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Research Article
Impatiens bokorensis (Balsaminaceae), a new species from Cambodia
expand article infoSeong-Hyun Cho, Bo-Yun Kim§, Han-Sol Park§, Chhang Phourin|, Young-Dong Kim§
‡ Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea, South
§ Hallym University, Chuncheon, Korea, South
| Forest Administration, Phnom Penh, Cambodia
Open Access

Abstract

Impatiens bokorensis, a new species of family Balsaminaceae from Phnum Bokor National Park in southwestern Cambodia, is described and illustrated. The species is similar to I. patula, but is readily distinguished by the orbicular-obovate dorsal petal, shorter pedicels and larger seeds.

Keywords

Phnum Bokor National Park, Endemic species, Impatiens, Cambodia

Introduction

Balsaminaceae is a family consisting of about 1,000 species mainly distributed in tropical Africa, Madagascar, southern India and Sri Lanka, the eastern Himalayas and southeastern Asia and is absent from Australia and South America (Song et al. 2003, Yuan et al. 2004, APG III 2009). This family includes annual or perennial herbs (more or less succulent) to sub-shrubs. It is distinguished from other families by strongly zygomorphic flowers with a spur on the adaxial sepal and a fleshy explosive-dehiscent capsule (Chen et al. 2007, APG III 2009). It comprises two genera: Hydrocera Blume ex Wight & Arn. (monotypic) and Impatiens L. with the most species.

Impatiens is classified into two subgenera (subgenus Impatiens Warb. and subgenus Acaulimpatiens Warb.) based on the presence or absence of cauline leaves. These two subgenera comprise 14 sections (subgenus Acaulimpatiens, two sections and subgenus Impatiens, 12 sections) mainly segregated by phyllotaxy, inflorescence and spur characters (Warburg and Reiche 1895, Utami 2009). Nonetheless, in a recent molecular phylogenetic study based on a nuclear ribosomal internal transcribed spacer (ITS) and plastid atpB-rbcL and trnL-F (Yu et al. 2015), Impatiens was classified into two subgenera (subgenus Clavicarpa S.X. Yu ex S.X. Yu & Wei Wang and Impatiens) with the subgenus Impatiens composed of seven sections (sect. Semeiocardium, sect. Racemosae, sect. Fasciculatae, sect. Tuberosae, sect. Scorpioidae, sect. Uniflorae and sect. Impatiens).

In Indochina, there are around 120 species of Impatiens and the present count includes approximately 40 species from Vietnam (Tardieu 1944, Ho 1999, Vietnam Plant Data Center 2016), around 60 species from Thailand (Grey-Wilson 1971, Shimizu 2000, Shimizu and Suksathan 2004, Chayamarit et al. 2006, Suksathan and Triboun 2009, Ruchisansakun et al. 2014) and 18 from Laos (Tardieu 1944, Newman et al. 2007, Newman 2008, Souvannakhoummane and Suksathan 2015). In Cambodia, at the beginning of the 20th century, six species were described, namely Impatiens cardiophylla Hook.f., I. diffusa Hook.f., I. notoptera Hook.f., I. relaxata Hook.f., I. vagans Hook.f. and I. zygosepala Hook.f. (Hooker 1908, 1909a, 1909b, 1911), whereas eight species are reported in the present account (Cho et al. 2016).

Except for I. balsamina and I. cardiophylla, most species in Cambodia have been considered endemic species, with I. relaxata, I. vagans and I. zygosepala restricted to a local area with only a very small number of specimens. There is a need to re-evaluate and resurvey areas of the previous collection of specimens through a detailed taxonomic study of each species.

During the recent floristic survey, one species of Impatiens was collected at Bokor National Park in Southwestern Cambodia that does not appear to be similar to previously reported species (Figures 1 & 2). It is most similar to Impatiens patula Craib from Thailand (Craib 1926, Shimizu 1970), but a comparison with the type specimens and descriptions revealed that it differs from I. patula and is therefore described here as a new species.

Figure 1.

Impatiens bokorensis A Flowering individual B Fruiting individual C–D Developing flower bud E Mature flower (before pollination) F–G Mature flower (after pollination) H Developing gynoecium I Petals J Androecium K Developing fruit L–M Seed N–O strigose-ciliate at leaf base: Cho et al. CB-3112, 3432. Illustration by Hye-Woo Shin.

