Appendix 1
List of GenBank accession numbers and vouchers for newly published sequences for all sequences used in the analyses. Data are arranged in the following order: taxon name in bold (in alphabetical order); voucher data (country, estate, locality, collecting date, collector, collecting number, rpl16, psbA-trnH).
Coryphantha durangensis Britton & Rose, HM041405, AY545338; C. hesteri Y. Wright, AY545234, AY545342; C. pallida Britton & Rose, AY545232, AY545340; C. vivipara Britton & Rose, KC196809, KC196847; Escobaria chihuahuensis Britton & Rose, AY545233, AY545341; E. zilziana (Boed.) Backeb., AY545236, AY545344; Ferocactus haematacanthus (Salm-Dyck) Borg ex Backeb., HM041431, MH129870; F. latispinus Britton & Rose, HM041432, MH129871; Mammillaria albicans A. Berger, AY545238, AY545346; Mammillaria albilanata subsp. albilanata Backeb., México, Guerrero, 10 km east of Huitzuco, 31 October 2018, Cristian Cervantes, CC047, MT995687, MT995715; Mammillaria albilanata subsp. oaxacana D. R. Hunt, México, Oaxaca, Santiago Huauclilla-Santa Catarina Tlaxila road near the river, 1 October 2017, Cristian Cervantes, CC036, MT995698, MT995726; México, Oaxaca, 1.1 km from the junction of highway 135 Teotitlán de Flores Magón-San Fransisco Telixtlahuaca heading to San Sebastián Sedas, 2 October 2017, Cristian Cervantes, CC040, MT995689, MT995717; México, Oaxaca, Santa Maria Jalapa de Marquéz towards the microwave antenna, 22 June 2018, Cristian Cervantes, CC044, MT995678, MT995706; México, Oaxaca, 3.8 Km from Santo Domingo Tonalá to San Agustín Atenango, 24 June 2018, Cristian Cervantes, CC046, MT995677, MT995705; Mammillaria albilanata subsp. reppenhagenii (D. R. Hunt) D. R. Hunt, México, Jalisco, Tolimán, -, -, Hilda Arreola, s.n., MT995702, MT995730; Mammillaria albilanata subsp. tegelbergiana (H. E. Gates ex G. E. Linds.) D. R. Hunt, México, Chiapas, Comitán de Dominguez – 3.1 km from Comitán, 19 January 2007, Salvador Arias, SA1630, -, -; M. armillata K. Brandegee, AY545240, AY545349; M. bachmannii Boed., AY545241, AY545350; M. backebergiana Franc. G. Buchenau, AY545242, AY545351; M. barbata Engelm, AY545243, AY545352; M. beneckei Ehrenb., AY545244, AY545353; M. blossfeldiana Boed., AY545245, AY545354; M. bombycina Quehl & Quehl, AY545246, AY545356; M. boolii G. E. Linds., AY545247, AY545357; M. brachytrichion Lüthy, AY545248, AY545358; M. cadereytensis R. T. Craig, AY545249, AY545359; M. candida Scheidw., AY545250, AY545360; M. carnea Zucc. ex Pfeiff., HM041449, AY545363; M. cerralboa Orcutt, AY545254, AY545364; M. columbiana subsp. columbiana Salm-Dyck, Venezuela, Mérida, -, 2012, Teresa Terrazas, TT957, -, -; M. columbiana subsp. yucatanensis (Britton & Rose) D. R. Hunt, México, Yucatán, Municipio Dzemul, 2004, CICY, G. Carnevali & I. M. Ramírez, 7449, MT995701, MT995729; M. subsp. crucigera Mart., México, Oaxaca, 2 km from San Antonio Nahuahautipan, 9 August 1990, Ulises Guzmán, UG787, MT995697, MT995725; M. crucigera subsp. tlalocii (Repp.) D. R. Hunt, México, Oaxaca, 8 km from the rural road Santa María Tecomavaca-Santa María