Academic editor: Norbert Holstein
He Q, Zhao R, He A, Zhu Z, Tong Y (2021) Taxonomic Identity of
Specimens of
In the protologue,
Differences between
Species | Leaf | Length × width (cm) | Margin of leaf | Nutlet |
---|---|---|---|---|
|
Elliptic-lanceolate or oblong-lanceolate | 4–8 × 1.5–2.5 | Doubly setiform serrate | Pubescent |
|
Lanceolate or ovate-lanceolate | 2.5–4.5 × 1–1.5 | Simply setiform serrate | Only villous at apex |
When revising the species of
Specimens of
Specimens used for measurement of morphological characters of
Species | Collector | Collection number | Collection site | Herbarium |
---|---|---|---|---|
|
T. L. Dai | 104469 | Chengkou, Chongqing | PE |
H. F. Zhou | 26421 | Fengjie, Chongqing | KUN | |
H. F. Zhou | 26317 | Fengjie, Chongqing | KUN | |
Z. R. Zhang | 25054 | Fengjie, Chongqing | KUN | |
Y. Liu | 668 | Shennongjia, Hubei | KUN | |
P. C. Cai | 20297 | Shimen, Hunan | CSFI | |
C. L. Long | 87290 | Shimen, Hunan | CSFI | |
J. R. Zheng | 80108 | Shimen, Hunan | CSFI | |
P. C. Cai | 20442 | Zhangjiajie, Hunan | CSFI | |
P. Y. Li | 8277 | Langao, Shaanxi | KUN | |
T. P. Soong | 39145 | Baoxing, Sichuan | KUN | |
G. H. Yang | 57149 | Emeishan, Sichuan | KUN | |
W. P. Fang | 7546 | Emeishan, Sichuan | PE | |
W. P. Fang et al. | 31110 | Emeishan, Sichuan | IBK | |
W. P. Fang et al. | 32888 | Emeishan, Sichuan | IBSC | |
G. H. Yang | 54569 | Emeishan, Sichuan | IBSC | |
S. G. Wu | 394 | Sichuan | KUN | |
G. R. Chen | 2383 | Tiantai, Zhejiang | KUN | |
Anonymous | 2759 | Without precise locality | KUN | |
S. Y. Hu | 1906 | Without precise locality | KUN | |
|
Y. T. Xiao | 40700 | Tianmenshan, Hunan | CSFI |
K.W. Liu | 33359 | Tianmenshan, Hunan | CSFI, PE | |
Q. P. Zhang | 2020072801 | Tianmenshan, Hunan |
|
|
Q. P. Zhang | 2020072802 | Tianmenshan, Hunan |
|
Twelve individuals from five populations of the two species (Table
Geographical information of four populations of
Species | Number of individuals | Collection site | Latitude (N) / Longitude (E) | Altitude (m) |
---|---|---|---|---|
|
4 ( |
Hupingshan, Hunan |
|
350 |
3 ( |
Dapanshan, Zhejiang |
|
600 | |
1 ( |
Liujiaping, Hubei |
|
1380 | |
1 ( |
Caoyuan, Guizhou |
|
1460 | |
|
3 ( |
Tianmenshan, Hunan |
|
1335 |
DNA was extracted using the modified CTAB method (
ITS sequences used in this study are from NCBI; a dash (–) indicates missing data.
Species | Location | Voucher | ITS from NCBI |
---|---|---|---|
Hupingshan, Hunan | Z.F. Chen 20200601 |
|
|
Hupingshan, Hunan | Z.F. Chen 20200603 |
|
|
Hupingshan, Hunan | Z.F Chen 20200605 |
|
|
Hupingshan, Hunan | Z.F. Chen 20200607 |
|
|
Dapanshan, Zhejiang | A.G. He 20200710 |
|
|
Dapanshan, Zhejiang | A.G. He 20200715 |
|
|
Dapanshan, Zhejiang | A.G. He 20200717 |
|
|
Liujiaping, Hubei | Z.Q. Lu |
|
|
Caoyuan, Guizhou | Z.Q. Lu |
|
|
Tianmenshan, Hunan | Q.P. Zhang 2020072801 |
|
|
Tianmenshan, Hunan | Q.P. Zhang 2020072802 |
|
|
Tianmenshan, Hunan | Q. P. Zhang 2020072803 |
|
|
|
Xingshan, Hubei | Chen et al. 961325 |
|
– | Liu 631 |
|
|
Tiantai Mt., Zhejiang | – |
|
|
Qinling Mt. | SZH 454 |
|
|
Qinling Mt. | Q875 |
|
|
|
Daba Mt. Shaanxi | – |
|
|
Daba Mt. Shaanxi | – |
|
|
Hangzhou Bot. Gard., Zhejiang | W 97-30 |
|
– | Lee s. n. |
|
|
Qinling Mt. | HZ 283 |
|
Re-collections of material from the type localities and further field investigation showed that the leaf shapes are quite variable in the same area, from ovate-lanceolate to lanceolate, then narrow-lanceolate (Fig.
