Poland, for his invaluable help with the determination of the mosses, critically reading the manuscript and providing invaluable comments, to Dr. Lars Hedenäs, Stockholm for assistance with generating distribution maps and for information on unpublished records of Drepanocladus sordidus in some European countries and regions, and to Marian Wysocki, Kraków, for help with illustrative material. Special thanks are due to Dr. Brian G. McMillan, Glasgow, Scotland, U.K., for correction of the language. This study was supported (to LA) by the Czech Academy of Sciences as a project No. RVO 67985939. Łukasz Krajewski thanks Prof. Bartosz Płachno, Kraków, Poland, for invitation and subsequently join fruitful trip to the Czech Republic in August 2016.
List of 44 sites in the Třeboň Basin, S Bohemia, Czech Republic
Data type: occurrence
Explanation note: List of 44 sites in the Třeboň Basin, S Bohemia, Czech Republic, where an extensive search for Calliergon megalophyllum and Drepanocladus sordidus was conducted in 2017–2018: The following sites were checked: old sand-pit near Spolí-Domanín, Domanínský fishpond near Domanín, pool in a peatbog V Rájích near Spolí, inlet area of Ruda fishpond near Branná, old sand-pit near Ruda fishpond near Branná, Chodec fishpond near Třeboň, Nový u Smitky fishpond near Stará Hlína, humic forest fishpond near Mláka, peatbog at Starý Vdovec fishpond near Stříbřec, peatbogs at Nový Vdovec fishpond near Stříbřec, peatbog near Příbrazský fishpond near Příbraz, peatbog near Výtopa fishpond (W part) near Lutová, humic pool and peatbog near Staré jezero fishpond near Lutová, sand-pit complex Cep I near Suchdol nad Lužnicí, Smržovský fishpond in Smržov, Dvořiště fishpond near Smržov, Potěšil fishpond near Lužnice, Stehlík fishpond near Lomnice nad Lužnicí, fen pools in the extracted fen Karštejn near Val, inlet area of Černiční fishpond near Lužnice, Loužek fishpond near Lužnice, old shallow sand-pit near Ptačí blato fishpond, inlet area of Záblatský fishpond near Záblatí, extracted fen lake near Ponědrážka, pools in the peatbog at Hliníř fishpond near Ponědrážka, peatbog at Švarcenberk fishpond near Ponědrážka, small fishpond in Dunajovická hora near Dunajovice, old sand-pit in Dunajovická hora near Dunajovice, sand-pit near Kramolín, sand-pit near Hluboká u Borovan, small forest fishpond near Jílovice, Žemlička and Horní Rohožný fishponds near Hluboká u Borovan, small humic forest fishpond near Hluboká u Borovan, old extracted sand-pit near the Dračice river near Františkov, old shallow forest sand-pit Bosna near Rapšach, humic Horní Kočvarů fishpond at Velký Londýn near Františkov, old shallow sand-pit at Velký Londýn near Františkov, fen pool in the Dračice river floodplain near Františkov, Skalice, Medenice and Svobodný fishponds near Františkov and humic fishpond Vydýmač in the complex of the Pele peatbog near Chlum u Třeboně..
A comparison of phytosociological releves from world sites of Calliergon megalophyllum (and Drepanocladus sordidus) from the present and literature data
Data type: occurrence
Explanation note: Explanations: S Bohemia, Czech Republic: 1 – Ptačí blato pond, 1st lagoon, 2 – Ptačí blato, fen margin of the 1st pool, 3 - Ptačí blato, 4th lagoon, LA on 27.8.2017, 4 – Branna sand pit; 5 – NE Poland, Suwałki Landscape Park, hollow peat in mire E of Boczniel lake, (+) – present in this mire close to C. megalophyllum, but not in the identical habitat, ŁK on 16, 17 and 29 July 2017; 6 –Poland (10 localities;
Sample information and GenBank accession numbers
Data type: molecular data