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Research Article
Michaelmoelleria (Gesneriaceae), a new lithophilous dwelling genus and species with zigzag corolla tube from southern Vietnam
expand article infoFang Wen‡§, Zi-Bing Xin‡, Long-Fei Fu‡, Shu Li‡, Lan-Ying Su‡, Stephen Maciejewski|‡, Zhang-Jie Huang‡, Truong Van Do#, Yi-Gang Wei§‡
‡ Guilin Botanical Garden, Chinese Academy of Sciences, Guilin, China
§ South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China
| The Gesneriad Society, Philadelphia, United States of America
¶ Graduate University of Science and Technology, Hanoi, Vietnam
# Vietnam National Museum of Nature, Vietnam Academy of Science & Technology, Hanoi, Vietnam

Corresponding author: Truong Van Do ( dovantruong_bttn@yahoo.com )

Corresponding author: Yi-Gang Wei ( weiyigang@aliyun.com )

Academic editor: Eberhard Fischer
© 2020 Fang Wen, Zi-Bing Xin, Long-Fei Fu, Shu Li, Lan-Ying Su, Stephen Maciejewski, Zhang-Jie Huang, Truong Van Do, Yi-Gang Wei.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation: Wen F, Xin Z-B, Fu L-F, Li S, Su L-Y, Maciejewski S, Huang Z-J, Do TV, Wei Y-G (2020) Michaelmoelleria (Gesneriaceae), a new lithophilous dwelling genus and species with zigzag corolla tube from southern Vietnam. PhytoKeys 146: 89-107. https://doi.org/10.3897/phytokeys.146.49731
Open Access

Abstract

Michaelmoelleria, a new genus from southern Vietnam is described with a single species, M. vietnamensis. The new genus is morphologically most similar to Deinostigma and Tribounia but it differs from the latter two by having four fertile stamens. Nuclear ribosomal internal transcribed spacer (ITS) region and plastid trnL-F intron spacer (trnL-F) DNA sequence data from the new genus and eighty-seven species representing 42 genera within tribe Didymocarpeae are used to resolve its generic placement. The molecular evidence reveals that it is most closely related to Cathayanthe rather than Deinostigma and Tribounia. The chromosome number is counted as 2n = 36 that further clarified its distinction comparing to the related genera within tribe Didymocarpeae. A global conservation assessment is also performed and classifies Michaelmoelleria vietnamensis as Critically Endangered (CR).

Keywords

Cathayanthe, cliff-dwelling, Deinostigma, Didymocarpoideae, flora of Vietnam, IUCN, phylogeny, Tribounia

Introduction

The generic delimitations in Asian Gesneriaceae are often ambiguous due to significant overlap in characters between genera (Burtt 1977), which has led to many revisions including synonymization of small and monotypic genera (Möller et al. 2011a, b; Puglisi et al. 2011; Weber et al. 2011a, b; Möller et al. 2016) and establishment or resurrection of new or previously synonymized genera in recent years (Wei et al. 2010; Weber et al. 2011c; Middleton and Triboun 2012; Middleton and Möller 2012; Middleton et al. 2014, 2015, 2018). The combined phylogenetic-morphological approach was therefore performed as it takes both molecular and morphological evidence into account (Wei et al. 2010).

Vietnam comprises 331,000 km2 situated on the eastern Indochinese Peninsula. The flora of Vietnam contains ca. 12,000 vascular plant species (Averyanov et al. 2003). Despite the high species diversity, it is still under-sampled, with only 43 collections per 100 km2 (Middleton et al. 2019). Due to the status of uneven collection among the countries within Southeast Asia, Thailand, for example, has an estimate of 75 collections per 100 km2 (Middleton et al. 2019) resulting in species richness and endemics of Gesneriaceae being much higher than those in Vietnam. Recently discovered new genera such as Somrania D.J.Middleton (Middleton and Triboun 2012), Tribounia D.J.Middleton (Middleton and Möller 2012), Chayamaritia D.J.Middleton & Mich.Möller (Middleton et al. 2015) and Rachunia D.J.Middleton & C.Puglisi (Middleton et al. 2018) further clarified this status. Besides, the uneven collection also seems to occur within Vietnam. Fu et al. (2019) reported no record of Elatostema from Vietnam’s southernmost provinces and partially ascribed this pattern to unequal sampling effort across the country. Therefore, they proposed a more significant sampling effort in southern Vietnam.

As part of ongoing research into the diversity of Gesneriaceae in Vietnam, a group of botanists from the Vietnam National Museum of Nature (VNMN) and the Gesneriad Conservation Center of China (GCCC) undertook an extensive fieldtrip in southern Vietnam in 2018. A plant belonging to Gesneriaceae was collected in La stream, Tay Giang community, Tay Son district, Binh Dinh province, close to the belt of An Khe city, Gia Lai province, southern Vietnam (Fig. 1). Surprisingly, this unknown species was unable to be placed in any genus based on morphological characters. Superficially, it shares some similarities to Deinostigma and Tribounia, but would be easily distinguished from the former by its distinct characters of the long and zigzag corolla tube and rounded corolla lobes and from the latter by its four stamens and a 2-lobed stigma. To confirm the generic placement of this species, molecular and cytological experiments were also performed. After consulting the relevant literature (Wang et al. 1998; Ho 2000; Li and Wang 2005; Phuong 2005; Wei et al. 2010; Wen et al. 2019) along with the molecular and cytological evidence, we concluded that this new species was assignable to a new genus, Michaelmoelleria gen. nov. This new genus will be an addition to the ongoing project of ‘Flora of Vietnam’ and ‘Flora of Cambodia, Laos and Vietnam’.

Figure 1. 

