Research Article |
Corresponding author: Ren-Bo Zhang ( ddzrb@126.com ) Academic editor: Hanno Schaefer
© 2019 Ren-Bo Zhang, Tan Deng, Quan-Li Dou, Lin He, Xin-Yun Lv, Hong Jiang.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Zhang R-B, Deng T, Dou Q-L, He L, Lv X-Y, Jiang H (2019) Sedum lipingense (Crassulaceae) identifying a new stonecrop species in SE Guizhou, China, based on morphological and molecular evidence. PhytoKeys 134: 125-133. https://doi.org/10.3897/phytokeys.134.38287
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We describe and illustrate Sedum lipingense (Crassulaceae), a new species of stonecrop found in the limestone areas of SE Guizhou, China. Based on the presence of adaxially gibbous carpels and follicles, this taxon belongs to sect. Sedum S.H. Fu. The new species superficially resembles S. subtile Miquel and S. bulbiferum Makino but differs from these two taxa in its development of a basal leaf rosette during florescence. The nrDNA internal transcribed spacer (ITS) sequences also support the claim that this plant is a new species in the Sedum genus.
flora of Guizhou, karst, limestone flora, new taxon, Sedum lipingense
Sedum Linnaeus is the largest genus in the Crassulaceae family, containing about 430 species, with the greatest diversity centering in eastern Asia (
During our fieldwork, a new species of Sedum was discovered in Liping County, Qiandongnan Prefecture, Guizhou Province, China. This particular species has conspicuous rosettes during florescence, an attribute similar to O. balfourii. However, the new species differs from O. balfourii as it possesses central flowering stems rather than lateral ones (Fig.
All morphological characters were measured using dissecting microscopes. Specimen checking was done at PE, IBK, ZY, with the additional use of some web database, including the Plant Photo Bank of China (http://ppbc.iplant.cn/) and Global Plants (http://plants.jstor.org/).
Leaf material from the presumed new species was collected in the field, and immediately dried in silica gel for DNA extraction. The nuclear ribosomal internal transcribed spacer (ITS) regions were used as molecular markers. ITS-F (TGAACCTGCGGAAGGATCAT) and ITS-R (GGTAGTCCCGCCTGACCTG) primers (
The ITS sequence of the new species, as well as the ITS sequences of the congeners downloaded from GeneBank (Table
Accession information relating to internal transcribed spacer (ITS) sequences downloaded from GeneBank.
Species | Voucher | Accession no. |
---|---|---|
Aeonium lancerottense | MEM 1518 | AY082143 |
Aeonium viscatum | MEM 1432 | AY082154 |
Greenovia aizoon | MEM 1425 | AY082112 |
Sedum alfredii | WUK415208 | FJ919953 |
Sedum baileyi | LBG0064555 | FJ919935 |
Sedum bergeri | Ni et al. | AY352897 |
Sedum bulbiferum_416 | Ito416 | LC229234 |
Sedum bulbiferum_hs41 | 130514hs41 | KM111166 |
Sedum bulbiferum_qz09 | 130524qz09 | KM111165 |
Sedum emarginatum | 130512hs27 | KM111145 |
Sedum erici-magnusii | Ito 2077 | LC229235 |
Sedum erythrospermum | Tsutsumi 504 | AB906473 |
Sedum formosanum | Ito 1260 | LC229279 |
Sedum hakonense | S. Mayuzumi C00005 | AB088625 |
Sedum hangzhouense | Ito2604 (TNS) | LC260130 |
Sedum japonicum | Kokubugata 16749 | AB906475 |
Sedum jiulungshanense | CMQ20150076 | LC229243 |
Sedum kiangnanense | Ito 1030 | LC229244 |
Sedum lineare | Mayuzumi C00120 | AB088623 |
Sedum lipingense | ZRB1479 | MN150061 |
Sedum lungtsuanense | Ito3563 | LC260131 |
Sedum makinoi | Kokubugata 16730 | AB906476 |
Sedum mexicanum | Ito 647 | LC229247 |
Sedum morrisonense | Ito2765 | LC229290 |
Sedum multicaule | Miyamoto et al. TI9596136 | AB088631 |
Sedum nagasakianum | Ito2064 | LC229249 |
Sedum nokoense | Kokubugata 10426 | AB906478 |
Sedum oligospermum | CMQ 74 | LC229250 |
Sedum oreades | G. Y. Rao 090803-03 | KF113733 |
Sedum polytrichoides | CMQ1057 | LC229251 |
Sedum rupifragum | Ito 2070 | LC229254 |
Sedum sarmentosum | Ito 978 | LC229255 |
Sedum satumense | Ito2295 | LC229256 |
Sedum trullipetalum | 9420132 | AB088630 |
Sedum subtile_1999 | A. Shimizu 1999 | AB088622 |
Sedum subtile_2259 | Ito2259 | LC229257 |
Sedum subtile_624 | Ito 624 | AB930277 |
Sedum taiwanianum | Ito2770 | LC229297 |
Sedum tetractinum | Ito3623 | LC260135 |
Sedum tianmushanense | LP 67 | LC229261 |
Sedum tosaense | Kokubugata 16726 | AB906483 |
Sedum triactina | 9596091 | AB088629 |
Sedum tricarpum | Ito 2269 | LC229259 |
Sedum trullipetalum | Miyamoto et al.9420132 | AB088630 |
Sedum truncastigmum | Ito3254 | LC229306 |
Sedum yabeanum | S. Mayuzumi C00029 | AB088626 |
Sedum zentaro-tashiroi | H. Ohba 1998 | AB088619 |
In this study, the sequences of 40 species (44 samples) were treated as ingroups. Sequence length was 584 bp for the ITS region, of which 234 characters were constant, 45 characters were parsimony-uninformative and 305 characters were parsimony-informative.
