Research Article |
Corresponding author: Shuichiro Tagane ( stagane29@gmail.com ) Academic editor: Thomas L.P. Couvreur
© 2015 Shuichiro Tagane, Son Van Dang, Tetsukazu Yahara, Hironori Toyama, Hop Tran.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Tagane S, Dang VS, Yahara T, Toyama H, Tran H (2015) Goniothalamus flagellistylus Tagane & V. S. Dang (Annonaceae), a new species from Mt. Hon Ba, Vietnam. PhytoKeys 50: 1-8. https://doi.org/10.3897/phytokeys.50.4427
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A new species, Goniothalamus flagellistylus Tagane & V. S. Dang, sp. nov. from Hon Ba Nature Reserve in Khanh Hoa Province, South Vietnam is described and illustrated. This species is most similar to Goniothalamus tortilipetalus M.R.Hend., but distinct in having 308–336 stamens (vs. ca. 170–260) and ca.120 carpels (vs. ca. 50–100) per flower, and Stigma and pseudostyles ca.8.5 mm (vs. 4–4.5 mm) long.
Annonaceae , Goniothalamus , Hon Ba Nature Reserve, Vietnam
The genus Goniothalamus (Blume) Hook. f. & Thomson, with more than 130 species (
During the botanical survey of Hon Ba Nature Reserve in South Vietnam, we encountered an undescribed species of Goniothalamus in Mt. Hon Ba at 400 m elevation. We here describe and illustrate this new species, Goniothalamus flagellistylus Tagane & V. S. Dang, sp. nov.
In order to verify the validity of this new species we undertook a thorough literature review, consulted specimens from the following herbaria FU, BKF, KYO, MBK and VNM, as well as online digitized plant specimens (e.g. JSTOR Global Plants).
The thickness of leaves, sepals and petals was measured using a digital caliper (Absolute Digimatic 547-401, Mitutoyo, Japan, resolution 0.001 mm).
For DNA isolation, leaf material was collected and desiccated using silica gel in the field. DNA was extracted using a modified CTAB method in which silica-dried leaves were ground in a TissueLyser (QIAGEN), and the powder washed five times with 1 mL buffer (0.1 M HEPES, pH 8.0; 2% mercaptoetanol; 1% PVP; 0.05 M ascorbic acid) before DNA extraction. We sequenced the partial genes for the large subunit ribulose-1,5-bisphosphate carboxylase oxygenase (rbcL) and maturase K (matK), following published protocols (
Similar to Goniothalamus tortilipetalus M.R.Hend., but differing from that species in having 308–336 stamens (vs. ca. 170–260) and ca.120 carpels (vs. ca. 50–100) per flower, and stigmas and pseudostyles ca.8.5 mm (vs. 4–4.5 mm) long.
Vietnam, Khanh Hoa Province, Hon Ba Nature Reserve, in evergreen forest near stream, 12°06.51'N, 108°59.23'E, alt. 400 m, Tagane S., Kanemitsu H., Dang V.S., Tran H. with Hanh N., Loi X.N., Thach N.D., Dinh N., Hieu P.N.H. V1497, 12 July 2014, Fl., holotype: KYO!; isotypes: BKF!, FU!, K!, VNM!, the herbarium of Hon Ba Nature Reserve!).
Small trees, 11 m tall, DBH 8 cm. Young twigs sparsely covered with brown hairs, soon glabrous, blackish when dry. Petioles 1–1.2(–1.5) cm long, 2.5–3.5 mm in diam., glabrous, black when dry. Leaf blades narrowly oblong-elliptic, 31–45 × 8.2–11.5 cm, length/width ratio 2.7–4.0, 185–225 µm thick, base acute to obtuse, margin entire, revolute when dry, apex acuminate, acumen ca. 1.5 cm long, leathery, slightly shiny above, glabrous on both surfaces; midribs impressed above, prominent below, glabrous on both surfaces, secondary veins 16–20 pairs, arising at an angle of 60–70 degrees from a midrib, prominent on both surface when dry, glabrous on both surfaces, tertiary veins distinct above, slightly distinct below. Flowers solitary, arising from main trunks and older branches, pendent; pedicels 19–25 mm long, 1.5–2.5 mm in diam., glabrous; bracts 3–4, very broadly triangular to hemiorbicular, ca. 1.6 mm long, brownish pubescent outside, glabrous inside, margin sometimes ciliate. Sepals ovate-triangular, 2.2–2.8 × 2.2–2.8 cm in flower, accrescent, increasing to 3.3 × 3.8 cm in fruit, 200–210 µm thick, basally connate, greenish in vivo, glabrous outside, sparsely covered with brown hairs inside, veins reticulated, distinct outside, indistinct inside. Outer petals oblong-ovate to narrowly ovate, 6–9. 2 × 2.2–3.1 cm, length/width ratio 2.7–3.4, 380–450 µm thick, greenish, glabrescent outside, sparsely covered with short brown hairs inside, except at base velutinous, veins faintly visible outside, indistinct inside. Inner petals rhombic, 1.6 × 0.7 cm, length/width ratio ca. 1.9, 1100–2100 µm thick, greenish, pubescent outside, velutinous inside with 12–14 basal grooves. Stamens 308–336 per flower, flattened-oblong, 3.8–4.8 × 0.6 mm, glabrous; connectives long-apiculate, 1.2–1.5 mm long, apiculate length 0.5–0.9 mm long, densely covered with cream-white hairs. Carpels ca. 120 per flower; ovary 1.4–1.7 × ca. 0.3 mm, densely covered with golden-brown hairs; stigmas and pseudostyles flagellate, ca.8.5 mm long, L-shaped curved in the middle, yellowish in vivo, blackish when dry, glabrous, tip awl-shaped. Fruits with persistent calyx, fruiting pedicels 2.7 cm long, 3–4.5 mm in diam. Monocarps 22, ellipsoid, 1.6–1.7 cm long, ca. 1.0 cm in diam., base attenuate, apex apiculate, glabrous, reddish-brown, pericarp ca. 0.5 mm thick when dry; stipes 0.6–1.2 mm long, ca. 2 mm in diam., glabrous. Seeds one per monocarp, 1.5 cm long, 0.8–1.0 cm in diam., yellowish brown, glabrous, seeds with copious surrounding mucilage.
