Representative agarose gels where PCR products were amplified with the primers ISSR F5 (highest number of polymorphic bands, top) and ISSR F5 (lowest level of polymorphic bands, down). 1 Serratula tinctoria 2 Klasea quinquefolia 3 K. oligocephala 4 K. kotschyi 5 K. serratuloides 6 K. erucifolia 7 K. lasiocephala 8 K. cerinthifolia 9 K. grandifolia 10K. radiata subsp. radiata11 K. hakkiarica 12 K. haussknechtii 13 K. coriaceae 14K. radiata subsp. radiata15 K. kurdica 16 K. bornmuelleri 17 Centaurea ptosimopappoides 18 C. straminicephala 19 Jurinea cataonica, M: marker.