The sequence of the ITS region taken from S. lipingense aligned with the genus Sedum, confirming its generic identity (Fig.
S. lipingense and S. bulbiferum were found to be nested with S. hangzhouense (PP = 41, suggesting a weak support), and then to be nested with S. baileyi and S. makinoi (PP = 100), all species with alternate or opposite stem leaves. Except for S. lipingense, the above four (or perhaps two-three) species were also clustered as a distinct clade (
S. lipingense can be distinguished from the closely related S. subtile and S. bulbiferum by the presence of rosettes, absent sterile shoots and bulbils, subequal lanceolate-oblong sepals, and other traits (Table
Comparing the diagnostics of Sedum lipingense sp. nov., S. subtile and S. bulbiferum.
Traits | S. lipingense | S. subtile | S. bulbiferum | |
---|---|---|---|---|
Rosette leaves during florescence | present | absent | absent | |
Sterile shoots | absent | present | absent | |
Flowering stem | 3–7 cm | 5–10 cm | 7–22 cm | |
Proximal stem leaves | Phyllotaxy | alternate, sometimes opposite on lateral flowering stem | opposite or 3–6-verticillate | opposite |
Leaf blade | broadly obovate | obovate | ovate-spatulate | |
Distal stem leaves | Phyllotaxy | alternate (sometimes subopposite) | alternate | alternate |
Leaf blade | spatulate-oblanceolate | oblanceolate-linear | spatulate-oblanceolate | |
Bulbils in axils | absent | absent | present | |
Cymes | Branches | (2-) 3 | 2- or 3-branched | 3-branched, branches 2-forked |
Branch flowers | 1- to two | 3- to several | many | |
Sepals | lanceolate-oblong, subequal | broadly linear to narrowly lanceolate, unequal | lanceolate to oblanceolate, unequal | |
Nectar scales | broadly cuneate, ca. 0.6 × 0.4 mm, apex truncate | broadly cuneate, ca. 0.4 × 0.5 mm, apex truncate | obovate, ca. 0.6 mm | |
Carpels | ca. 3.5 mm base connate for ca. 1 mm | ca. 5 mm base connate for ca. 2 mm | ca. 4 mm base connate for ca. 1 mm | |
Styles | ca. 1 mm | ca. 2 mm | ca. 1 mm | |
Fl. | Apr–May | Apr–Jun | Apr–May | |
Fr. | May–Jun | Jul–Aug | Jun–Jul |
CHINA. Guizhou Province, Kaili City, Liping County, Mengyan Township, on moist rocks, 26°07'N, 108°42'E, 800 m alt., 13 April 2019, ZRB1479 (fl., holotype ZY!, isotype IBK!), 16 June 2019, ZRB1495 (fr., paratype ZY!)
Biennial (or perennial?) herb. Sterile stems absent. Rosette present during florescence; rosette leaves alternate, broadly obovate, base attenuated and shortly spurred, 0.5–1.5 × 0.4–0.7 cm. Flowering stems 1 to 3 (–4), erect, slender, 3–7 cm; single stems shoot from rosette centers, others shoot from the rosette leaf axils; lateral proximal leaves sometimes opposite, akin to rosette leaves but smaller, 0.6–0.8 × 0.3–0.5 cm, base shortly spurred; distal leaves alternate, spatulate-obovate to spatulate-oblanceolate, 0.7–1.2 × 0.3–0.4 cm, apex obtuse, base shortly spurred. Cymes scorpioid, 2 to 3 branched; branches 1 to 2 flowered; bracts obliquely oblanceolate, apex obtuse, 4–9 × 2–4 mm. Sepals 5, lanceolate-oblong, subequal, ca. 2 mm, base shortly spurred, apex obtuse. Petals 5, yellow, broadly lanceolate, ca. 4 mm, apex mucronate. Stamens 10; antesepalous one ca. 3 mm; antepetalous one inserted ca. 1 mm above petal base, slightly shorter than the antesepalous stamens. Nectar scales broadly cuneate, ca. 0.6 × 0.4 mm, apex truncate. Carpels erect, lanceolate, ca. 3.5 mm, base connate for ca. 1 mm. Styles slender, ca. 1 mm. Follicles stellately divergent at maturity. Seeds oblong, ca. 0.6 mm, papillate.
At this time, based on our field observations, Sedum lipingense is only known to occur in Longxi village, Mengyan town, Liping County, Guizhou Province. It grows on moist limestone rocks, at ca. 800 m altitude, in groups of several hundred individuals.
This species is currently known to occur in a single valley and we suggest its placement in the Data Deficient category of
This new species was observed flowering from April to May and fruiting from May to June.
The specific epithet ‘lipingense’ is derived from the plant’s locality, Liping County, Guizhou Province, China.
This work was supported by grants from the Doctor Foundation of Zunyi Normal College (BS[2018]17), the Science and Technology Bureau of Zunyi City – Zunyi Normal College Foundation Joint Project ([2018]11), the Innovation Ability Promotion Plan of Guizhou Higher School (QJHXTCXZ [2013]11).