Figure 2.

A–E Impatiens bokorensis A Habit B–C Flower D strigose-ciliate at leaf base E Capsule: Photos by Seong-Hyun Cho.

Taxonomy

Impatiens bokorensis S.H.Cho & B.Y.Kim, sp. nov.

Figures 1, 2

Type

CAMBODIA. Kampot Province, Phnum Bokor National Park, sandstone tables in evergreen forest margin, 10°38'20.8"N, 104°00'16.0"E, a.s.l. 1,050 m, 24 August 2015, with flowers, Cho S.H, Kim B.Y., Park H.S., Chhang Phourin CB-3112 (holotype HHU!, isotypes KB!, KRIB!, RUPP!).

Diagnosis

Impatiens bokorensis is most similar to the Thailand endemic species I. patula Craib in habit but is readily distinguished from the latter by the orbicular-obovate dorsal petal, shorter pedicels and larger seeds (Table 1).

Comparison of key features of Impatiens bokorensis and I. patula.

Taxonomic traits Impatiens bokorensis I. patula
Leaf
upper surface pubescent pubescent with scurfy hairs
lower surface glabrous to sparsely pubescent pubescent on nerves only or tomentose all over
strigose-ciliate at base 1–2.7 mm 1–1.4 mm
Pedicel 1.4–2.0 cm 2.3–3(–5) cm
Dorsal petal orbicular-obovate, ca. 6 × 8 mm, horned at apex, horn 2.2–2.3 mm long cordate, 7 mm long, horned at apex, horn 3 mm long
Seeds 3.8–4.6 × 2.6–3.2 mm 3.5 × 2.5 mm

Description

Herbs, annual, terrestrial, hermaphroditic. Stems erect, 15–40 cm tall, tinged purplish red, branched, glabrous or sparsely puberulous on the upper part. Leaves simple, alternate; petioles subsessile to 1.4 mm; leaf blade lanceolate to ovate-lanceolate, apex acuminate, base narrowly cuneate to attenuate, 2.5–7.0 × 0.6–2.0 cm, upper surface pubescent, lower surface glabrous to sparsely pubescent, secondary veins pinnate, 6 to 8 on each side of mid-vein, margin serrate, teeth mucronate and purple tinged, strigose-ciliate at base; strigose-ciliate, 4–14, 1–2.7 mm long, purple to purplish black, minutely puberulous at base. Flowers axillary, solitary, rarely 2 fascicled, zygomorphic, minutely puberulous; pedicels slender, erect, purplish red, 1.4–2.0 cm long, glabrous, bracteate at base; bracts linear, up to 4 mm; lateral sepals 2, linear-lanceolate, 2.5–3 mm long, glabrous; lower sepal funnel-form, pink, ca. 5 mm long, ca. 3 mm deep; spur 17–23 mm long, slightly curved; dorsal petal, orbicular-obovate, ca. 6 × 8 mm, horned at apex, horn 2.2–2.3 mm long; lateral united petals separate, bilobed, ca. 11 mm long; upper petals oblong, 6.0–6.5 × ca. 3.0 mm, minutely apiculate; lower petals, 9.0–9.3 × 3.5–3.8 mm; androecium ca. 2.8 × 1.6 mm; stamens 5, connate, surrounding gynoecium; filaments ca. 0.7 mm; ovary fusiform, pubescent, ca. 2.5 × 1.0 mm; style glabrous, ca. 0.2 mm long; stigma 5, ca. 0.25 mm long. Fruit a capsule, fusiform, ca. 15 × 6 mm, pubescent with scurfy hairs, 3[4]-seeded. Seeds obovoid, slightly compressed, 3.8–4.6 × 2.6–3.2 mm, pubescent with spirally sculptured hairs.

Specimen examined

CAMBODIA. 16 November 2015, with fruits, Cho et al. CB-3432 (HHU!, KRIB!); 2 September 2016, with flowers, Kim et al. CB-3537 (HHU!)

Phenology

Flowering specimens were collected in August and fruiting specimens in November.

Distribution and habitat

Impatiens bokorensis grows on sandstone tables in evergreen forest margins at 1,050 m a.s.l.. Endemic to southwestern Cambodia, I. bokorensis is at present known only in the type locality.