Photos of the new genus, Michaelmoelleria, the population in natural habitat. M. vietnamensis F.Wen, Z.B.Xin & T.V.Do A habitat along the La stream B habit C plants in flower D frontal view of flower in natural habitat E lateral view of flower in natural habitat. Photos by Fang Wen, arranged by Wen-Hua Xu.

Materials and methods

Ethics statement

The locations where this new species was collected were not in any protected area. No specific permissions were required to enter these areas. Our field studies did not involve any endangered or protected species. Special permits to conduct this research were not required.

Material collection

This new species/genus has been monitored in the field by staff from the VNMN and grown by the authors at the nursery of the VNMN and the GCCC since the plants were collected. We also collected leaf materials of this proposed new species, using silica gel to dry them in the field for DNA extraction.

Morphological observations and specimens examined

A study of the new genus Michaelmoelleria and the only currently known species, M. vietnamensis, from southern Vietnam, was undertaken. All available specimens of Michaelmoelleria are stored in the following herbaria in China and Vietnam: IBK and VNMN (herbarium acronyms according to Index Herbariorum; Thiers 2016, 2019). All morphological characters were studied using a dissecting microscope (SZX16, Olympus, Tokyo, Japan). Characteristics were described using the applicable terminology presented by Wang et al. (1998). The morphological comparison with other species was based on the study of living plants in the field, in cultivation in the VNMN and the GCCC, and herbarium specimens.

Genomic DNA extraction, PCR amplification, and Sequencing

To confirm the placement of this new plant, we performed phylogenetic inference of DNA sequence data obtained from the nuclear ribosomal internal transcribed spacer (ITS) region and the plastid trnL-F intron spacer (trnL-F). Eighty-seven species representing 42 genera as in-group and two species representing one genus as an out-group, including nearly all genera within tribe Didymocarpeae, were sampled. DNA extraction, PCR amplification, and sequencing were performed, following Wei et al. (2013). Sequences obtained from this study and GenBank are listed in the Appendix I.

Phylogenetic analysis

Sequence data were edited and assembled using Lasergene Navigator 7.1 (DNAstar, Madison, Wisconsin, USA). Cleaned sequences were aligned with Geneious R11 (Kearse et al. 2012). Regions of ambiguous alignment and sites with more than 80% missing data were excluded during analyses (Sun et al. 2018). Phylogenetic analyses were conducted using Bayesian inference (BI) and maximum likelihood (ML) methods. ITS and trnL-F datasets were used to construct the ML tree independently to evaluate the congruence between two makers. As there were no hard incongruences (Nishii et al. 2015), we performed the following analysis using a combined dataset. Best-fit DNA substitution models were selected using the Akaike Information Criterion (AIC) in Modeltest v 2.7 (Posada and Crandall 1998). Modeltest determined the best models GTR + G + 1 for the combined dataset. BI analyses were based on a Markov chain algorithm implemented in MRBAYES 3.2.6 (Huelsenbeck and Ronquist 2001). Four chains of the Markov chain Monte Carlo (MCMC) simulation were performed for 4,000,000 generations, each with trees sampled every 100 generations. After discarding the first 25% of the trees, the retained ones were used to calculate the node probability (posterior probability). ML analyses with 1000 bootstrap resampling were conducted using an online version of RAxML-HPC2 v8.2.10 (Stamatakis et al. 2008), available at (http://www.phylo.org/index.php/portal/) (Miller et al. 2010) with the gamma model of rate heterogeneity.

Chromosome preparations

Leaf cuttings yielded new root tips when grown hydroponically for 2–3 weeks. The new root tips were then pretreated with a solution of 0.002 mol·L-1 8-hydroxyquinoline at 13 °C for 4–5 h. After fixation for 24 h by Carnoy solution (3:1 ethanol: acetic acid) at 4 °C, dissociate, stain, and squash methods followed (Jong and Möller 2000, Christie et al. 2012). The chromosome numbers were determined in at least 20 cells from 10 different root tips with well-spread chromosomes in metaphase and captured using a light microscope (Leica DM 2500, camera Leica DFC420).

Results

Molecular phylogenetic studies

The aligned matrix of the combined data (80% missing data were excluded) was 1441 characters, 819 for trnL-F, and 622 for ITS. Of the 778 (54.0%) variable characters, 565 (39.2%) were parsimony-informative including indels. BI analysis of the combined dataset resulted in a consensus tree with a well-resolved backbone but included a large polytomy. The BI tree was largely compatible with the best ML tree (Fig. 2).

Figure 2. 

The Bayesian Inference 50% majority rule consensus tree from the combined dataset of trnL-trnF and ITS. Numbers on the branches indicate the posterior probability (≥0.5) of Bayesian inference analysis and bootstrap values (≥50%) of the maximum likelihood. BOLDFACE indicates the new species/new genus. Created by Long-Fei Fu.

Chromosome characteristics

We illustrated the somatic chromosomes of Michaelmoelleria vietnamensis at metaphase in Fig. 3. It possesses small chromosomes, falling in the range from 1.51 to 4.15 µm, and we identified the number of the somatic chromosomes as 2n = 36 (Fig. 3), with two relatively small satellites. The chromosomes are small, and the position of centromere could not be determined so that it would not be allowed a detailed karyotype analysis.

Figure 3. 

Somatic chromosomes at metaphase of Michaelmoelleria vietnamensis F. Wen, Z.B. Xin & T.V. Do, 2n = 36 A–C are from different cells, solid arrow and, satellites. Photos and arrangements by Lan-Ying Su.

Ecology

Plants of the new taxon were mostly inaccessible, growing on vertical sandstone cliffs and the slope of sandstone hills under tropical evergreen broad-leaved forest. Voucher specimens were made in the usual way (Bridson and Forman 1998) from some accessible plants that could be reached from the base of the cliffs. The conservation assessment was prepared following IUCN (2019).