Goniothalamus flagellistylus sp. nov. (a) Leafy branch, (b) portion of abaxial leaf surface, (c) flowers on main trunk, (d) vertical section of flowers, (e) apertures between inner petals, (f–g) mature fruit on older blanch, (h) holotype, (i) pedicel and sepals on old branch, (j) adaxial side of inner petal, (k) stamen, (l) carpel. (h–l) From Tagane et al. 1497. Scale bars (i, j) = 1 cm, (k, l) = 1 mm.
Mature flowers and fruits were collected in July and November, respectively.
This species is known only from Mt. Hon Ba, Khanh Hoa Province, South Vietnam. The small population was found on a slope in broad-leaved evergreen forest, ca. 100 m apart from a rapid river, where Ixonanthes reticulata Jack, Palaquium sp., Gironniera subaequalis Planch., Archidendron chevalieri (Kosterm.) I.C.Nielsen, Barringtonia augusta Kurz, Barringtonia macrostachya (Jack) Kurz, Camellia krempfii (Gagn.) Sealy, Streblus indicus (Bureau) Corner, Xerospermum noronhianum Blume and Pandanus fibrosus Gagnep. are dominated.
Vietnam, Khanh Hoa Province, Hon Ba Nature Reserve, in evergreen forest near stream, 12°06.51'N, 108°59.23'E, alt. 400 m, Toyama H., Tagane S., Dang V.S., Nagamasu H., Naiki A., Tran H., Yang C.J. with Cuong N.Q., Hieu H.N.P. V1972, 22 November 2014, Fr. (FU!, KYO!, NTU!, VNM!, the herbarium of Hon Ba Nature Reserve!).
The specific epithet is in reference to its flexuous styles which is too long to insert straight in the pollination chamber that formed by the inner petals.
Goniothalamus flagellistylus is morphologically similar to G. calvicarpus Craib, G. griffithii Hook.f. & Thomson, and G. tortilipetalus, all of which form a monophyletic group (
Morphological comparison between Goniothalamus flagellistylus sp. nov. and G. tortilipetalus (modified from Henderson 1933;
Characters | G. flagellistylus | G. tortilipetalus |
---|---|---|
Flowers position | On main trunk and older branches | On main trunk only |
Pedicel length | 19–25 mm | 20–37 mm |
Sepals in flowering | 22–28 by 22–28 mm | 19–31 by 15–26 mm |
Outer petals | 60–92 by 22–31 mm | 35–100 by 10–25 mm |
Inner petal length/width ratio | 1.9 | 2.2–3.1 |
Stamen number per flower | 308–336 | ~170–260 |
Carpel number per flower | 120 | ~50–100 |
Stigmas and pseudostyles | ca. 8.5 mm long | 4–4.5 mm long |
Ovary indument | densely hairy | (Very) densely hairy |
Data deficient. Goniothalamus flagellistylus is known from a single population, including only six individuals: only one produces flowers/fruits while the others are just saplings. This situation satisfies the CR (critically endangered) status in criterion D of IUCN Red List Categories (
The authors cordially thank staff of the Hon Ba Nature Reserve for their supports of field works in the protected area. Our gratitude goes to the curators and staff of the herbaria BKF, KYO, MBK, VNM for making their materials accessible. We also thank Xing Guo (University of Hong Kong) for comments on identification, Hidetoshi Nagamasu (KYO) for discussing the specific epithet of the species and Keiko Mase (Kyushu University) for DNA experiments. The present study was supported by the Environment Research and Technology Development Fund (S9) of the Ministry of the Environment, Japan.