Ixcatlán, 9 November 1994, Ulises Guzmán, UG1103, MT995696, MT995724; M. decipiens Scheidw., AY545255, AY545369; M. dixanthocentron Backeb. ex Mottram, México, Oaxaca, km 107 highway 135 between Tecomavaca and Cuicatlán, 24 Octuber 1990, Ulises Guzmán, UG828, MT995679, MT995707; M. elongate DC., AY545258, AY545373; M. eriacantha Link & Otto ex Pfeiff., México, Veracruz, km 305 of the Xalapa-Veracruz highway between Plan del Río and Cerro Gordo, 21 January 2012, Salvador Arias, SA2169, MT995700, MT995728; M. flavicentra Backeb. ex Mottram, México, Oaxaca, Teotitlan de Flores Magón 10 Km on the way to Huautla, 13 July 1995, Patricia Novoa, CPNL132, MT995688, MT995716; M. formosa Scheidw., AY545259, AY545376; M. fraileana (Britton & Rose) Boed., AY545260, AY545377; M. gasseriana Boed., AY545261, AY545378; M. geminispina DC., AY545262, AY545379; M. glassii R. A. Foster, AY545263, AY545380; M. grusonii Runge, AY545266, AY545383; M. guelzowiana Werderm., AY545267, AY545384; M. haageana Pfeiff., México, Puebla, 1 km over the gap from the junction with the Puebla-Xalapa highway, 15 May 2017, Cristian Cervantes, CC020, MT995686, MT995714; México, Veracruz, 7 km from Perote, 15 May 2017, Cristian Cervantes, CC021, MT995693, MT995721; México, Puebla, 1.5 km south of Esperanza, 16 May 2017, Cristian Cervantes, CC022, MT995692, MT995720; México, Puebla, 7 km west of Tehuacan, 16 May 2017, Cristian Cervantes, CC023, MT995675, MT995703; México, Puebla, near the Helia Bravo Botanical Garden, 16 May 2017, Cristian Cervantes, CC024, MT995680, MT995708; México, Puebla, 9.5 km from junction 125 to Huajolotitlán towards Los Reyes Metzontla, 16 May 2017, Cristian Cervantes, CC025, MT995676, MT995704; México, Oaxaca, km 59.5 Highway 125 then join the road to San Sebastián Frontera, 17 May 2017, Cristian Cervantes, CC027, MT995683, MT995711; México, Veracruz, In La Organera area near the Tecamalucan town, 29 September 2017, Cristian Cervantes, CC029, MT995685, MT995713; México, Oaxaca, 26 Km on the Huauclilla-El Parian dirt road, 1 Octuber 2017, Cristian Cervantes, CC035, MT995690, MT995718; México, Oaxaca, 11.5 km from Magdalena Jaltepec heading to Santiago Tilangongo, 1 Octuber 2017, Cristian Cervantes, CC039, MT995691, MT995719; México, Oaxaca, 7.5 km from Corral de Piedra to Santa Maria Tutla, 22 June 2018, Cristian Cervantes, CC045, MT995684, MT995712; México, CDMX, in the Reserva Ecológica del Pedregal de San Ángel (REPSA), 6 December 2018, -, -, MT995681, MT995709; M. halei K. Brandegee, AY545269, AY545386; M. hernandezii Glass & R. A. Foster, AY545270, AY545387; M. herrerae Werderm., AY545271, AY545388; M. huitzilopochtli subsp. huitzilopochtli D. R. Hunt, México, Oaxaca, 7 km northwest of San Juan Bautista Cuicatlán, 5 August 1990, Salvador Arias, SA856, MT995694, MT995722; M. huitzilopochtli subsp. niduliformis (A.B.Lau) Pilbeam, México, Oaxaca, Río Santo Domingo up the junction Rio Salado, 12 March 1983, A. B. Lau, ABL1495, MT995695, MT995723; M. humboldtii Ehrenb., AY545273, AY545390; M. insularis H. E. Gates ex Shurly, AY545275, AY545392; M. jaliscana