Research Article |
Corresponding author: Özlem Çetin ( ozlemcetin8419@gmail.com ) Academic editor: Peter de Lange
© 2015 Özlem Çetin, Meryem Öztürk Şeker, Ahmet Duran.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Çetin Ö, Şeker MÖ, Duran A (2015) A new subspecies of Seseli gummiferum (Apiaceae) from Ilgaz Mountain National Park, northern Turkey. PhytoKeys 56: 99-110. https://doi.org/10.3897/phytokeys.56.5755
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A new subspecies Seseli gummiferum subsp. ilgazense A.Duran, Ö.Çetin & M.Öztürk, subsp. nov. (Apiaceae) is described from Kastamonu province, Turkey. It was collected from the open Pinus sylvestris L. and Abies nordmanniana (Steven) É.Spach. mixed forest in the northern Anatolian region. An endemic apparently confined to the Ilgaz Mountain National Park, the new taxon is closely related to Seseli gummiferum subsp. gummiferum. Diagnostic morphological characters for closely similar taxa are discussed, and a key to the subspecies of Seseli gummiferum is presented. ITS (Internal Transcribed Spacer) region of the nuclear ribozomal DNA of closely related Seseli L. taxa and Pimpinella is used to constract phylogenetic tree by using BioEdit and Seaview Programme.
New taxon, Seseli gummiferum , Turkey, Umbelliferae
The Apiaceae comprise approximately 450 genera and 3700 species worldwide (
Seseli L. is one of the largest genera in the Apiaceae with 125 to 140 taxa ascribed to the genus worldwide. Represented by both intraspecific and interspecific diversity, Seseli is distributed in Europe, Asia, Africa, North America and Australia (
In 2008, during a field trip in the Black Sea region of Turkey, an unusual specimen of Seseli was collected by the authors. Study of the descriptions in
DNA isolation: Total DNA was obtained from 50–75 mg leaf and fruit from six different individuals. DNAs are isolated with CTAB method and after concentrations were determined by Nanodrop. Sample DNAs were diluted 25 ng/µl. Stok DNAs were kept at -86 °C.
ITS amplifications: ITS region of studied taxa were amplified using ITS4 (5' TCC TCC GCT TAT TGA TAT GC 3') and ITS5 (5' GGA AGG AGA AGT CGT AAC AAG 3') primers. PCR condition is 95 °C for 5 min initial denaturation, 35 cycles of 94 °C for 30 s denaturation, 50 °C for 30 s anneling, and 72 °C for 1 min extension, 72 °C for 10 min final extension.
Data collection and cluster of phylogenetic analysis: PCR products were visualised by agarose jel. The amplified fragments were sequenced using the same primers used for amplification. ITS sequences of the taxa were aligned via Bioedit and were used to construct phylogenetic trees by using Seaview.
Seseli gummiferum gummiferum Pall. ex Sm., Exot. Bot. [Smith] ii, 121 (1807).
Replaced synonym: Bubon rigidus Spreng., Syst. Veg. (ed. 16) [Sprengel] 1: 900 1825 [1824], nom. illeg., non Bubon rigidus (Waldst. & Kit.) Spreng. Pl. Min. Cogn. Pug. 2: 53. 1815. Type: [London] Cult. in Oxford Bot. Garden and Hort. Lady Hume.
Basionym: Seseli crithmifolium Boiss., Fl. Orient. [Boissier] 2: 962 (1872). Type: Greece, Insulae maris Aegei, Tournefort 324 (holotype P, photo!, E!).
Affinis S. gummiferum subsp. gummiferum sed umbellis centralibus radiis 13–21 (nec 25–30), bracteis 2–7 (nec plerumque 8–15), umbellis lateralibus radiis 7–13 (nec 15–20), ovariis glabris (nec cum pilis), fructibus glabris (nec cum pilis) differt.
TURKEY. A4 Kastamonu: Ilgaz Mountain Natural Park, Kastamonu road, from Çatören village to Büyük Hacet Hill, 6 km, in open Pinus sylvestris L. and Abies nordmanniana (Stev.) Spach. mixed forest, serpentine stony slopes, 41°06'344"N, 33°48'628"E, 1465 m, 22 August 2008, A.Duran 8135, Ö.Çetin & M.Öztürk (holotype
Plants monocarpic, 15–30 cm tall; rootstock thickened, cylindrical, oblong, ± vertical, 8–15 mm diameter; stems 3 to 5, terete, finely ridged, mostly glabrous, sparsely puberulent above, with a developed fibrous collar 2.5–7 cm; stems mostly branching from the base, below and rarely at the middle part, green to purplish green. Basal leaves ovate to oblong-ovate, 2-pinnate, 6–20 × 3–10 cm, glabrous; ultimate segments lanceolate, linear-oblong, 5–15 × 0.5–1.5 mm, acute to acuminate; sheaths developed, distinctly widened at base, upper sheath surface sulcate, margin membranous; cauline leaves similar to basal leaves, partly reduced, amplexicaule, middle and upper portions of the stem leafless. Flowers hermaphroditic; the central umbel stout, 7–11 cm diam., equal to or longer than lateral umbels, rarely shorter, with 13 to 21 rays, 1.8–5.5 cm long, puberulent above, unequal, each central umbel with by 2 to 7 bracts; bracts lanceolate, rarely widened at base, 10–17 mm long, margin ± membranous, sometimes bifid to trifid, glabrous or puberulent; umbellules 9–15 mm diam, each comprising 55 to 70 flowers; bracteoles 15–20, connate at base, 5–8 mm long, linear- lanceolate, acuminate, entire or bifid to trifid, green to partly purple, margin distinctly membranous, especially ciliate at apex, out side glabrous to puberulent; lateral umbels 3.5–6 cm diam. with 7 to 13 rays, 1–3.5 cm long, with bracts or without. Flowers subsesile, glabrous, sepals ±purplish, broadly lanceolate, glabrous, persistent, ca. 1 mm; petals white, glabrous with deflexed apex; filament white, anther clearly purple; ovary glabrous. Fruit oblong-elliptic, 3.5–4 × 1.5–2 mm, glabrous, with 5 ribs prominent, obtuse; stylopodium short-conical; styles deflexed in fruit, distinctly purple.