Taxonomic treatment

Michaelmoelleria F. Wen, Y.G. Wei & T.V. Do, gen. nov.

urn:lsid:ipni.org:names:77209568-1

Diagnosis

Michaelmoelleria resembles monotype genus Cathayanthe, but differs from the latter by leaf blade glabrous (vs. sericeous to pubescent in Cathayanthe, same as order followings); calyx actinomorphic (vs. zygomorphic); fertile stamens 4 (vs. 2), stigma 2, both developed ligulate (vs. 1, subcapitate, divided on 1 side); capsule long linear (vs. fleshly, narrowly ellipsoid). The new genus is also morphologically similar to Deinostigma and Tribounia, but is easily distinguished from both by having corolla tube narrowly curving to zigzag infundibuliform-tubular, and bent at about 90° angle in the middle of corolla tube (vs. infundibuliform in Deinostigma; of a narrow lower tube which widens into an infundibuliform & upper tube which has a prominent boss on the dorsal surface in Tribounia, same as order followings), fertile stamens number 4 (vs. 2; 2) and stigma 2-lobed, lobes often gathering together (vs. upper lip usually vestigial and only lower lip developing, broad, flat and weakly 2-lobed; capitate).

Type and only known species

Michaelmoelleria vietnamensis F. Wen, Z.B. Xin & T.V. Do, sp. nov.

Description

Herbs, perennial, epipetric, obvious flesh stem, rosette when young and elongated when aging. Leaves basal or clustered at the top of the stem when young but alternate on elongated aerial stem after years of growth; leaf blade ovate to elliptic, glabrous, base cordate to broadly cuneate, apex obtuse. Inflorescences lax, axillary, 1- or 2-flowered cymes; bracts 2. Calyx actinomorphic, 5-parted to the base. Corolla bluish purple to purple, zygomorphic, inside glabrous; tube obviously curved at the middle, dramatically enlarged to be trumpet-shaped from the middle of corolla tube toward limb, much longer than limb; limb 2-lipped; adaxial lip 2-lobed and abaxial lip 3-lobed, lobes rounded to oblate, apex rounded. Stamens 4, included; anthers basifixed, coherent in pairs, thecae divaricate, confluent at apex, dehiscing longitudinally; staminode 1. Disc annular. Ovary narrowly ellipsoid, 1-loculed; placentas 2, parietal, projecting inward and divaricate. Stigma 2, both developed and appressed, lobes ligulate. Capsule straight in relation to pedicel, linear, dehiscing loculicidally to base, splitting along one suture, straight, not twisted.

Etymology

Michaelmoelleria was named in honor of Prof./Dr. Michael Möller from the Royal Botanic Garden Edinburgh. He is a well-known botanist studying Old World Gesneriaceae, especially in Africa (Madagascar) and Asia (China), and mentor of the senior author from the 1990s to the present. “Michaelmoeller-” (means “Michael Möller”) stands for his full name. “moeller” is the English modification of the German family name, “Möller”. Initially, we planned to use “Moelleria” as the genus name. However, this name was used in different places three times. They are Moelleria Cleve (Bacillariophyta, incertae sedis) [non Moelleria Scop. (Spermatophyta, Flacourtiaceae) (≡ Iroucana Aubl.)]; [nec Moelleria Bres. (Fungi, Clavicipitaceae) (≡ Moelleriella Bres.)] [nec Moelleria (Freng.) Freng. (Bacillariophyta, Naviculaceae)] (Blanco and Wetzel 2016). Thus, to prevent confusion with those mentioned above, three existing and existed “Moelleria”, we consider that using the variant of Dr. Michael Möller’s full name, “Michaelmoeller”, to name this new genus to be most appropriate.

Distribution and habitat

Endemic to southern Vietnam, under broadleaved forests in a montane granite area at 140–200 m altitude.

Michaelmoelleria vietnamensis F. Wen, Z.B. Xin & T.V. Do, sp. nov.

urn:lsid:ipni.org:names:77209569-1
Figs 1, 4

Type

Vietnam. Binh Dinh province, Tay Son district, Tay Giang community, La stream. 13°55'59"N, 108°45'43"E, ca. 148 m, WYG180329-01 (holotype: VNMN!, isotypes IBK!).

Figure 4. 

Photos of new genus, Michaelmoelleria F. Wen, Y.G. Wei & T.V. Do. M. vietnamensis F. Wen, Z.B. Xin & T.V. Do A flowering potted plant in GCCC’s greenhouse B frontal view of the corolla and the corolla tube C different angles of a lateral view of corolla and corolla tube (I) D different angles of lateral view of the corolla and the corolla tube (II) E cyme F bracts (above: adaxial surface; below: abaxial surface) G calyx lobes H lateral view of flower and bud I calyx lobes (the left three: adaxial calyx lobes surfaces; the right two: abaxial calyx lobes surfaces) J opened corolla showing stamens and staminode K our fertile anthers L pistil and calyx M stigma. Photos by Fang Wen, arranged by Wen-Hua Xu.