GenBank Accession No

Cho et al. CB-3432: KX171761 (ITS).

Conservation status

Impatiens bokorensis was collected in Phnum Bokor National Park in southwestern Cambodia. Until now, only one population, consisting of ca. 200 individuals, has been discovered in the park area; therefore, it is preliminarily classified as data deficient (DD) according to the IUCN Red List criteria (IUCN 2001).

Acknowledgements

We thank Ms. Hye-Woo Shin (http://www.hyewoo.com/) for preparing the line drawing. This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR201604201). Also, We would like to thanks Dr. Rajeev Singh and Dr. Mike Skinner for their valuable comments and suggestions.

References

  • Chayamarit K, Pooma R, Suddee S (2006) Thailand red data: plants. Office of Natural Resources and Environmental Policy and Planning, 1–256.
  • Chen Y, Akiyama S, Ohba H (2007) Impatiens L. In: Wu Z-Y, Raven PH (Eds) Flora of China, vol. 12. Science Press, Beijing & Missouri Botanical Garden Press, St. Louis, 43–113.
  • Cho S-H, Chhang P, Kim Y-D (2016) A checklist for the seed plants of Cambodia. National Institute of Biological Resources, Ministry of Environment, Incheon, 272 pp.
  • Craib WG (1926) Contributions to the Flora of Siam. Additamentum XVIII. Bulletin of Miscellaneous Information, vol. 1926(4). Royal Botanic Gardens, Kew, 154–174. https://doi.org/10.2307/4118686
  • Hooker JD (1908) Impatiens zygosepala Hook.f.. Hooker’s Icones Plantarum, vol. 29(3): t. 2857.
  • Hooker JD (1909a) Impatiens d’Indo-Chine. In: Lecomte H (Ed.) Notulae systematicae, Laboratoire de Phanérogamie, Muséum National d’Histoire Naturelle, Paris, 1: 10–14.
  • Hooker JD (1909b) On some species of Impatiens from Indo-China and the Malayan peninsula. Bulletin of Miscellaneous Information, Royal Gardens, Kew, 1: 1–12.
  • Hooker JD (1911) Balsaminacees. In: Lecomte H, Gagnepain F (Eds) Flore Generale de l’Indo-Chine (vol. 1), 611–629.
  • Ho PH (1999) Balsaminaceae. An Illustrated Flora of Vietnam, vol. 2. Ho Chi Minh City, 1–951. [In Vietnamese]
  • Shimizu T, Suksathan P (2004) Three new species of the Thai Impatiens (Balsaminaceae). Part 3. Bulletin of the National Science Museum, Series B, Botany 30(4): 165–171.
  • Shimizu T (1970) Contributions to the flora of Southeast Asia II: Impatiens of Thailand and Malaya. The southeast asian studies 8(2): 187–217.
  • Shimizu T (2000) New species of the Thai Impatiens (Balsaminacea) 2. Bulletin of the National Science Museum, Series B, Botany 26(2): 35–42.
  • Souvannakhoummane K, Suksathan P (2015) Two new species of Impatiens (Balsaminaceae) from north of Lao PDR. Taiwania 60(4): 175–180.
  • Suksathan P, Triboun P (2009) Ten new species of Impatiens (Balsaminaceae) from Thailand. Gardens’ Bulletin (Singapore) 61(1): 159–184
  • Tardieu BM (1944) Les Impatiens d’indochine, repartition, affinites et description d’especes nouvelles. Notulae Systematicae 11: 169–185.
  • Utami N (2009) The status of Semeiocardium Zoll. (Balsaminaceae). Reinwardtia 13(1): 21–23.
  • Warburg O, Reiche K (1896) Balsaminaceae. In: Engler HGA, Prantl KAE (Eds) Die natürlichen Pflanzenfamilien vol. 3 Wilhelm Engelmann, Leipzig, 1–468.
  • Yuan YM, Song Y, Geuten K, Rahelivololona E, Wohlhauser S, Fischer E, Smets E, Kupfer P (2004) Phylogeny and biogeography of Balsaminaceae inferred from ITS sequence data. Taxon 53(2): 391–403. https://doi.org/10.2307/4135617