Cross section of fruit: emb = embriyo; dvb = dorsal vascular bundle; ex = exocarp; cdv = central dorsal vittae; es = endosperm; lvb = lateral vascular bundle; ldv = lateral dorsal vittae; ae = aerenchyma; cv = commissural vittae; cvb = carpophore vascular bundle; covb = commissural vascular bundle. Scale bar = 0.2 mm.
The new subspecies is known only from the type locality, with specimens collected only from Ilgaz Mountain National Park (Kastamonu province) in Turkey, where the species seems to be very rare. This area is ca. 1.5 km2, and mature individuals of the type population number approximately 125. The location is very close to the road side and near forest management. The population is going to be negatively affected from cars, trucks and people in the future. The habitat of this subspecies is clearly under threat of destruction, and therefore, the taxon should be considered Critically Endangered (CR), according to IUCN Red List Criteria (
This new subspecies grows at 1450–1470 m with Lapsana communis L., Abies nordmanniana (Stev.) Spaach, Centaurea drabifolia Sm., Erysimum thyrsoideum Boiss., Pteridium aquilinum (L.) Kuhn, Sorbus umbellata (Desf.) Fritsch, Valeriana alliariifolia Adams, Eryngium giganteum M.Bieb., Bupleurum falcatum subsp. persicum (Boiss.) Koso-Pol., Salvia verticillata L., Teucrium chamaedrys L., Dactylis glomerata L., Asyneuma rigidum subsp. rigidum.
Turkey is the most complex country in the Middle East with regard to geographic structure and landforms. It’s comprised of comparatively narrow and long, variously oriented mountain chains, separated by deep valleys and also high- and medium-elevational plateaus. The geological composition and physical direction, exposure and altitude of these mountains are here largely influential not only upon the diversity of vegetation, but also on the richness of the flora (
Seseli gummiferum subsp. ilgazense is closely related to two other subspecies found in Turkey. S. gummiferum subsp. gummiferum is distributed in Crimea, Central Anatolia (
Seseli gummiferum subsp. ilgazense also differs from S. gummiferum subsp. crithmifolium (DC.) P.H.Davis, which is distributed in west and east Crete, Folegandros, Sikinos, Amargos, Karpathos, Saria and neigbouring islands in Aegean Sea. The new taxon is not so broadly distributed, endemic and found only in northern Anatolia (Ilgaz Mountain). Seseli gummiferum subsp. crithmifolium principally differs from subsp. ilgazense by its puberulent fruits, the central umbels with 20–45 rays, and the oblong leaf segments (6–) 10–30 × 2–5 mm (
1 | Fruit glabrous; the central umbel 13 to 21 rays; lateral umbels with 7 to 13 rays | subsp. ilgazense |
– | Fruit with hairs; the central umbel 22 to 70 rays; lateral umbels with 13 to 47 rays | |
2 | Basal leaves glabrous | subsp. crithmifolium |
– | Basal leaves puberulent or finely pubescent | subsp. gummiferum |
Since
Diagnostic characters of Seseli gummiferum subsp. ilgazense with the three related taxa are provided in Table
Diagnostic characters of Seseli gummiferum subsp. ilgazense, S. gummiferum subsp. gummiferum, S. gummiferum subsp. crithmifolium and S. corymbosum.
Characters | subsp. ilgazense | subsp. gummiferum | subsp. crithmifolium | S. corymbosum |
---|---|---|---|---|
Stems per plant | 3 to 5 | 1 to 4 | solitary | solitary |
Stem branching | mostly below, rarely in middle portion | below the middle | above the middle | above the middle |
Central umbel rays | 13 to 21 | 25 to 30 | 22 to 45 | 30 to 70 |
Lateral umbel rays | 7 to 13 | 15 to 20 | 17 to 35 | 13 to 47 |
Bracts | 2 to 7 | mostly 8 to 15 | mostly absent, rarely up to 2 | 0 to 1 |
Bracteoles | 15 to 20 | 11 to 16 | 19 to 26 | 19 to 23 |
Petals | glabrous | glabrous | pubescent | pubescent |
Ovary | glabrous | pubescent | puberulent | pubescent |
Fruit | glabrous | pubescent | pubescent | pubescent |
Five Seseli taxa and Pimpinella were evaluated in the phylogenetic analysis. PCR amplification with ITS 4/ITS 5 primers generated bands ranging from 595 to 665 bp. Alignment of the ITS sequences was done using Bioedit. Neighbour-Joining (NJ) tree were constructed using Seaview programme (Figure
The authors express their thanks to the Scientific Investigation Projects Coordination Office of the Selçuk University (project no: 08201037) for financial support.
Representative specimens examined (*which used for DNA samples): – Seseli corymbosum: Turkey, C3 Antalya: Akseki, Pınarbası village, S of Gidefi Mountain, A.Duran 2970 (