Measurements of morphological characteristics of
Species | Specimen | Leaf length × width (cm) | Leaf size ratio | Leaf margin | Infructescence length (cm) | Bract size (cm) | Nutlet indumentum |
---|---|---|---|---|---|---|---|
|
T. L. Dai 104469, PE00818275 | 5–5.9 × 1.7–1.8 | 2.9–3.3 | Simply setiform serrate | 2.6–2.9 | 1 × 0.35 | Villous at apex |
H. F. Zhou 26421, KUN0590808 | 5.1–5.9 × 1.9–2.3 | 2.5–2.8 | Simply setiform serrate | 3.2–5.6 | 1.3 × 0.5 | Villous at apex | |
H. F. Zhou 26317, KUN0590809 | 5.5–6.2 × 1.7–1.9 | 3–3.4 | Simply setiform serrate at apex, doubly setiform serrate in the rest | 1.8–3.2 | 1.2 × 0.45 | Villous at apex | |
Z. R. Zhang 25054, KUN0590804 | 5.8–6.5 × 2.1–2.5 | 2.3–3.1 | Simply setiform serrate | 4.4–4.9 | 1.5 × 0.6 | Villous at apex | |
Y. Liu 668, KUN0590794 | 3.9–4.1 × 1.2–1.3 | 3–3.3 | Simply setiform serrate | 2.1 | 1 × 0.3 | Pubescent | |
P. C. Cai 20297, CSFI017457 | 5.3–6 × 1.8–2.1 | 2.8–3 | Doubly setiform serrate | 2.6–3.9 | 1.2 × 0.4 | Pubescent | |
C. L. Long 87290, CSFI017461 | 5.4–6.1 × 1.6–2.2 | 2.5–3.4 | Doubly setiform serrate | 3.9–4.3 | 1.2 × 0.5 | Villous at apex | |
J. R. Zheng 80108, CSFI017456 | 6.7–7.1 × 2.3–2.5 | 2.8–3 | Doubly setiform serrate | – | – | – | |
P. C. Cai 20442, CSFI017455 | 5.1–5.7 × 1.8–1.9 | 2.7–3 | Simply setiform serrate at apex, doubly setiform serrate in the rest | 2.2–2.7 | 1.2 × 0.5 | Villous at apex | |
P. Y. Li 8277, KUN0590814 | 5.9–7.3 × 2.2–2.7 | 2.4–3 | Simply setiform serrate | 4.4–4.8 | 1.4 × 0.6 | Villous at apex | |
T. P. Soong 39145, KUN0590798 | 5.5–6.5 × 2.4–2.7 | 2.2–2.7 | Doubly setiform serrate | 2–3.6 | 1.2 × 0.4 | Villous at apex | |
G. H. Yang 57149, KUN0590811 | 6–7.4 × 2.1–2.5 | 2.8–3 | Simply setiform serrate, sometimes doubly setiform serrate | 4.1–5.5 | 1.4 × 0.5 | Pubescent | |
W. P. Fang 7546, PE00818253 | 5–6.2 × 1.8–2.5 | 2.5–2.8 | Simply setiform serrate | 2.8 | 1.2 × 0.5 | Villous at apex | |
W. P. Fang et al. 31110, IBK00079496 | 6.7–7.5 × 1.9–2.1 | 3.2–3.7 | Simply setiform serrate | 3.1 | 1 × 0.3 | Pubescent | |
W. P. Fang et al. 32888, IBSC0368122 | 5.4–6.6 × 2.5–3 | 2–2.3 | Doubly setiform serrate | 3.2 | 1.5 × 0.5 | Villous at apex | |
G. H. Yang 54569, IBSC0368131 | 6–7.2 × 2–2.5 | 2.4–3.3 | Doubly setiform serrate | 3.9 | 1.4 × 0.5 | Villous at apex | |
S. G. Wu 394, KUN0590803 | 4.8–5.6 × 1.5–1.8 | 2.7–3.2 | Simply setiform serrate | 2.2–3.5 | 1.1 × 0.4 | Villous at apex | |
G. R. Chen 2383, KUN0590815 | 5.1–5.8 × 1.7–1.9 | 2.8–3 | Doubly setiform serrate | 2.8–4.1 | 1.1 × 0.4 | Pubescent | |
Anonymous 2759, KUN0590800 | 5.7–6.4 × 1.8–2.1 | 2.9–3.5 | Doubly setiform serrate | 3.1 | 1.3 × 0.4 | Pubescent | |
