Corresponding author: Nian-He Xia (
Academic editor: E. Ruiz-Sanchez
Li X, Ni J-B, Xia N-H (2023) The identity of
However, the previous molecular phylogenetic analysis (
During the field trip to Baishi Town, Yunfu City, Guangdong Province in July 2021, one bamboo species with oblong foliage leaves was found. It matched the isotype very well and shares the same key characters, such as the slightly prominent culm supranodal ridge, the white powdery infranodal region, the glabrous internodes with three branches at an upper node, the solitary secondary branch, three to six foliage leaves clustered at the top of ultimate branches, the small-medium-sized and oblong foliage leaves with glabrous blades and conspicuous transverse veins. Therefore, we are certain that the specimens we collected are
The sample of
For obtaining reliable results, a reasonable proof strategy with two steps was designed to identify the systematic position of
Young leaves at the vegetative growth stage were collected in the field. Total genomic DNA was isolated from silica-dried leaves following the manufacturer’s specifications TIANGEN Genomic DNA Extraction Kit (TIANGEN, Beijing, China). DNA samples of concentration up to standard (≥1 μg) were sheared into fragments using Covaris M220 (Covaris, Woburn, MA). Insert size of 350 bp fragments were enriched by PCR, and the paired-end (2 × 150 bp) libraries were constructed on NovaSeq 6000 platform. About 20G deep genome skimming (
The filtered clean reads were utilized to de novo assemble complete chloroplast (
List of 24 species with species names, voucher information and GenBank accession numbers for the plastid tree based on eight combined plastid sequences extracted from whole chloroplast genomes (WCG).
Taxon | Voucher information | GenBank accession | ||||||||
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WCG | atpI/H | psaA-ORF170 | rpl32-trnL | rpoB-trnC | rps16-trnQ | trnD/T | trnS/G | trnT/L | ||
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Zhang 07067 (KUN) | / |
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Li & Zhang 07051 (KUN) | / |
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Zeng & Zhang 06081 (KUN) | / |
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Zhang 07013 (KUN) | / |
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Zhang 07021 (KUN) | / |
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Triplett 223 (KUN) | / |
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Zeng & Zhang 06055 (KUN) | / |
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Triplett 228 (KUN) | / |
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Triplett 146 (KUN) | / |
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Triplett 133 (KUN) | / |
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Triplett 126 (KUN) | / |
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Zhang 08006 (KUN) | / |
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CZY56 (IBSC) |
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XNH187 (IBSC) |
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NH028 (IBSC) |
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NH032 (IBSC) |
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BH58 (IBSC) |
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BH124 (IBSC) |
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CZY163 (IBSC) |
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Not provided by the author |
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MPF10034 (KUN) |
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DZL09122 (KUN) |
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Not provided by the author |
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zmy018 (KUN) |
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After referring to previous plastid phylogeny studies of
The latest high-quality genome sequence of moso bamboo (
For a reliable phylogenetic tree based on SNP dataset, we considered that filtered raw
Chloroplast DNA regions and SNP dataset were utilized to reconstruct the phylogenetic tree, respectively. Eight plastid matrices were aligned using MAFFT v 7.450 (
To make clear the position of
Comparison of
Morphology |
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Nearly solid, with appressed branches | Hollow, with patent branches |
Intravaginal, glabrous abaxially | Transferrd, setose abaxially and readily deciduous when old | |
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Ciliate on the upper | Ciliate wholly |
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Arched, with ciliolate margin | Truncate, with glabrous margin |
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3–6, with irregular arrangement clustered at the top ultimate branch | 4–7, with a coplanar arrangement at the top ultimate branch |
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Glabrous abaxially, 1–4 mm long per adjacent sheath apex | Hirsute abaxially, 5–13 mm long per adjacent sheath apex |
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7–10 × 1.5–2.6 cm, with 6–7-paired secondary veins | 12.5–25 × 2.5–3.2 cm, with 7–9-paired secondary veins |
China. Guangdong: type locality unknown, cultivated in Bamboo Garden, Sun Yat-sen University, 5 April 1980,
Isotypes of
Shrubby bamboo, rhizomes leptomorph. Culm erect, 1–1.5 m tall, 2–7 cm in diameter; branches appressed, usually 1 branch at the lower culm nodes and 2–3 at the mid or upper nodes of culm (if 3 branches, central slightly dominant than lateral); internodes terete or slightly flattened above branches, 5–27 cm long, glabrous, nearly solid; nodes flat or slightly prominent, white powdery under nodes; supranodal ridge flat or slightly prominent; intranodes 5–8 mm high, glabrous; culm buds solitary, ovate to elliptic, light yellow, puberulent abaxially at upper part, ciliolate on the upper margin, apex obtuse. Culm leaf sheath persistent or deciduous later, intravaginal, thinly leathery, 1/3–2/3 as long as internodes, glabrous abaxially, ciliate on the upper margin and sometimes glabrescent, longitudinal ribs conspicuous; sheath scar with remains of sheath base; auricles falcate to long-elliptic, obliquely ascending, 2–4 × 1–2 mm; oral setae erect or slightly curved, 3–10 mm long; ligule entire, 0.5–1 mm high, asperous abaxially, ciliolate on the margin, apex arched; blades narrowly lanceolate to lanceolate, erect, glabrous abaxially, margin sparsely serrate. Foliage leaves 3–6 clustered at the top of ultimate branches, with irregular arrangement; sheath thinly leathery, glabrous abaxially, margins densely ciliate, sometimes glabrous, thinly white-powdery, longitudinal ribs conspicuous, length per adjacent sheath apex very short, 1–4 mm; auricles undeveloped, ovate to falcate or absent, 1–3 × 1–1.5 mm; oral setae erect or curved, 5–10 mm long, usually deciduous when old; inner ligule 0.5–1 mm high, densely ciliolate on margin, apex truncate; outer ligule ca. 0.5 mm high, ciliolate on margin; blades oblong to oblong-lanceolate, papyraceous, 7–10 × 1.5–2.6 cm, glabrous adaxially and abaxially, apex acute to attenuate, base obtuse to unequal rounded, margin serrate; secondary veins 6–7 pairs, tertiary veins 6–7 pairs, transverse veins conspicuous; petioles 2–4 mm long, glabrous; Inflorescence unknown.
New shoots were produced from March to July.
Jǔ Yè Shǐ Zhú (Chinese pronunciation); 矩叶矢竹 (Chinese name).
Phylogenetic relationships of
Phylogenetic relationships of
However, previous studies (
We thank Dr. Qiang Fan for hosting our visits to the Bamboo garden of Sun Yat-sen University and Zhuo-yu Cai for taking some photos of type specimens of
SNP matrix
phylogenetic
This SNP matrix contains 14 species with 36490 bp.