Corresponding authors: Yi-Gang Wei (
Academic editor: Clifford Morden
Fu L-F, Xiong C, Monro AK, Fan Q, Chen Z-X, Wen F, Xin Z-B, Wei Y-G, Liao W-B (2022)
A recent systematic study has demonstrated that
While conducting field investigations into the Danxia flora of Guangdong, China, we encountered an unknown species of
All morphological characters were observed from field and herbarium specimens using an Olympus SZX16 binocular microscope (Japan). For achene morphology, we also undertook scanning electron micrograph (
A species conservation assessment was undertaken for the new species described here using IUCN criteria (
Leaf material for DNA extraction was dried using silica gel (
Clean reads were paired and imported in Geneious Prime (
We generated a phylogeny using sequences data from previous phylogenies of
The complete plastid genome and ribosomal DNA (
Plastid genome map of
Summary of whole plastid genome of
Characteristic |
|
---|---|
Size (bp) | 151,857 |
LSC length (bp) | 82,836 |
SSC length (bp) | 18,407 |
IR length (bp) | 25,307 |
Number of genes | 113 |
Protein-coding genes | 79 |
rRNA genes | 4 |
tRNA genes | 30 |
LSC GC% | 34.30% |
SSC GC% | 30.70% |
IR GC% | 42.80% |
Genes encoded in plastid genome of
Group of genes | Gene name |
---|---|
tRNA genes |
|
rRNA genes |
|
Ribosomal small subunit |
|
Ribosomal large subunit |
|
DNA-dependent RNA poly merase |
|
Photosystem I |
|
Photosystem II |
|
Large subunit of rubisco |
|
NADH dehydrogenase |
|
Cytochrome b/f complex | |
ATP synthase |
|
Maturase | |
Subunit of acetyl-CoA carboxylase |
|
Envelope membrane protein |
|
Protease | |
Translational initiation factor |
|
C-type cytochrome synthesis |
|
Conserved open reading frames |
|
Note: Genes with one or two introns are indicated by one (*) or two asterisks (**), respectively. Genes in the IR regions are followed by the (× 2) symbol.
The characteristics and statistics of the datasets used in this study are summarized in Table
Statistics for the molecular datasets used in this study.
Number of sequences | Aligned length (bp) | Variable characters (bp) | Parsimony information characters (bp) | Model used | |
---|---|---|---|---|---|
ITS | 142 | 528 | 419 | 339 | - |
|
139 | 677 | 667 | 38 | - |
|
84 | 637 | 318 | 315 | - |
Combined | 142 | 1,842 | 1,404 | 692 | GTR+GAMMA |
Phylogenetic tree of
China. Guangdong: Danxiashan National Park, Renhua County, Shaoguan City,
Most similar to
Herbs prostrate, stem 30–200 × 1 mm, light green when fresh, drying yellowish-brown, glabrous, succulent, cystoliths fusiform, 0.2–0.4 mm long. Stipules, 1.3–1.5 × 1.7–2.1 mm, reniform, drying brown, papery, with dense cystoliths. Leaves petiolate, distichous, clustered towards the stem apex; petioles 2–8 mm long, glabrous, cystoliths densely scattered; laminae at each node equal or subequal, 3–15 × 5–20 mm, length: width ratio 0.7–0.9:1, suborbicular to broadly ovate, succulent, papery when dry; adaxial surface drying grey-green, dark green when fresh, glabrous, with cystoliths densely scattered,
Flowering from March to May, fruiting from April to June.
The species epithet is named after the Danxia landform with which the species is associated.
dān xiá lěng shuǐ huā (Chinese pronunciation); 丹霞冷水花 (Chinese name).
At present,
China. Guangdong: Danxiashan National Geopark, Renhua County, Shaoguan City,
Plate of
Within
Diagnostic comparison of
Characters |
|
|
|
---|---|---|---|
Stipule shape and length | reniform, 1.3–1.5 mm | triangular, |
triangular, |
Petiole length | 2–8 mm | 0.2–0.6 mm | 3–20 mm |
Staminate peduncle length | 8–25 mm | 1.5–7 mm | 2–5 mm |
Pistillate tepal number | 3 | NA | 2 |
This study describes a new species of
We would like to thank the staff at IBK, IBSC, K, PE and SYS who provided help for checking specimens. We are also grateful to Ms. Liao Li-Juan for participating in fieldwork. This work was supported by the Guangdong Provincial Special Research Grant for the Creation of National Parks (2021GJGY034), the National Natural Science Foundation of China (grant number 31860042), and the Light of West China Program of the Chinese Academic of Sciences ([2020]82).
Appendix S1
GenBank accession numbers.
Taxa and GenBank accession numbers of DNA sequences used in this study.