Chromosome preparations fixed in 3:1 EtOH – acetic acid and stained with orcein, objective lens: 100×. a Mitotic plate of a male C. grandis (2n = 22 + XY) b Mitotic plate of a male plant of C. hirtella (2n = 24) c Phase contrast image of a mitotic plate of a male plant of C. sessilifolia (2n = 24).