Description

Herbs perennial, rosette when young and stem obviously elongated after years of growth. Stem fleshy, cylindrical, 6–30 cm long, 4–6 mm in diameter, densely white pubescent when young, but glabrescent to glabrous when aging. Leaves alternate on elongated aerial stem, 12–20 cm or more, nearly clustered near the top of the stem and look opposite. Petiole 4–8.5 cm long, 3–3.5 mm in diameter, densely white pubescent. Leaf-blade slightly fleshly to thickly chartaceous, when dried flimsily chartaceous, ovate to elliptic, glabrous, green to dark green, usually with irregular silvery or argenteous spots on the adaxial surface, but silvery-brown to slight yellowish-brown in dry season, 4.5–7 × 2.5–4 cm, base marginally oblique, often slightly cordate, cordate to broadly cuneate, apex obtuse, margin entire, sinuate or with inconspicuously undulate teeth, adaxially and abaxially erectly puberulent; venation alternate along main vein, lateral veins 5–7 on each side of midrib. Cymes axillary near stem apex, fasciculate, 6–10 flowered per plant; peduncle slender, 8–15 cm long, 1–1.5 mm in diameter, brownish-green, densely erectly puberulent; bracts 2, ovate, both usually deflected to same side, 9.8–10.5× 2.5–2.7 mm, adaxially sparsely puberulent, abaxially sparsely puberulent; 1-flowered and 2-flowered per cyme but one of both often abortive; pedicel 1.5–3 cm long, 1–1.2 mm in diameter, green to lime, sparsely extremely white puberulent. Calyx actinomorphic, 5-parted to the base, segments lanceolate to narrowly lanceolate, 8–8.5 mm long, 2–2.3 cm in diameter at the base, apex acute but top usually formed hammer-shape, margin entire, outside sparsely white puberulent, inside glabrous. Corolla obviously curving to zigzag funnelform, zygomorphic, 8–8.5 cm long, outside bluish-purple to purple, densely glandular and glandular-puberulent, inside purple, nearly glabrous, the color of the throat same as the corolla with two brownish-yellow stripes and sparse dark yellow glands on the surfaces of the two stripes. Corolla tube narrowly curving or zigzag infundibuliform-tubular, bent at about 90° angle in the middle of corolla tube, and gradually slightly swollen from the middle to the base of the tube, 3.5–3.9 mm in diameter at middle/corner and 4.8–5.4 mm at the base of tube; dramatically enlarged to be trumpet-shaped from the middle of corolla tube toward limb, 1.9–2.3 cm wide at the orifice of the corolla limb. Corolla limb 2-lipped, adaxial lip 2-lobed, lobes semi-rounded to slightly obliquely oblong-rounded, 1.3–1.5 × 1–1.2 cm; abaxial lip 1.5–1.9 cm long, 3-lobed, middle lobe rounded to oblate and narrowed at the base of middle lobe, 1–1.1 × 0.9–1 cm, lateral lobes orbicular to slightly obliquely oblong-rounded to oblate, 0.9–0.95 × 1.1–1.2 cm. Stamens 4, bigger pair adnate to corolla tube ca. 2.8 cm from the base and smaller pair adnate to corolla tube ca. 2.5 cm from the base, coherent; anthers glabrous; filaments glabrous to very sparsely glandular-puberulent, but near the top of filaments and the part close to anther densely glandular-puberulent, longer pair 8–9 mm long and shorter pair 7–7.5 mm; anthers glabrous, 2.2–2.5 mm long, margin of locule dark purple to purplish-brown; pollen gray; staminode 1, punctate, adnate to corolla tube 2–2.1 cm from base, ca. 1 mm long. Disc annular, ca. 1 mm high, margin entire. Pistil 8–8.5 cm long; ovary cylindric-linear, glabrous, 3.5–4 cm long, pale green; style linear, densely erect glandular and glandular-puberulent, ca. 4.5 cm long; stigmas 2-lobed, often gathering together but slightly opened at the end of flower, lobes ligulate, pink, sparsely glandular-puberulent at the base of stigma lobes but glabrous from the middle to the top of stigma lobes, 3.6–3.7 mm long. Capsule straight in relation to pedicel, linear, glabrous, 7.5–10 cm long, 2–2.5 mm in diameter, straight, dehiscing loculicidally to base, splitting along one suture, straight, not twisted.

Phenology

Flowering occurs from March to April and fruiting from March to June.

Etymology

The genus is named for the famous botanist, Dr. / Prof. Michael Möller, from the Royal Botanic Garden Edinburgh, and the species is named for Vietnam, which holds the first discovered and only known location for the species.

Preliminary conservation status

Based on the result of our joint field surveys in the type locality and adjacent regions, the EOO and AOO of Michaelmoelleria vietnamensis are about 2.65 km2 and 0.02 km2, respectively. So far, only one population of this species has been recorded along the local stream on the sandstone hills of Binh Dinh province, southern Vietnam, but we believe that more M. vietnamensis populations will be found in the hills and mountains of southern Vietnam. If that is the case, the Extent of Occurrence (EOO) and Area of Occupancy (AOO) of this species will increase. The beautiful flowers and variegated leaves have led to its over-harvesting by local people who have sold it as an ornamental plant. For example, some local people are selling them on the digital networking platform, Facebook. This activity has caused a decline in the quantity of M. vietnamensis. Moreover, our field surveys showed that M. vietnamensis only occurred in the sandstone hills of Tay Giang community, Tay Son district, Binh Dinh province. Man-made Eucalyptus forests have severely fragmented the natural habitat. According to the Guidelines for Using the IUCN Red List Categories and Criteria (IUCN 2019), we access this taxon as a Critically Endangered species (CR B1+B2ab (iii, v)).

