Corresponding author: Hong Liu (
Academic editor: A. Freire-Fierro
Fu L-F, Yang T-G, Lan D-Q, Wen F, Liu H (2021)
The latest revision of Chinese
Previous studies have demonstrated that
As part of ongoing research into the diversity of Chinese
All morphological characters were studied based on the material from field and herbarium specimens using a dissecting microscope (SMZ171, Motic, China). For seed morphology, we also undertook scanning electron micrograph (
To confirm the systematic position of this unknown species, we conducted phylogenetic studies using
Species names and GenBank accession numbers of
Species | Location | Voucher specimens | Herbarium | Genbank number |
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Nikkou-shi, Japan | HSN09815 | HSN |
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Gangwon, South Korea | KYI-2009032 | – |
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Shanxi, China | HZ2017050107362 | HSN |
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Sichuan, China | HSN013113 | HSN |
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Sichuan, China | HSN06442 | HSN |
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Hunan, China | SZ2016080907105 | HSN |
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Nikkou-shi, Japan | HSN09817 | HSN |
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Nikkou-shi, Japan | HSN09823 | HSN |
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Nikkou-shi, Japan | HSN09816 | HSN |
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Nikkou-shi, Japan | HSN7797 | HSN |
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Sichuan, China | JZ2018042507981 | HSN |
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Sichuan, China | QCS2017102608035 | HSN |
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Nikkou-shi, Japan | HSN09810 | HSN |
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Shanxi, China | HSN7760 | HSN |
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Hunan, China | HSN7505 | HSN |
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Hubei, China | HSN09188 | HSN |
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Zhejiang, China | HSN7909 | HSN |
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Shimane-ken, Japan | DG2019032310004 | HSN |
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Nikkou-shi, Japan | HSN09818 | HSN |
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Anhui, China | BD2017030507343 | HSN |
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Jilin, China | HSN7379 | HSN |
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Hubei, China | BD2017030507344 | HSN |
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Jilin, China | CBS2016062406656 | HSN |
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Nikkou-shi, Japan | HSN09820 | HSN |
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Yunnan, China | GLGH20170607375 | HSN |
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Gansu, China | HSN07772 | HSN |
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Nikkou-shi, Japan | HSN09819 | HSN |
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Sichuan, China | HSN7980 | HSN |
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Jilin, China | SJH2017052107372 | HSN |
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Jilin, China | SJH2017052107371 | HSN |
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Hunan, China | TPS2017042407504 | HSN |
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Shanxi, China | HSN7761 | HSN |
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Tibet, China | HSN7380 | HSN |
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Shanxi, China | HSN13356 | HSN |
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Hunan, China | TPS2017042307449 | HSN |
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Shimane-ken, Japan | DG2019032310003 | HSN |
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Kanagawa, Japan | – | – |
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Gifu, Japan | – | – |
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Nagasaki, Japan | – | – |
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Saitama, Japan | – | – |
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Iowa, USA | – | – |
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Wales, UK | – | – |
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Northwest Territories, Canada | – | – |
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Nunavut, Canada | Brysting_01-065_CAN | CAN |
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Yukon, Canada | Bennett_08-125_CAN | CAN |
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Hatfield, New Hampshire, USA | – | – |
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Chile | – | – |
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Hunan, China | ZJ2016031506369 | HSN |
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Anhui, China | HSN07355 | HSN | ||
– | – | – |
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– | – | – |
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We performed phylogenetic analyses of
We conducted the
Living plants of the new species were cultivated in the green house of South-Central University for Nationalities. Actively growing root tips were harvested after 1–2 weeks. Cytological examination was performed following
The aligned matrix of
Phylogenetic tree of
The chromosome number of
Somatic chromosomes at metaphase of
Similar to
Illustration of
Morphological comparison of
Characters |
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from all leaf axils | from near stem base | from all leaf axils | from all leaf axils | only from basal leaf axils |
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absent | absent | absent | absent | present |
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2–3 pairs, 10–30 × 10–25 mm | 1–3 pairs, 9–13 × 10–14 mm | 2–7 pairs, 4–17 × 4–17 mm | 3–5 pairs, 3–18 × 5–18 mm | 1–2 pairs, 6–10.5 × 7.5–11.5 mm |
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square | unknown | rounded | rounded | square |
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erect | spreading | erect | erect | erect |
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absent | distinct | somewhat inconspicuous | absent | absent |
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lobes conspicuous unequal | lobes conspicuous unequal | lobes conspicuous unequal | lobes subequal | lobes conspicuous unequal |
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red brown, papillose | dark brown, papillose | dark brown, papillose | red brown, smooth | dark brown, papillose |
China. Hunan: Badagongshan National Nature Reserve, Sangzhi County,
Perennial herbs, 10–25 cm tall.
Plate of
sāng zhí jīn yāo (Chinese pronunciation); 桑植金腰 (Chinese name).
At present,
Macro- and micro-morphology of
Our phylogenetic analysis is consistent with previous studies (
The basic chromosome number of Japanese
In this study, we confirm and describe a new species of
This work was supported by the construction plan of Hubei province science and technology basic conditions platform (No.2017BEC014), Fund for key laboratory construction of Hubei province (No.2018BFC360) and the major projects of technological innovation in Hubei province (2019ABA101).