Examples of ACO gene sequence concatenations for selected Spiranthes. Samples 135–140 labeled “S. ×kapsheviakii” represent a priori interior lowland S. sheviakii from Hough and Young (2021), all other samples are from Pace and Cameron (2017). Samples 141 & 142 represent Appalachian S. × kapnosperia. Samples of S. cernua s.s. are included immediately above the highlighted box and samples of S. ochroleuca are included immediately below the highlighted box A examples of ambiguities in a priori S. sheviakii that correspond to nucleotide differences between S. cernua and S. ochroleuca (e.g., G, R, A) B examples of nucleotide states that are shared with S. ochroleuca but not S. cernua (e.g., left-most highlighted A & G), and additional examples of ambiguous states in a priori S. sheviakii that correspond to nucleotide differences between S. cernua and S. ochroleuca (e.g., G, R, and A).