Discussion

In March 2018, a plant having bluish-purple flowers of Gesneriaceae was collected in southern Vietnam. Neither the collectors nor the researchers on the family were able to allocate it to any known genus at that time. Collectors once thought that it might be a member of Deinostigma because this genus is distributed from South China to Central Vietnam (Möller et al. 2016), and shows superficial similarities to Deinostigma in general appearance. For example, the caulescent habit (D. cicatricosa, D. cyrtocarpa, D. minutihamata (D.Wood) D.J.Middleton & H.J.Atkins), usually more and less fleshy leaves (in most Deinostigma species except three species as mentioned above) and fleshy stems, but differs in long and zigzag narrowly infundibuliform corolla tube, four fertile stamens and two lingulate stigmas. The other morphological similar genus is Tribounia, a genus endemic to Thailand (Middleton and Möller 2012). The two genera share the characters of zigzag corolla tube and rounded corolla lobes but can be distinguished by the number of fertile stamens. Based on those morphological characters above, we confirmed that it does not belong to Deinostigma or Tribounia. Furthermore, after consulting the related literature (Burtt 1954, 1963; Wang et al. 1990, 1998; Li and Wang 2005; Weber et al. 2011c, 2013), we also could not find any genus in which to place this unknown species.

The phylogenetic relationship was largely congruent with previous studies (Möller et al. 2009, 2011a, 2016; Middleton et al. 2015, 2018). Michaelmoelleria vietnamensis and its morphologically similar genus Deinostigma is recovered within a polytomy but the phylogenetic relationship of them is distant. It also shows a more distant relationship between Michaelmoelleria vietnamensis and the other similar genus Tribounia. However, our analyses of DNA sequence data suggest that Michaelmoelleria vietnamensis is closely related to the genus Cathayanthe with strong support (PP = 1, BS = 100) and both of them sister to the clade comprised of the genera of Allocheilos, Gyrocheilos, Liebigia, and Didymocarpus with strong support of BI analysis (PP = 0.99) but weak support of ML analysis (BS = 69). Morphologically, Michaelmoelleria vietnamensis, which represents this new genus endemic to Vietnam, can be easily distinguished from the genera of Cathayanthe, Allocheilos, Gyrocheilos, Liebigia, and Didymocarpus by these distinct characters: fleshly stem and leaves alternate on elongated aerial stem. In addition, only two genera’s chromosome numbers among above-mentioned genera were reported (Liebigia speciosa (Blume) DC. in Liebigia: 2n = 28 or 32; Didymocarpus ssp.: 2n = 20, 22, 24, 28, 32, 36, 44, 54) (Möller and Pullan 2015 onwards; Yang et al. 2019). Furthermore, the chromosome numbers of Allocheilos W.T. Wang (two species, both endemic to China) and Gyrocheilos W.T. Wang (six species and two varieties, distributed from South China to North Vietnam), which are the relative genera of Michaelmoelleria, had never been reported before (Li and Wang 2005; Middleton 2015). The cytological evidence showed 2n = 36, which is the difference from above genera, except Didymocarpus pedicellatus R.Br. (Mehra and Vasudevan 1972; Vasudevan 1976). Building on these, we treat Michaelmoelleria vietnamensis as a distinct genus of tribe Didymocarpeae. All distinguishing characters for identification of Michaelmoelleria, Cathayanthe (Fig. 5), Deinostigma (Fig. 6A–E) and Tribounia (Fig. 6F–I) are listed in Table 1.

Figure 5. 

Photos of Cathayanthe biflora Chun, the related genus/species of Michaelmoelleria vietnamensis F. Wen, Z.B. Xin & T.V. Do A habitat B cyme and frontal view of flower C cyme and lateral view of flower D fruit. Photos by Fang Wen, arranged by Wen-Hua Xu.

Figure 6. 

Photos of Deinostigma W.T.Wang & Z.Y.Li (A–E) and Tribounia D.J.Middleton (F–I), the morphologically similar genera and species of Michaelmoelleria F.Wen, Y.G.Wei & T.V.Do (M. vietnamensis F.Wen, Z.B.Xin & T.V.Do) A Deinostigma eberhardtii (Pellegr.) D.J.Middleton & H.J.Atkins B D. tamiana (B.L.Burtt) D.J.Middleton & H.J.Atkins C D. cycnostyla (B.L.Burtt) D.J.Middleton & H.J.Atkins D D. cicatricosa (W.T.Wang) D.J.Middleton & Mich.Möller E D. cyrtocarpa (D.Fang & L.Zeng) Mich.Möller & H.J.Atkins F–G Tribounia venosa (Barnett) D.J.Middleton H–I T. grandiflora D.J.Middleton. A–E photos by Fang Wen F–I photos by David Middleton, arranged by Wen-Hua Xu.

Table 1.
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Comparison of morphological characters Michaelmoelleria, Cathayanthe, Deinostigma and Tribounia.

Characters Michaelmoelleria Cathayanthe Deinostigma Tribounia
Life form perennial perennial perennial probably annual
Stem glabrescent to glabrous when ageing rhizomatous, stemless multifarious, from short and constrictive to procumbent; from glabrous to pubescent and puberulent with hairs and often hooked at the tip
Leaves alternate on elongate aeriald stem, 12–20 or more few, 4–8, basal alternate, numerous opposite, numerous
Lea-blade ovate to elliptic, both surfaces glabrous oblanceolate to obovate or elliptic, adaxially whitish to brownish sericeous, abaxially appressed pubescent multifarious, slightly peltate or not, glabrous or eglandular pubescent on both surfaces ovate, densely pubescent adaxially and abaxially mostly with eglandular pubescents (occasional glandular pubescent present in Tribounia grandiflora)
Calyx Actinomorphic, 5-parted to the base zygomorphic, 2-lipped divided to base, elliptic, those on ventral side slightly longer and wider equally 5-partite almost to base
Corolla tube narrowly curving to zigzag infundibuliform-tubular, and bent at about 90° angle in the middle of corolla tube, and gradually slightly swollen from the middle to the base of the tube; dramatically enlarged to be trumpet-shaped from the middle of the corolla tube toward the limb tube tubular, nearly straight to slightly bent, slightly gibbous abaxially toward limb, much longer than limb tube infundibuliform, lower lip 3-lobed, upper lip 2-lobed, lobe apices rounded zygomorphic, of a narrow lower tube which widens into an infundibuliform upper tube which has a prominent boss on the dorsal surfacew
Fertile stamens number 4 2 2 2
Ovary cylindric-linear, glabrous, 3.5–4 cm long narrowly ellipsoid, ca. 6 mm long fusform, long or short, with different indumentum, from glabrous to glandular- and eglandular-puberulent cylindrical, densely glandular pubescent, ca. 6 mm long in T. venosa, 7.5–11.0 mm long in T. grandiflora
Stigma 2-lobed, often gathering together subcapitate, lower part developing ligulate, upper lip usually vestigial and only lower lip developing, broad, flat and weakly 2-lobed capitate