S. Y. Hu 1906, KUN0590797 | 6–6.6 × 2–2.2 | 2.7–3 | Simply setiform serrate, sometimes doubly setiform serrate | 5–6.5 | 1.5 × 0.6 | Villous at apex | |
|
Y. T. Xiao 40700, CSFI017539 (paratype of |
5.1–5.7 × 1.2–1.5 | 3.4–3.8 | Simply setiform serrate | 3.5–4.2 | 1.1 × 0.35 | Villous at apex |
Y. T. Xiao 40700, CSFI017465 (paratype of |
5.7–6.5 × 2.3–2.8 | 2.2–2.7 | Doubly setiform serrate | 3.6–3.8 | 1 × 0.4 | Villous at apex | |
K.W. Liu 33359, CSFI050241 (holotype of |
4.3–4.7 × 1.4–1.6 | 2.9–3.6 | Simply setiform serrate | 2.5 | 0.7 × 0.4 | Pubescent | |
K. W. Liu 33359, PE01843387 (isotype of |
4.4–5 × 1.3–1.7 | 2.8–3.5 | Simply setiform serrate | 2.1 | 0.7 × 0.4 | Pubescent | |
Q. P. Zhang 2020072801, |
5–5.6 × 1.6–1.8 | 2.7–3.3 | Simply setiform serrate | – | – | – | |
Q. P. Zhang 2020072802, |
4.5–4.9 × 1.4–1.8 | 2.7–3.7 | Simply setiform serrate, sometimes doubly setiform serrate | 2 | 0.9 × 0.3 | Villous at apex |
Nutlet trichomes of
Phenetic comparison of ITS sequences showed that the samples of
The neighbor-joining (
We therefore conclude that
A deciduous tree, up to 15 m tall. Bark grey. Branchlets slender, dark purplish, covered with white roundish lenticels, densely villous and soon glabrous. Petiole 5–11 mm, 0.5–0.8 mm in diameter; leaf blade ovate-lanceolate to narrow-lanceolate, sometimes oblong or ovate, 2.5–8 × 1–3 cm, base broadly sub-rounded or slightly cordate, margin doubly setiform serrate, sometimes simply setiform serrate at the apex or simply setiform serrate, upper surface sparsely villous, densely villous along the veins, glabrescent, lower surface densely villous along the veins, sometimes bearded in axils of lateral veins, lateral veins 15–20 on each side of the mid-vein. Infructescence 1.4–6.5 cm long, pendent, peduncle slender, densely villous with white roundish lenticels; bracts semi-ovate-lanceolate, 5–15 × 3–6 mm, outer margin dentate, without basal lobe, inner margin entire with small, inflexed basal auricle. Nutlets broadly ovoid, 2–4 × 1.5–3 mm, villous at apex, glabrous or sparsely villous or pubescent on the remaining part, ribbed.
This work was supported financially by the National Natural Science Foundation of China (31770752) and the Fifth Stage Funded Research Projects of 333 in Jiangsu Province (BRA2018065). The authors are grateful to the curators of many herbaria (CSFI, HIB, IBK, IBSC, KUN, NAS, P, PE and WUK) for permission to access and study the specimens, as well as the staff of Central South University of Forestry and Technology for providing the holotype photos of