Corresponding authors: Natalia V. Shchegoleva (
Academic editor: M. Pellegrini
New data on the phylogeny of four rare and endemic species of
Shchegoleva NV, Nikitina EV, Juramurodov IJ, Zverev AA, Turginov OT, Jabborov AM, Yusupov Z, Dekhkonov DB, Deng T, Sun H (2022) A new species of
More than 90 species of
The morphological features indicated that the unknown plants belonged to
The unknown plants closely resembled
Morphological observations and measurements of
DNA isolation was performed using a Plant Genomic DNA Kit (TIENGEN Biotech, Beijing, China) according to the manufacturer’s protocol. CTAB extraction protocol with some modifications was used to extract genomic DNA from herbarium specimens of
Selected nuclear DNA regions ITS1-ITS2 (for herbarium specimen
Primers used in this study.
Primer name | Sequences (forward / reverse) | DNA fragment size, bp | Primer source |
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ITS1-18S ITS4-26S | 5’-TCCGTAGGTGAACCTGCGG-3’ 5’-TCCTCCGCTTATTGATATGC-3’ | ~ 700 bp |
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ITS1 | 5’-TCCGTAGGTGAACCTGCGG -3’ | ~ 650 bp |
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ITS2 | 5’-GCTGCGTTCTTCATCGATGC-3’ | ||
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5’-CGATCTATTCATTCAATATTTC-3’ 5’-TCTAGCACACGAAAGTCGAAGT-3’ | ~ 900 bp |
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5’-CGAAATCGGTAGACGCTACG -3’ 5’-ATTTGAACTGGTGACACGAG-3’ | ~ 900 bp |
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5’-ATGTCACCACAAACAGAGACTAAAGC-3’ 5’-GTAAAATCAAGTCCACCRCG-3’ | ~ 600 bp |
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To obtain sequences of the genes of interest, PCR amplification was carried out according to the following parameters (except
PCR amplification for
To determine the taxonomic status and systematic position of
Accession numbers of samples used for phylogenetic analyses of
Species | GenBank accession number | |||
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Sequence alignments were performed using ClustalW (
Phylogenetic trees were reconstructed using Maximum Likelihood (
The phylogenetic tree, based on the nuclear and plastid sequences (Fig.
Bayesian tree based on combined nuclear (
The results of the phylogenetic analysis were similar to the results of
It is obvious that
Similar to
Uzbekistan. Hissar Ridge, Baysuntau, Khodzha-Gurgur-ata Mountains, vicinity of the Village Gumatag,
Series of basal leaves in related species
Uzbekistan. Pamir-Alay, South-western spurs of the Hissar Ridge, Baysuntau, vicinity of the Village Gumatag, amongst the stones, 4 June 2013, O. Turginov (TASH-003754).
Flowering in May. Fruiting in May and June.
Distribution of
Comparison of
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rounded-ovate, 3–5-dissected, segments further 2–5-dissected, lobules elongated, rounded at apex | triangular-reniform, 3–5-partite, segments narrowly cuneate, unequally and subacutely dentate | reniform, dissected 1/3 to nearly 1/2 of its length, lobes broad incised-dentate | broadly ovate, dissected into pinnatipartite segments, segments tripartite, terminal lobules oblong |
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narrowly elliptic, concave, less than half as long as petals, with long reclinate hairs | elliptic, concave, some shorter than the petals, with sparse, long reclinate hairs | elliptic, concave, half as long as petals, with short reclinate spreading hairs | elliptic, concave, with scattered hairs |
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oblong-obovate, greenish-yellow, base cuneate, margin undulate | obovate, bright yellow, becoming dark when dry, base broadly cuneate, margin undulate | very broadly ovate, golden yellow, base cuneate, margin slightly undulate | oblong-ovate, yellow-green, base narrowly cuneate, marginundulate |
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1.0–1.8 mm long, asymmetrically ovate, slightly convex, with semi-appressed hairs | 2.2–2.5 mm long, oblong, slightly laterally compressed, with appressed hairs | 1.8–2.5 mm long, oblong, slightly convex, with appressed hairs | 1.5–2.0 mm long, asymmetrically obovate, laterally compressed, with scattered not appressed hairs |
1 | Blades of basal leaves broadly ovate, dissected into pinnatipartite segments, with tripartite-oblong terminal lobules |
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– | Radical leaves 3–5-dissected or lobed-incised |
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2 | Blade of radical leaves 1/3 or nearly 1/2 unequally partite into broad incised-dentate lobes |
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– | Blade of radical leaves 3–5-dissected |
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3 | Blade of basal leaves triangular-reniform, 3–5-dissected almost to the base, wedge-shaped segments, unequally sharp-toothed |
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– | Blade of radical leaves is round-reniform, 3–5-dissected, each section divided into 2–5 elongated lobules; apex of lobules rounded |
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This study was supported by the International Partnership Program of the Chinese Academy of Sciences (151853KYSB20180009), the framework of the Second Tibetan Plateau Scientific Expedition and Research (STEP) program (2019QZKK0502), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA20050203), the Key Projects of the Joint Fund of the National Natural Science Foundation of China (U1802232), the Youth Innovation Promotion Association of the Chinese Academy of Sciences (2019382), Yunnan Young & Elite Talents Project (YNWR-QNBJ-2019-033), the Chinese Academy of Sciences “Light of West China” Program, the state research project “Taxonomic revision of polymorphic plant families of the flora of Uzbekistan” (FZ-20200929321), the State Programs for 2021–2025 years “Grid mapping of the flora of Uzbekistan” and “The creation of DNA bank and barcoding of endemic plants of Uzbekistan”, as well as in accordance with the current State Assignment of Central Siberian Botanical Garden at Siberian Branch of Russian Academy of Sciences (AAAA-A21-121011290026-9).