Acknowledgments

Thanks to: Mr. Wen-Hua Xu for his attractive arrangement of photos; Mr. Wuf and Ms. Banbino and VietNam’s Garden (the owner of the Facebook account) for their helpful information about this species and Dr. David Middleton from Singapore Botanic Gardens for his constructive suggestions and photos. This study was financially supported by Vietnam National Foundation for Science and Technology Development (NAFOSTED) under grant number 106.03-2019.308, the Fund of Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain (19-050-6), the National Natural Science Foundation of China (31860047), the Natural Science Foundation of Guangxi (2017GXNSFAA198006), Science Research Foundation of Guangxi Academy of Sciences (2017YJJ23022), Guilin Science and Technology Foundation (20180107-6), the Key Sci. & Tech. Research and Development Project of Guangxi (Guike AB16380053). We also wish to thank Michael LoFurno (Adjunct Professor, Temple University) from Philadelphia, USA for his editorial assistance.

References

  • Averyanov LV, Loc PK, Hiep NT, Harder DK (2003) Phytogeographic review of Vietnam and adjacent areas of Eastern Indochina. Komarovia 3: 1–83.
  • Bridson D, Forman L (1998) The Herbarium Handbook Royal Botanic Gardens (3rd edn.). Kew: Royal Botanic Gardens, London.
  • Burtt BL (1954) Studies in Gesneriaceae of the Old World. 1: General introduction. Notes from the Royal Botanic Garden Edinburgh 21: 185–192.
  • Burtt BL (1963) Studies in Gesneriaceae of the Old World. XXIV: Tentative keys to the tribes and genera. Notes from the Royal Botanic Garden Edinburgh 24: 205–220.
  • Burtt BL (1977) Classification above the genus, as exemplified by Gesneriaceae, with parallels from other groups. Plant Systematics and Evolution 1(Suppl.): 97–109.
  • Christie F, Barber S, Möller M (2012) New chromosome counts in Old World Gesneriaceae: Numbers for species hitherto regarded as Chirita and their systematic and evolutionary significance. Edinburgh Journal of Botany 69(2): 323–345. https://doi.org/10.1017/S0960428612000169
  • Fu LF, Monro A, Do TV, Nuraliev MS, Averyanov LV, Wen F, Xin ZB, Maisak TV, Kuznetsov AN, Kuznetsova SP, Nguyen KS, Wei YG (2019) Checklist to the Elatostema (Urticaceae) of Vietnam including 19 new records, ten new combinations, two new names and four new synonyms. PeerJ 7: e6188. https://doi.org/10.7717/peerj.6188
  • Ho PH (2000) An Illustrated Flora of Vietnam. Vol. 3. Tre Ho Chi Minh City Press, Ho Chi Minh, Vietnam. [in Vietnamese]
  • Jong K, Möller M (2000) New chromosome counts in Streptocarpus (Gesneriaceae) from Madagascar and the Comoro Islands and their taxonomic significance. Plant Systematics and Evolution 224: 173–182. https://doi.org/10.1007/BF00986341
  • Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M, Sturrock S, Buxton S, Cooper A, Markowitz S, Duran C, Thierer T, Ashton B, Meintjes P, Drummond A (2012) Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics (Oxford, England) 28(12): 1647–1649. https://doi.org/10.1093/bioinformatics/bts199
  • Li ZY, Wang YZ (2005) Plants of Gesneriaceae in China. Henan Science and Technology Publishing House, Zhengzhou.
  • Middleton DJ, Triboun P (2012) Somrania, a new genus of Gesneriaceae from Thailand. Thai Forest Bulletin (Botany) 40: 9–13.
  • Middleton DJ, Atknis H, Truong LH, Nishii K, Möller M (2014) Billolivia, a new genus of Gesneriaceae from Vietnam with five new species. Phytotaxa 161(4): 241–269. https://doi.org/10.11646/phytotaxa.161.4.1
  • Middleton DJ, Nishii K, Puglisi C, Forrest LL, Möller M (2015) Chayamaritia (Gesneriaceae: Didymocarpoideae), a new genus from Southeast Asia. Plant Systematics and Evolution 301(7): 1947–1966. http://doi.10.1007/s00606-015-1213-2
  • Middleton DJ, Khew GS, Poopath M, Puglisi C, Möller M (2018) Rachunia cymbiformis, a new genus and species of Gesneriaceae from Thailand. Nordic Journal of Botany 36(11): e01992. http://doi.10.1111/njb.01992
  • Middleton DJ, Armstrong K, Baba Y, Balslev H, Chayamarit K, Chung RCK, Conn BJ, Fernando ES, Fujikawa K, Kiew R, Luu HT, Aung MM, Newman MF, Tagane S, Tanaka N, Thomas DC, Tran TB, Utteridge TMA, van Welzen PC, Widyatmoko D, Yahara T, Wong KM (2019) Progress on Southeast Asia’s Flora projects. Gardens’ Bulletin Singapore 71(2): 267–319. http://doi.10.26492/gbs71(2).2019-02
  • Miller MA, Pfeiffer W, Schwartz T (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Proceedings of the Gateway Computing Environments Workshop (GCE), 2010. Institute of Electrical and Electronics Engineers, New Orleans, 1–8.
