Corresponding author: Kiyotaka Hori (
Academic editor: Th. Almeida
I describe
Hori K (2020) Deparia × nanakuraensis K.Hori (Athyriaceae), a new hybrid pteridophyte from Japan. PhytoKeys 165: 69–84.
The genus
The genus is characterized by hair-like scales and disconnected grooves between rachises and costae (
In Japan, several hybrids of the genus
The
In this study,
Additionally, specimens from the Collection Database and Materials of TNS (
For the conservation assessment, the area of occupancy (
To observe mitotic chromosomes, root tips were collected in the field, and pre-treated with 0.004 M 8-hydroxyquinoline for 6 h at approximately 17–20 °C. After fixation in ethanol and acetic acid (3:1) for 15–30 min, the root tips were hydrolyzed in 1 N HCl at 60 °C for 1–3 min and then squashed in 2% aceto-orcein solution. The chromosomes were observed under a microscope (Leica DM2500) and then photographed by using a digital camera (Leica MC170 HD).
For the molecular analyses, total DNA was extracted from silica-dried leaves using cetyltrimethylammonium bromide solution, according to
Gel electrophoresis of
The accession numbers of DNA sequences in the datasets were shown in Appendix
Mitotic metaphase chromosome number observed in an individual of
Photograph and sketch of mitotic metaphase chromosomes (2
We sequenced 653–746 bp of the
The
The
The
Japan. Honshu: Akita prefecture, Noshiro city, Futatsui town, Nanakura-shrine,
The name derives from Nanakura-shrine, Futasui town, Noshiro City, Akita prefecture, northeast Japan, where
Map showing the known distribution of
Wild plant of
Juvenile of
Indefinite growth through bud (red arrow) on rhizome of
IUCN Red List Category. Based on estimates from GeoCAT, the
Abaxial surface of pinnule and sori of
Morphological comparison among
Characteristics | Shape of frond | Shape of sori | Margin of indusium | Base of pinnule | Serration of pinnules | Apex of pinnules |
---|---|---|---|---|---|---|
|
deltoid-ovate or ovate-lanceolate | reniform to U-shaped | serrated | with costal wing | rounded | acute |
|
deltoid | oblong to J-shaped | entire to serrated | with narrow costal wing | rather rounded | rather acute |
|
oblong | oblong to J-shaped | entire | truncated to costa | truncate | obtuse |
The ploidy level of this hybrid is the same as its parents because
In conclusion, this study described
I am grateful to Mr. Y. Horii for plant collections. This study was supported by a Grant-in-Aid for JSPS Fellows 18K14785 to K. H.