Research Article |
Corresponding author: Ebru Doğan Güner ( ebrudogang@gmail.com ) Academic editor: Alan Paton
© 2016 Ebru Doğan Güner.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Güner ED (2016) Oenanthe incrassans: An enigmatic species from Turkey and its comparison with Oenanthe pimpinelloides (Apiaceae). PhytoKeys 62: 101-111. https://doi.org/10.3897/phytokeys.62.8106
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Oenanthe incrassans (Apiaceae) was discovered in Istanbul, Turkey. It is related to Oenanthe pimpinelloides, but it clearly differs in terms of leaves, inflorescence (ray, bracts, and bracteoles) and fruit features. A taxonomic description, some photographs of the species, geographical distribution and habitat features are given. Additionally, fruit micromorphology, stem, ray and fruit anatomy, and pollen features are studied for the first time and compared to Oenanthe pimpinelloides.
Anatomy, micromorphology, pollen, Oenanthe , taxonomy, Turkey
Oenanthe incrassans Bory & Chaubert (
Foley and Southam also discussed Oenanthe thracica Griseb. which is the other synonym of Oe. pimpinelloides (Hedge & Lammond, 1972). They said that Oe. thracica is conspecific with Oe. pimpinelloides and its taxonomy is in need of further study. Their result was based on examination of a specimen of Oe. thracica recorded as “Turkey (European)–A1(E) Edirne: Kesan, 6 July 1982, Nydegger 17003”. In 2013, Özhatay et al. erroneously reported Oe. incrassans as a new record for Flora of Turkey based on this record (
Within the scope of revisionary studies on the Oenanthe species in Turkey, numerous field trips were held between 2014–2015, on one of which Oe. incrassans was discovered in Istanbul. Additionally, W and
This study aims to present a full description of the species and resolve the delimitation between Oenanthe incrassans and Oe. pimpinelloides by comparing morphological, anatomical, palynogical and micromorphological analyzes and their ecological features.
The specimens of Oenanthe incrassans and Oe. pimpinelloides were collected in different regions of Turkey between 2014–2015 and checked with relevant literature (
Pollen acquired from anthers of the herbarium specimens were prepared based on Wodehouse method, stained with basic fuchsin, and analyzed under light microscope (
≡Oe. incrassata Bory & Chaub. in Chaub. & Bory, Nouv. Fl. Pelop.: 19. 1838
Bory & Chaub., Exp. Sci. Moreé, Bot.: tab. 8. 1835. (designated by
GREECE. Crete: Listr. Malevyzi, in paludosis fluviorum Gazanos et Almyros prope Gazi, 25 June 1942, KH. Rechinger fil. 14050 (W!); Sphakia: Sumpfiger Badem bei Frankokasteli, 13 April 1904, I.Dörfler, (
TURKEY. Istanbul: c. 35 km NW von Istanbul, bei Durusu, am Ufer des Durusu–Sees, 20 m s.m., 41°17'43"E/ 28°35'40"N, 16 May 2000, E. Vitek 2000–28 (W!); Terkos to Karaburun, 20–50 m, marshy lakeside, 30 May 2014, ED. Güner 2009 (
Perennial, 50–70 cm tall, herb, glabrous, with ovoid or oblong tubers far from stem base. Stem erect, sparsely branched above, hollow, deeply striate (furrowed). Basal and lower stem leaves 2–pinnate, ovate to lanceolate in outline, up to 15 cm with petiole; ultimate segments with pinnatifid lobes, ovate, 9–15 × 8–14 mm; petiole shorter than leaf lamina, broader at leaf base. Upper stem leaves 2–pinnate, ovate-triangular in outline; ultimate segments 2–2.5 cm long and 2–5 mm broad, elliptic. Umbels with 7–12 rays of subequal length (1.5–2 cm), rays becoming hardly thickened and elongating in fruit; involucral bracts 0–1, linear, up to 6×1 mm. Umbellets almost flat, with unequal, thickened pedicels in fruit, many flowered, about 1.5 cm diam., pedicel of surrounding flowers longer than inner ones. Bracteoles 10–12, linear, ca. 3 × 1 mm. Petals radiating, creamy white, the outer flowers are female, petals cordate, deeply emerginate in tip, inner petal surface papillate. Styles shorter than fruit, fruit oblong, 3.5–4 × 2–2.5 mm.
Oenanthe incrassans is distributed in Greece and Turkey (Figs
Oenanthe incrassans is close to Oe. pimpinelloides, but it clearly differs in leaves, inflorescens and features of fruit. Their differences are given in Table
Comparison of the morphological characters of Oenanthe incrassans and Oe. pimpinelloides.
Oenanthe incrassans | Oe. pimpinelloides | |
---|---|---|
Ultimate segments of basal and lower stem leaves | Pinnatifid, ovate, 9–15 × 8–14 mm | Pinnatilobate or pinnatifid, ovate–triangular, 8–10 × 5–8 mm |
Ultimate segments of upper stem leaves | Elliptic, 20–25 × 2–5 mm | Linear or narrowly elliptic, 30–35 × 0.4–1.5 mm |
Rays and pedicels | Strongly thickened | Thickened |
Bracts | 0–1 | 0–3 |
Bracteoles | 10–12, ca 3 × 1 mm | 12–14, 1.5–2 × 0.5 mm |
Sepals | 0.4–0.9 mm in fruit | 0.2–0.4 mm in fruit |
Styles | Shorter than fruit | ± Equal fruit body |
Fruit | 3.5–4 × 2–2.5 mm | 2.5–3 × 1–1.5 mm |
Stem anatomy: The shape of stem cross section is triangular or ovoid in outline in Oenanthe incrassans; whereas it is circular in Oe. pimpinelloides. Parenchymatic cells of cortex 4–5–seriate in Oe. incrassans; but it is 2–4–seriate in Oe. pimpinelloides. Sclerenchyma tissue cells are 4–5–seriate between two peripheral vascular bundles in Oe. incrassans; while they are 10–12–seriate in Oe. pimpinelloides. 1–2 small central bundles are placed below peripheral bundles in Oe. incrassans; but 1–3 central bundles are placed below peripheral bundles in Oe. pimpinelloides (Fig.