  • Möller M, Pullan M (2015 onwards): RBGE WebCyte2 – An updated Gesneriaceae cytology database.
  • Möller M, Pfosser M, Jang CG, Mayer V, Clark A, Hollingsworth ML, Barfuss MHJ, Wang YZ, Kiehn M, Weber A (2009) A preliminary phylogeny of the ‘didymocarpoid Gesneriaceae’ based on three molecular data sets: Incongruence with available tribal classifications. American Journal of Botany 96(5): 989–1010. https://doi.org/10.3732/ajb.0800291
  • Möller M, Forrest A, Wei YG, Weber A (2011a) A molecular phylogenetic assessment of the advanced Asiatic and Malesian didymocarpoid Gesneriaceae with focus on non-monophyletic and monotypic genera. Plant Systematics and Evolution 292(3–4): 223–248. https://doi.org/10.1007/s00606-010-0413-z
  • Möller M, Middleton D, Nishii K, Wei YG, Sontag S, Weber A (2011b) A new delineation for Oreocharis incorporating an additional ten genera of Chinese Gesneriaceae. Phytotaxa 32(1): 1–36. https://doi.org/10.11646/phytotaxa.23.1.1
  • Möller M, Nishii K, Atkins HJ, Kong HH, Kang M, Wei YG, Wen F, Hong X, Middleton DJ (2016) An expansion of the genus Deinostigma (Gesneriaceae). Gardens’ Bulletin Singapore 68(1): 145–172. http://doi.10.3850/S2382581216000119
  • Nishii K, Hughes M, Briggs M, Haston E, Christie F, DeVilliers JM, Hanekom T, Roos GW, Bellstedt UD, Möller M (2015) Streptocarpus redefined to include all Afro-Malagasy Gesneriaceae: Molecular phylogenies prove congruent with geography and cytology and uncovers remarkable morphological homoplasies. Taxon 64: 1243–1274. https://doi.org/10.12705/646.8
  • Phuong VX (2005) Checklist of plant species in Vietnam. Vol. 3. Agriculture Press, Ha Noi.
  • Puglisi C, Middleton DJ, Triboun P, Möller M (2011) New insights into the relationships between Paraboea, Trisepalum and Phylloboea (Gesneriaceae) and their taxonomic consequences. Taxon 60(6): 1693–1702. https://doi.org/10.1002/tax.606014
  • Sun YX, Moore MJ, Landis JB, Lin N, Chen L, Deng T, Zhang JW, Meng AP, Zhang SJ, Tojibaev KS, Sun H, Wang HC (2018) Plastome phylogenomics of the early-diverging eudicot family Berberidaceae. Molecular Phylogenetics and Evolution 128: 203–211. https://doi.org/10.1016/j.ympev.2018.07.021
  • Thiers B (2016) Index Herbariorum: A Global Directory of Public Herbaria and Associated Staff. New York Botanical Garden’s Virtual Herbarium. http://sweetgum.nybg.org/science/ih/
  • Thiers BM (2019) The world’s herbaria 2018: A summary report based on data from Index Herbariorum. William and Lynda Steere Herbarium, The New York Botanical Garden. http://sweetgum.nybg.org/science/ih/
  • Vasudevan KN (1976) Contribution to the cytotaxonomy and cytogeography of the flora of the western Himalayas: With an attempt to compare it with the flora of the Alps III. Bulletin de la Société Botanique Suisse 8: 152–203.
  • Wang WT, Pan KY, Li ZY (1990) Gesneriaceae. In: Wang WT (Ed.) Flora Reipublicae Popularis Sinicae (Vol. 69). Science Press, Beijing, 141–271.
  • Wang WT, Pan KY, Li ZY, Weitzman AL, Skog LE (1998) Gesneriaceae. In: Wu ZY, Raven PH (Eds) Flora of China (Vol. 18). Science Press, Beijing, & Missouri Botanical Garden Press, St. Louis, 244–401.
  • Weber A, Wei YG, Sontag S, Möller M (2011b) Inclusion of Metabriggsia into Hemiboea (Gesneriaceae), with notes on carpel reduction and its functional background. Phytotaxa 23: 37–48.
  • Weber A, Middleton DJ, Forrest A, Kiew R, Lim CL, Rafidah AR, Sontag S, Triboun P, Wei YG, Yao TL, Möller M (2011c) Molecular systematics and remodeling of Chirita and associated genera (Gesneriaceae). Taxon 60(3): 767–790. https://doi.org/10.1002/tax.603012
  • Weber A, Clark JL, Möller M (2013) A new formal classification of Gesneriaceae. Selbyana 31(2): 68–94.
  • Wei YG, Wen F, Möller M, Monro A, Zhang Q, Gao Q, Mou HF, Zhong SH, Cui C (2010) Gesneriaceae of South China. Guangxi Science and Technology Publishing House, Nanning, Guangxi.
  • Wei YG, Wen F, Zhao B, He SZ (2013) Anna rubidiflora (Gesneriaceae), a new species from Guizhou, the southern part of China. Plant Ecology and Evolution 146(2): 203–211. https://doi.org/10.5091/plecevo.2013.731
  • Wen F, Li S, Xin ZB, Fu LF, Hong X, Cai L, Qin JQ, Pan B, Pan FZ (2019) The updated plant list of Gesneriaceae in China under the new Chinese naming rules. Guangxi Sciences 26(1): 37–63.
  • Yang LH, Feng C, Xu MZ, Sun ZX, Kang M (2019) Synopsis of Cytological Studies on Didymocarpoideae (Gesneriaceae) under New Classification System. Redai Yaredai Zhiwu Xuebao 27(5): 548–557.