Boed., AY545276, AY545393; M. karwinskiana Mart., AY545277, AY545394; M. klissingiana Boed., AY545278, AY545395; M. lanata Orcutt, México, Puebla, Rio Hondo cerca del puente Calapa autopista Tehuacán-Oaxaca, 19 November 1994, Patricia Novoa, CPNL122, MT995699, MT995727; M. lasiacantha Engelm., AY545279, AY545396; M. lindsayi R. T. Craig, AY545280, AY545398; M. longimamma DC., AY545281, AY545399; M. luethyi G. S. Hinton, AY545282, AY545400; M. magnifica Franc. G. Buchenau, AY545283, AY545401; M. magnimamma Haw., AY545284, AY545402; M. mainiae K. Brandegee, AY545285, AY545403; M. mammillaris H. Karst., AY545286, AY545404; M. mazatlanensis K. Schum., AY545287, AY545407; M. melanocentra subsp. rubrograndis (Repp. & A. B. Lau) D. R. Hunt, AY545288, AY545408; M. mercadensis Patoni, AY545289, AY545410; M. microhelia Werderm., AY545291, AY545411; M. moelleriana Boed., AY545292, AY545412; M. mystax Mart., AY545294, AY545414; M. nazasensis (Glass & R. A. Foster) Repp., AY545295, AY545416; M. neopalmeri R. T. Craig, AY545296, AY545417; M. oteroi Glass & R. A. Foster, AY545297, AY545418; M. parkinsonii Ehrenb., AY545298, AY545419; M. patonii Werderm. in Backeb., AY545299, AY545420; M. pectinifera F. A. C. Weber, AY545300, AY545421; M. peninsularis Orcutt, AY545301, AY545422; M. pennispinosa Krainz, AY545302, AY545423; M. perezdelarosae Bravo & Scheinvar, AY545303, AY545424; M. phitauiana Werderm. in Backeb., AY545305, AY545426; M. picta Meinsh., HM041452, AY545427; M. plumosa F. A. C. Weber in Bois, AY545307, AY545428; M. polyedra Mart., AY545308, AY545429; M. pondii Greene, HM041399, AY545431; M. poselgeri Hildm., HM041400, AY545432; M. pottsii Scheer ex Salm-Dyck, AY545312, AY545433; M. prolifera (Mill.) Haw., AY545313, AY545434; M. rekoi Vaupel, AY545314, AY545435; M. rettigiana Boed., AY545315, AY545436; M. rhodantha Link & Otto, AY545316, AY545437; M. schumannii Hildm., AY545317, AY545438; M. senilis Lodd. ex Salm-Dyck, AY545318, AY545440; M. sinistrohamata Boed., AY545319, AY545441; M. sphacelate Mart., AY545320, AY545442; M. spinosissima Lem., AY545321, AY545443; M. stella-de-tacubaya Heese, AY545322, AY545444; M. supertexta Mart. ex Pfeiff., México, Oaxaca, 0.5 km east of San Juan de los Cues, 9 August 1990, Ulises Guzmán, UG793, MT995682, MT995710; M. tetrancistra Engelm., KC196805, KC196840; M. thornberi subsp. thornberi Orcutt, AY545324, AY545447; M. thornberi subsp. yaquensis (R. T. Craig) D. R. Hunt, AY545325, AY545448; M. vetula subsp. gracilis (Pfeiff.) D. R. Hunt, AY545327, AY545449; M. voburnensis subsp. voburnensis Scheer, AY545328, AY545450; M. voburnensis subsp. eichlamii (Quehl) D. R. Hunt, AY545329, AY545451; M. weingartiana Boed., AY545330, AY545452; M. wrightii Engelm., AY545331, AY545454; M. zacatecasensis Shurly, AY545332, AY545455; M. zephyranthoides Scheidw., AY545333, AY545457; Neolloydia conoidea Britton & Rose, HM041462, AY545458; Ortegocactus macdougallii Alexander, HM041484, AY545459; Pelecyphora aselliformis C. Ehrenb., AY545336, AY545460; Stenocactus lloydii A. Berger, AY545337, AY545461.