Rays are hardly thickened and the shape of cross section is 8–10–ridged and circular in outline in Oenanthe incrassans; but they are slightly thickened and 7–ridged ovoid or oblong in outline in Oe. pimpinelloides. There are 8–9–seriate collenchyma cells in Oe. incrassans; but 5–6 seriate in Oe. pimpinelloides. Pith cells are 3–4–seriate and disappear towards the center in Oe. incrassans; but they are present at the center in Oe. pimpinelloides (Fig.
Size and shapes of mericarps show morphological differences between the two species. The cross section shape of mericarps is semi-circular in outline and 4–ridged at the dorsal surface in Oenanthe incrassans. However, it is triangular in outline and only faintly 4-ridged in Oe. pimpinelloides. Mesocarp tissue consists of two types of cells; parenchymatic-slightly thickened cells and lignified sclerenchyma cells around vascular bundles. There are 9–10–seriate parenchymatic cells in Oe. incrassans, but there are 4–5– seriate parenchymatic cells in Oe. pimpinelloides (Fig.
Oenanthe incrassans and Oe. pimpinelloides show fruit characteristics of the genus Oenanthe. The fruit micromorphology of Oe. incrassans differs from Oe. pimpinelloides by 3.5–4 × 2–2.5 mm sized mericarps (not 2.5–3 × 1–1.5 mm); sepals 0.4–0.9 mm in fruit (not 0.2–0.4 mm); styles shorter than fruit body (not ± equal fruit body); pedicel width ± equal fruit body width (not narrower). While lateral ridges of mericarp are 0.7–0.9 mm width in Oe. incrassans, it is 0.5–0.6 mm width in Oe. pimpinelloides. Stylopodium is conical and embedded along calyx line in both species (Fig.
The pollen grains characters of Oenanthe incrassans and Oe. pimpinelloides are given in the Table
Detailed comparison table of pollen features of Oenanthe incrassans and Oe. pimpinelloides.
Oenanthe incrassans (EDG 2009) | Oenanthe pimpinelloides (EDG 2028) | |
---|---|---|
Symmetry | Radial, isopolar | Radial, isopolar |
Pollen shape (P/E) | Perprolate (P/E = 2.09) | Prolate (P/E = 1.89) |
Equatorial outline | Elliptic | Elliptic |
Ornamentation | Rugulate (equatorial area), perforate (polar area) | Rugulate(equatorial area), perforate (polar area) |
Exine sculpturing | Subtectate | Subtectate |
Polar Axis (P) (min-max) | 43.2±0.41 mm (37 mm–47 mm) | 31.2±0.43 mm (26 mm–36 mm) |
E1 = Equatorial Axis (wide side of polen) (min-max) | 21.0±0.27 mm (15 mm–22.5 mm) | 14.8±0.22 mm (12 mm–17 mm) |
E0 = Equatorial Axis (center of pollen) (min-max) | 20.6±0.26 mm (15.5 mm–22 mm) | 16.5±0.24 mm (13 mm–19 mm) |
E2 = Equatorial Axis (narrow side of polen) (min-max) | 20.6±0.26 mm (15.5 mm–22 mm) | 16.5±0.24 mm (13 mm–19 mm) |
Clg = Colpus length (min-max) | 33.9±0.44 mm (27 mm–36 mm) | 24.3±0.41 mm (20 mm–29 mm) |
Clt = Colpus width (min-max) | 2.1±0.04 mm (1.5 mm–2.5 mm) | 1.9±0.04 mm (1.5 mm–2.5 mm) |
Plg = Pore length (min-max) | 5.3±0.12 mm (3 mm–6 mm) | 4.9±0.10 mm (4 mm–6 mm) |
Plt = Pore width (min-max) | 5.0±0.07 mm (3 mm–6 mm) | 4.9±0.12 mm (4 mm–7 mm) |
Int-e = Intine equatorial (min-max) | 1.1±0.01mm (1 mm–1.25 mm) | 0.9±0.02 mm (0.75 mm–1 mm) |
Int-p = Intine polar (min-max) | 1.0±0.01 mm (1 mm–1.25 mm) | 0.7±0.03 mm (0.50 mm–1 mm) |
Ex-e = Exine equatorial (min-max) | 1.1±0.02 mm (1 mm–1.25 mm) | 0.9±0.02 mm (0.75 mm–1 mm) |
Ex-p = Exine polar (min-max) | 1.0±0.01 mm (0.75 mm–1.25 mm) | 0.7±0.03 mm (0.50 mm–1 mm) |
There are many studies about anatomical features of genera of Apiaceae (
Oenanthe pimpinelloides shows wide distribution in the World and also in Turkey. The species has been recorded Aegean, Mediterrenean, Thrace and Black Sea region in Turkey but not the East Anatolian region (Figure
I would like to thank TUBITAK for its financial support (Project number KBAG–114Z005). Also I would like to thank Mustafa Erdem for language check of the manuscript.
Selected Specimens Examined. Oenanthe pimpinelloides: TURKEY. Edirne: Keşan, Murat Köy Dam Lake, road sides, steppe, 90 m, 19 June 2014, 40°45"5.8'N / 26°45"5.6'E, ED. Güner 2047 & B. Bani (