Appendix 1

The following is a list of used samples that are ordered alphabetically by taxon with their GenBank accession number of trnL-trnF and ITS sequences respectively. The new taxa whose sequences are newly published are listed with the complete voucher information.

Aeschynanthus rhododendron HQ632895, HQ632993; Aeschynanthus roseoflorus HQ632896, FJ501333; Agalmyla glabra HQ632892, HQ632989; Agalmyla paucipilosa HQ632893, HQ632990; Allocheilos guangxiensis HQ632897, HQ632994; Allostigma guangxiense HQ632880, HQ632977; Anna submontana FJ501542, FJ501362; Briggsiopsis delavayi HQ632879, HQ632976; Cathayanthe biflora HQ632899, HQ632996; Cathayanthe biflora China: Hainan province, Shuiman Town, near Wuzhishan Mountain, WYG180606-01, 6 Jun., 2018, IBK!, MN787055, MN759631; Chayamaritia smitinandii KP325432, KP325425; Codonoboea albomarginata AJ492297, HQ632961; Codonoboea codonion JF912538, JF912565; Codonoboea corrugata FJ501484, HQ632962; Codonoboea elata JF912523, JF912550; Codonoboea floribunda JF912539, JF912566; Codonoboea leucocodon JF912540, JF912567; Codonoboea malayana JF912541, JF912568; Codonoboea pumila JF912543, JF912570; Codonoboea racemosa JF912544, JF912571; Codonoboea venusta JF912545, JF912572; Conandron ramondioides FJ501515, FJ501340; Cyrtandra pendula FJ501530, FJ501354; Cyrtandra pulchella HQ632906, EU919941; Damrongia lacunosa KU203896, KU203801; Damrongia purpureolineata KU203893, KU203798; Deinostigma cyrtocarpa JX506777, JX506885; Didissandra elongata ssp. minor KP325427, KP325420; Didissandra frutescens FJ501522, JN934793; Didissandra sp. KP325429, KP325422; Didymocarpus antirrhinoides FJ501513, DQ912671; Didymocarpus villosus HQ63290, HQ633001; Didymostigma obtusum HQ632875, HQ632971; Didymostigma trichanthera HQ632876, HQ632972; Glabrella mihieri FJ501544, FJ501363; Gyrocheilos chorisepalus var. synsepalus HQ632900, HQ632997; Gyrocheilos lasiocalyx HQ632901, HQ632998; Hemiboea fangii HQ632882, HQ632979; Hemiboea follicularis HQ632885, HQ632982; Henckelia anachoreta 1 HQ632870, HQ632966; Henckelia bifolia JF912522, JF912549; Henckelia dielsii HQ632871, HQ632967; Henckelia floccosa FJ501486, HQ632964; Henckelia grandifolia JF912527, JF912554; Henckelia incana HQ632869, HQ632965; Henckelia longisepala HQ632868, HQ632963; Henckelia pumila 1 JF912529, JF912556; Henckelia pumila 2 FJ501491, FJ501327; Henckelia urticifolia 1 DQ872821, DQ872835; Henckelia urticifolia 2 JF912532, JF912559; Henckelia urticifolia 3 FJ501492, FJ501328; Hexatheca fulva HQ632873, HQ632969; Liebigia barbata FJ501538, DQ912668; Loxostigma glabrifolium HQ632910, HQ633006; Loxostigma griffithii FJ501508, FJ501338; Lysionotus pauciflorus FJ501497, FJ501331; Lysionotus petelotii FJ501496, HQ632974; Metapetrocosmea peltata HQ632872, HQ632968; Microchirita caliginosa FJ501488, FJ501325; Microchirita involucrata 2 JF912526, JF912553; Microchirita mollissima JF912528, JF912555; Microchirita sericea JF912521, JF912548; Microchirita tubulosa JF912531, JF912558; Microchirita viola JF912533, JF912560; Middletonia regularis KU203884, KU203789; Oreocharis dasyantha HQ632918, HQ633014; Oreocharis jiangxiensis HQ632933, HQ633029; Michaelmoelleria vietnamensis VIETNAM. Binh Dinh province, Tay Son district, Tay Giang community, La stream. 13°55'59"N, 108°45'43"E, ca. 148 m, 29 Mar., 2018, WYG180329-01, IBK!, MN787054, MN783373; Oreocharis urceolata HQ632922, HQ633018; Ornithoboea flexuosa KU203931, KU203836; Paraboea clarkei JN934715, JN934757; Petrocodon ainsliifolius HQ632941, HQ633038; Petrocodon dealbatus FJ501537, FJ501358; Petrocodon scopulorum HQ632947, HQ633044; Petrocosmea kerrii FJ501502, FJ501334; Petrocosmea nervosa AJ492299, FJ501335; Primulina gemella FJ501523, FJ501345; Primulina luochengensis HQ632949, HQ633046; Primulina tabacum AJ492300, FJ501352; Pseudochirita guangxiensis HQ632908, HQ633003; Raphiocarpus sinicus HQ632877, HQ632973; Ridleyandra petiolata HQ632935, HQ633032; Ridleyandra quercifolia HQ632936, HQ633033; Somrania albiflora KU203887, KU203792; Spelaeanthus chinii FJ501457, FJ501307; Streptocarpus glandulosissimus KR703972, AF316918; Streptocarpus rexii AJ492305, AF316979; Tribounia grandiflora JX839281, JX839280; Tribounia venosa JX839282, JX839283.

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